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. 1967 Sep;104(3):725–730. doi: 10.1042/bj1040725

Effect of adenosine 3′,5′-(cyclic)-monophosphate on the synthesis of progestational steroids by rabbit ovarian tissue in vitro

J H Dorrington 1,*, R Kilpatrick 1
PMCID: PMC1271211  PMID: 6049918

Abstract

1. Adenosine 3′,5′-(cyclic)-monophosphate (3′,5′-AMP) stimulates the synthesis of progestational steroids by rabbit ovarian tissue in vitro. 2. Other adenosine phosphates fail to increase steroidogenesis. 3. The ratio of 20α-hydroxypregn-4-en-3-one to progesterone, the maximal response of the tissue, and the responses of separated corpora lutea and interstitial tissue produced by luteinizing hormone are closely paralleled by 3′,5′-AMP. 4. In tissues maximally stimulated by luteinizing hormone, 3′,5′-AMP fails to produce an additional response. 5. The addition of theophylline, an inhibitor of phosphodiesterase, potentiates the effects of 3′,5′-AMP and also luteinizing hormone. 6. The results obtained suggest that 3′,5′-AMP is a mediator of the action of luteinizing hormone on progestational steroid synthesis by rabbit ovarian tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dorrington J. H., Kilpatrick R. Effects of pituitary hormones on progestational hormone production by the rabbit ovary in vivo and in vitro. J Endocrinol. 1966 May;35(1):53–63. doi: 10.1677/joe.0.0350053. [DOI] [PubMed] [Google Scholar]
  2. HAYNES R. C., Jr, BERTHET L. Studies on the mechanism of action of the adrenocorticotropic hormone. J Biol Chem. 1957 Mar;225(1):115–124. [PubMed] [Google Scholar]
  3. HAYNES R. C., Jr, KORITZ S. B., PERON F. G. Influence of adenosine 3',5'-monophosphate on corticoid production by rat adrenal glands. J Biol Chem. 1959 Jun;234(6):1421–1423. [PubMed] [Google Scholar]
  4. HAYNES R. C., Jr, SUTHERLAND E. W., RALL T. W. The role of cyclic adenylic acid in hormone action. Recent Prog Horm Res. 1960;16:121–138. [PubMed] [Google Scholar]
  5. HAYNES R. C., Jr The activation of adrenal phosphorylase by the adrenocorticotropic hormone. J Biol Chem. 1958 Nov;233(5):1220–1222. [PubMed] [Google Scholar]
  6. HILLIARD J., ARCHIBALD D., SAWYER C. H. Gonadotropic activation of prevulatory synthesis and release of progestin in the rabbit. Endocrinology. 1963 Jan;72:59–66. doi: 10.1210/endo-72-1-59. [DOI] [PubMed] [Google Scholar]
  7. KORITZ S. B. Some observations on the stimulation in vitro of corticoid production by adenosine 3',5'-monophosphate in rat adrenals. Biochim Biophys Acta. 1962 Jun 18;60:179–181. doi: 10.1016/0006-3002(62)90385-2. [DOI] [PubMed] [Google Scholar]
  8. ORLOFF J., HANDLER J. S. The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder. J Clin Invest. 1962 Apr;41:702–709. doi: 10.1172/JCI104528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RALL T. W., SUTHERLAND E. W. Formation of a cyclic adenine ribonucleotide by tissue particles. J Biol Chem. 1958 Jun;232(2):1065–1076. [PubMed] [Google Scholar]
  10. RALL T. W., SUTHERLAND E. W. The regulatory role of adenosine-3', 5'-phosphate. Cold Spring Harb Symp Quant Biol. 1961;26:347–354. doi: 10.1101/sqb.1961.026.01.042. [DOI] [PubMed] [Google Scholar]
  11. SAVARD K., MARSH J. M., RICE B. F. GONADOTROPINS AND OVARIAN STEROIDOGENESIS. Recent Prog Horm Res. 1965;21:285–365. [PubMed] [Google Scholar]
  12. SUTHERLAND E. W., RALL T. W. Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles. J Biol Chem. 1958 Jun;232(2):1077–1091. [PubMed] [Google Scholar]

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