Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Sep;104(3):735–741. doi: 10.1042/bj1040735

Pepsinogen D

A fourth proteolytic zymogen from pig gastric mucosa

D Lee 1,*, A P Ryle 1
PMCID: PMC1271213  PMID: 4167464

Abstract

Methods are described for the isolation and purification of pepsinogen D, a minor zymogen occurring to the extent of about 5% of the potential proteolytic activity in neutral extracts of the pig gastric mucosa. The physical and chemical properties of this zymogen indicate that it is very similar to, if not identical with, dephosphopepsinogen.

Full text

PDF
735

Images in this article

738Fig. 3.
on p.738

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen R. J. The estimation of phosphorus. Biochem J. 1940 Jun;34(6):858–865. doi: 10.1042/bj0340858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BAGLIONI C. An improved method for the fingerprinting of human hemoglobin. Biochim Biophys Acta. 1961 Apr 1;48:392–396. doi: 10.1016/0006-3002(61)90490-5. [DOI] [PubMed] [Google Scholar]
  3. BLACKBURN S., LOWTHER A. G. The separation of N-2:4-dinitrophenly amino-acids on paper chromatograms. Biochem J. 1951 Jan;48(1):126–128. doi: 10.1042/bj0480126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hanley W. B., Boyer S. H., Naughton M. A. Electrophoretic and functional heterogeneity of pepsinogen in several species. Nature. 1966 Mar 5;209(5027):996–1002. doi: 10.1038/209996a0. [DOI] [PubMed] [Google Scholar]
  6. KUSHNER I., RAPP W., BURTIN P. ELECTROPHORETIC AND IMMUNOCHEMICAL DEMONSTRATION OF THE EXISTENCE OF FOUR HUMAN PEPSINOGENS. J Clin Invest. 1964 Oct;43:1983–1993. doi: 10.1172/JCI105072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LEVCHUK T. P., OREKHOVICH V. N. POLUCHENIE I NEKOTORYE SVO ISTVA KURINOGO PEPSINA. Biokhimiia. 1963 Nov-Dec;28:1004–1010. [PubMed] [Google Scholar]
  8. LEVY A. L. A paper chromatographic method for the quantitative estimation of amino-acids. Nature. 1954 Jul 17;174(4420):126–127. doi: 10.1038/174126a0. [DOI] [PubMed] [Google Scholar]
  9. Lee D., Ryle A. P. Pepsin D. A minor component of commercial pepsin preparations. Biochem J. 1967 Sep;104(3):742–748. doi: 10.1042/bj1040742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. PERLMANN G. E. Enzymic dephosphorylation of pepsin and pepsinogen. J Gen Physiol. 1958 Jan 20;41(3):441–450. doi: 10.1085/jgp.41.3.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RYLE A. P. PEPSINOGEN B: THE ZYMOGEN OF PEPSIN B. Biochem J. 1965 Jul;96:6–16. doi: 10.1042/bj0960006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ryle A. P., Hamilton M. P. Pepsinogen C and pepsin C. Further purification and amino acid composition. Biochem J. 1966 Oct;101(1):176–183. doi: 10.1042/bj1010176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ryle A. P. Minor proteases in the stomach of the pig. Biochem J. 1966 Feb;98(2):485–487. doi: 10.1042/bj0980485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SANGER F., HOCQUARD E. Formation of dephospho-ovalbumin as an intermediate in the biosynthesis of ovalbumin. Biochim Biophys Acta. 1962 Aug 27;62:606–607. doi: 10.1016/0006-3002(62)90253-6. [DOI] [PubMed] [Google Scholar]
  15. SEIJFFERS M. J., SEGAL H. L., MILLER L. L. SEPARATION OF PEPSIN I, PEPSIN II A, PEPSIN II B, AND PEPSIN III FROM HUMAN GASTRIC MUCOSA. Am J Physiol. 1963 Dec;205:1099–1105. doi: 10.1152/ajplegacy.1963.205.6.1099. [DOI] [PubMed] [Google Scholar]
  16. SEIJFFERS M. J., SEGAL H. L., MILLER L. L. SEPARATION OF PEPSINOGEN I, PEPSINOGEN II, AND PEPSINOGEN III FROM HUMAN GASTRIC MUCOSA. Am J Physiol. 1963 Dec;205:1106–1112. doi: 10.1152/ajplegacy.1963.205.6.1106. [DOI] [PubMed] [Google Scholar]
  17. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. VAN VUNAKIS H., HERRIOTT R. M. Structural changes associated with the conversion of pepsinogen to pepsin. II. The N-terminal amino acid residues of pepsin and pepsinogen; the amino acid composition of pepsinogen. Biochim Biophys Acta. 1957 Mar;23(3):600–608. doi: 10.1016/0006-3002(57)90382-7. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES