Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1967 Sep;104(3):1082–1088. doi: 10.1042/bj1041082

The isolation of purified neurosecretory granules from bovine pituitary posterior lobes

Comparison of granule protein constituents with those of neurophysin

C R Dean 1, D B Hope 1
PMCID: PMC1271255  PMID: 6049859

Abstract

1. A procedure for the isolation of highly purified neurosecretory granules from the posterior lobe of the bovine pituitary gland is described. The preparation was free from contamination by the mitochondrial enzyme succinate dehydrogenase and the lysosomal enzyme cathepsin. 2. The biological activities of the neurosecretory granules were measured: the oxytocic activity was 11·61±1·30units and the pressor activity was 10·73±1·74units/mg. of protein. 3. A lysate of the isolated granules was shown to contain two proteins that appear to be identical with two of the constituents of neurophysin. 4. The constituents of neurophysin not present in neurosecretory granules could not be detected in any other subcellular fraction. It is suggested that the components of neurophysin not present in the neurosecretory granules arise as a result of the degradation of the two granular proteins.

Full text

PDF
1082

Images in this article

1086Fig. 3.
on p.1086
1086-1Plate 1 (a)
on p.1086-1
1086-1Plate 1 (b)
on p.1086-1
1086-1Plate 1 (c)
on p.1086-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ACHER R., CHAUVET J., OLIVRY G. Sur l'existence éventuelle d'une hormone unique neurohypophysaire. I. Relations entre l'ocytocine, la vasopressine et la protéine de Van Dyke extraites de la neurohypophyse du boeuf. Biochim Biophys Acta. 1956 Dec;22(3):421–427. doi: 10.1016/0006-3002(56)90050-6. [DOI] [PubMed] [Google Scholar]
  2. Andrews P. The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem J. 1965 Sep;96(3):595–606. doi: 10.1042/bj0960595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BANGHAM D. R., MUSSETT M. V. Third international standard for posterior pituitary; re-named third international standard for oxytocic, vasopressor and antidiuretic substances in 1956. Bull World Health Organ. 1958;19(2):325–340. [PMC free article] [PubMed] [Google Scholar]
  4. BARER R., HELLER H., LEDERIS K. THE ISOLATION, IDENTIFICATION AND PROPERTIES OF THE HORMONAL GRANULES OF THE NEUROHYPOPHYSIS. Proc R Soc Lond B Biol Sci. 1963 Oct 22;158:388–416. doi: 10.1098/rspb.1963.0054. [DOI] [PubMed] [Google Scholar]
  5. Breslow E., Abrash L. The binding of oxytocin and oxytocin analogues by purified bovine neurophysins. Proc Natl Acad Sci U S A. 1966 Aug;56(2):640–646. doi: 10.1073/pnas.56.2.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DEKANSKI J. The quantitative assay of vasopressin. Br J Pharmacol Chemother. 1952 Dec;7(4):567–572. doi: 10.1111/j.1476-5381.1952.tb00723.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FERGUSON K. A., WALLACE A. L. Starch-gel electrophoresis of anterior pituitary hormones. Nature. 1961 May 13;190:629–630. doi: 10.1038/190629a0. [DOI] [PubMed] [Google Scholar]
  8. Frankland B. T., Hollenberg M. D., Hope D. B., Schacter B. A. Dissociation of oxytocin and vasopressin from their carrier protein by chromatography on sephadex G-25. Br J Pharmacol Chemother. 1966 Feb;26(2):502–510. doi: 10.1111/j.1476-5381.1966.tb01931.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ginsburg M., Ireland M. The role of neurophysin in the transport and release of neurohypophysical hormones. J Endocrinol. 1966 Jul;35(3):289–298. doi: 10.1677/joe.0.0350289. [DOI] [PubMed] [Google Scholar]
  10. Hollenberg M. D., Hope D. B. Fractionation of neurophysin by molecular-sieve and ion-exchange chromatography. Biochem J. 1967 Jul;104(1):122–127. doi: 10.1042/bj1040122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LABELLA F. S., REIFFENSTEIN R. J., BEAULIEU G. Subcellular fractionation of bovine posterior pituitary glands by centrifugation. Arch Biochem Biophys. 1963 Mar;100:399–408. doi: 10.1016/0003-9861(63)90104-8. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MUNSICK R. A. Effect of magnesium ion on the response of the rat uterus to neurohypophysial hormones and analogues. Endocrinology. 1960 Mar;6:451–457. doi: 10.1210/endo-66-3-451. [DOI] [PubMed] [Google Scholar]
  14. PARDOE A. U., WEATHERALL M. The intracellular localization of oxytocic and vasopressor substances in the pituitary glands of rats. J Physiol. 1955 Jan 28;127(1):201–212. doi: 10.1113/jphysiol.1955.sp005249. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PENNINGTON R. J. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase. Biochem J. 1961 Sep;80:649–654. doi: 10.1042/bj0800649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PORTEOUS J. W., CLARK B. THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE. Biochem J. 1965 Jul;96:159–171. doi: 10.1042/bj0960159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SAWYER W. H. Neurophypophysial hormones. Pharmacol Rev. 1961 Jun;13:225–277. [PubMed] [Google Scholar]
  18. SCHAPIRO S., STJARNE L. Evidence for the granular localization of posterior pituitary hormones. Nature. 1961 Feb 25;189:669–669. doi: 10.1038/189669a0. [DOI] [PubMed] [Google Scholar]
  19. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WEINSTEIN H., MALAMED S., SACHS H. Isolation of vasopressin-containing granules from the neurohypophysis of the dog. Biochim Biophys Acta. 1961 Jun 24;50:386–389. doi: 10.1016/0006-3002(61)90349-3. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES