Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1981 Feb;311:401–409. doi: 10.1113/jphysiol.1981.sp013592

The effect of intracerebroventricular injections of morphine on vasopressin release in the rat.

L A Aziz, M L Forsling, C J Woolf
PMCID: PMC1275417  PMID: 7264975

Abstract

1. An investigation was carried out to determine the effect of intracerebroventricular (I.C.V.) micro-injections of morphine on vasopressin (AVP) release in the urethane-anaesthetized rat. 2. Plasma AVP levels at different time intervals, following I.C.V. injection of 10-150 microgram morphine, were measured by radioimmunoassay. The effect of I.C.V. micro-injections of morphine on urine outflow was also studied in a group of water-loaded rats. 3. The vasopressin response to I.C.V. micro-injections of morphine was both dose- and time-dependent. High dose of 50 and 150 microgram morphine produced short latency stimulation of AVP release, followed by a fall. The low dose of 10 microgram morphine produced only a long latency inhibition. The most consistent response of I.C.V. injection of morphine was an inhibition of release. 4. Both stimulatory and inhibitory effects of morphine on vasopressin release were naloxone reversible and stereospecific. 5. I.C.V. micro-injections of morphine produced a dose-dependent rise in mean arterial blood pressure of short latency. Naloxone (0.5 mg/kg) completely abolished the rise seen with 10 microgram morphine and diminished the rise with 50 microgram. 6. Doses of 10 and 50 microgram morphine injected I.C.V. produced an immediate antidiuresis in water-loaded rats under urethane anaesthesia. 7. The vasopressin response to I.C.V. micro-injections of morphine is independent of the effects on the cardiovascular system and may involve different opiate receptor populations. The results also suggest the possibility that opiate receptors with different affinities for morphine may be responsible for the stimulatory and inhibitory effects of morphine on vasopressin release.

Full text

PDF
401

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bisset G. W., Black A. J., Hilton P. J., Jones N. F., Kanjanapothi D., Montgomery M. C. Proceedings: An antibody to vasopressin in man. J Physiol. 1974 May;239(1):57P–58P. [PubMed] [Google Scholar]
  2. Bisset G. W., Chowdrey H. S., Feldberg W. Release of vasopressin by enkephalin. Br J Pharmacol. 1978 Mar;62(3):370–371. doi: 10.1111/j.1476-5381.1978.tb08472.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang K. J., Cooper B. R., Hazum E., Cuatrecasas P. Multiple opiate receptors: different regional distribution in the brain and differential binding of opiates and opioid peptides. Mol Pharmacol. 1979 Jul;16(1):91–104. [PubMed] [Google Scholar]
  4. Clarke G., Wood P., Merrick L., Lincoln D. W. Opiate inhibition of peptide release from the neurohumoral terminals of hypothalamic neurones. Nature. 1979 Dec 13;282(5740):746–748. doi: 10.1038/282746a0. [DOI] [PubMed] [Google Scholar]
  5. Fee A. R. Studies on water diuresis: Part I. The effect of decerebration, anaesthesia and morphia upon water diuresis. J Physiol. 1929 Aug 28;68(1):39–44. doi: 10.1113/jphysiol.1929.sp002593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Feldberg W., Wei E. Central cardiovascular effects of enkephalins and C-fragment of lipotropin [proceedings]. J Physiol. 1978 Jul;280:18P–18P. [PubMed] [Google Scholar]
  7. Feldberg W., Wei E. Central sites at which morphine acts when producing cardiovascular effects [proceedings]. J Physiol. 1978 Feb;275:57P–57P. [PubMed] [Google Scholar]
  8. Firemark H. M., Weitzman R. E. Effects of beta-endorphin, morphine and naloxone on arginine vasopressin secretion and the electroencephalogram. Neuroscience. 1979;4(12):1895–1902. doi: 10.1016/0306-4522(79)90063-0. [DOI] [PubMed] [Google Scholar]
  9. Haldar J., Sawyer W. H. Inhibition of oxytocin release by morphine and its analogs. Proc Soc Exp Biol Med. 1978 Mar;157(3):476–480. doi: 10.3181/00379727-157-40080. [DOI] [PubMed] [Google Scholar]
  10. Huidobro F., Huidobro-Toro J. Antidiuretic effect of morphine and vasopressin in morphine tolerant and non-tolerant rats, differential effects on urine composition. Eur J Pharmacol. 1979 Oct 26;59(1-2):55–64. doi: 10.1016/0014-2999(79)90024-4. [DOI] [PubMed] [Google Scholar]
  11. Iversen L. L., Iversen S. D., Bloom F. E. Opiate receptors influence vasopressin release from nerve terminals in rat neurohypophysis. Nature. 1980 Mar 27;284(5754):350–351. doi: 10.1038/284350a0. [DOI] [PubMed] [Google Scholar]
  12. Karonen S. L., Mörsky P., Siren M., Seuderling U. An enzymatic solid-phase method for trace iodination of proteins and peptides with 125-iodine. Anal Biochem. 1975 Jul;67(1):1–10. doi: 10.1016/0003-2697(75)90266-3. [DOI] [PubMed] [Google Scholar]
  13. Lightman S. L., Forsling M. Evidence for dopamine as an inhibitor of vasoprotein release in man. Clin Endocrinol (Oxf) 1980 Jan;12(1):39–46. doi: 10.1111/j.1365-2265.1980.tb03130.x. [DOI] [PubMed] [Google Scholar]
  14. Rossier J., Battenberg E., Pittman Q., Bayon A., Koda L., Miller R., Guillemin R., Bloom F. Hypothalamic enkephalin neurones may regulate the neurohypophysis. Nature. 1979 Feb 22;277(5698):653–655. doi: 10.1038/277653a0. [DOI] [PubMed] [Google Scholar]
  15. Simantov R., Snyder S. H. Opiate receptor binding in the pituitary gland. Brain Res. 1977 Mar 18;124(1):178–184. doi: 10.1016/0006-8993(77)90877-0. [DOI] [PubMed] [Google Scholar]
  16. Weitzman R. E., Fisher D. A., Minick S., Ling N., Guillemin R. beta-Endorphin secretion of arginine vasopressin in vivo. Endocrinology. 1977 Nov;101(5):1643–1646. doi: 10.1210/endo-101-5-1643. [DOI] [PubMed] [Google Scholar]
  17. van Wimersma Greidanus T. B., Thody T. J., Verspaget H., de Rotte G. A., Goedemans H. J., Croiset G., van Ree J. M. Effects of morphine and beta-endorphin on basal and elevated plasma levels of alpha-MSH and vasopressin. Life Sci. 1979 Feb 12;24(7):579–585. doi: 10.1016/0024-3205(79)90153-x. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES