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. 1994 Mar;66(3 Pt 1):554–560. doi: 10.1016/s0006-3495(94)80872-0

Teaching active transport at the turn of the twenty-first century: recent discoveries and conceptual changes.

G Inesi 1
PMCID: PMC1275754  PMID: 8011889

Abstract

The conceptual advances introduced by recent discoveries in the field of active transport have triggered a transition from a "black box" approach to a "mechanistic" approach. At present, treating this subject in the graduate setting requires consideration of equilibrium and kinetic experimentation, protein chemistry, mutational analysis and molecular structure, with the aim of defining the "transport machine."

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bigelow D. J., Inesi G. Contributions of chemical derivatization and spectroscopic studies to the characterization of the Ca2+ transport ATPase of sarcoplasmic reticulum. Biochim Biophys Acta. 1992 Dec 11;1113(3-4):323–338. doi: 10.1016/0304-4157(92)90005-u. [DOI] [PubMed] [Google Scholar]
  2. Clarke D. M., Loo T. W., Inesi G., MacLennan D. H. Location of high affinity Ca2+-binding sites within the predicted transmembrane domain of the sarcoplasmic reticulum Ca2+-ATPase. Nature. 1989 Jun 8;339(6224):476–478. doi: 10.1038/339476a0. [DOI] [PubMed] [Google Scholar]
  3. Clarke D. M., Maruyama K., Loo T. W., Leberer E., Inesi G., MacLennan D. H. Functional consequences of glutamate, aspartate, glutamine, and asparagine mutations in the stalk sector of the Ca2+-ATPase of sarcoplasmic reticulum. J Biol Chem. 1989 Jul 5;264(19):11246–11251. [PubMed] [Google Scholar]
  4. Green N. M., Taylor W. R., Brandl C., Korczak B., MacLennan D. H. Structural and mechanistic implications of the amino acid sequence of calcium-transporting ATPases. Ciba Found Symp. 1986;122:93–114. doi: 10.1002/9780470513347.ch7. [DOI] [PubMed] [Google Scholar]
  5. Holmes M. A., Matthews B. W. Structure of thermolysin refined at 1.6 A resolution. J Mol Biol. 1982 Oct 5;160(4):623–639. doi: 10.1016/0022-2836(82)90319-9. [DOI] [PubMed] [Google Scholar]
  6. Inesi G., Kurzmack M., Coan C., Lewis D. E. Cooperative calcium binding and ATPase activation in sarcoplasmic reticulum vesicles. J Biol Chem. 1980 Apr 10;255(7):3025–3031. [PubMed] [Google Scholar]
  7. Inesi G., Kurzmack M., Lewis D. Kinetic and equilibrium characterization of an energy-transducing enzyme and its partial reactions. Methods Enzymol. 1988;157:154–190. doi: 10.1016/0076-6879(88)57074-x. [DOI] [PubMed] [Google Scholar]
  8. Inesi G., Kurzmack M., Verjovski-Almeida S. ATPase phosphorylation and calcium ion translocation in the transient state of sarcoplasmic reticulum activity. Ann N Y Acad Sci. 1978 Apr 28;307:224–227. doi: 10.1111/j.1749-6632.1978.tb41947.x. [DOI] [PubMed] [Google Scholar]
  9. Inesi G. Mechanism of calcium transport. Annu Rev Physiol. 1985;47:573–601. doi: 10.1146/annurev.ph.47.030185.003041. [DOI] [PubMed] [Google Scholar]
  10. Levy D., Seigneuret M., Bluzat A., Rigaud J. L. Evidence for proton countertransport by the sarcoplasmic reticulum Ca2(+)-ATPase during calcium transport in reconstituted proteoliposomes with low ionic permeability. J Biol Chem. 1990 Nov 15;265(32):19524–19534. [PubMed] [Google Scholar]
  11. MacLennan D. H., Brandl C. J., Korczak B., Green N. M. Amino-acid sequence of a Ca2+ + Mg2+-dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequence. Nature. 1985 Aug 22;316(6030):696–700. doi: 10.1038/316696a0. [DOI] [PubMed] [Google Scholar]
  12. Pickart C. M., Jencks W. P. Energetics of the calcium-transporting ATPase. J Biol Chem. 1984 Feb 10;259(3):1629–1643. [PubMed] [Google Scholar]
  13. Stokes D. L., Green N. M. Structure of CaATPase: electron microscopy of frozen-hydrated crystals at 6 A resolution in projection. J Mol Biol. 1990 Jun 5;213(3):529–538. doi: 10.1016/s0022-2836(05)80213-x. [DOI] [PubMed] [Google Scholar]
  14. Toyoshima C., Sasabe H., Stokes D. L. Three-dimensional cryo-electron microscopy of the calcium ion pump in the sarcoplasmic reticulum membrane. Nature. 1993 Apr 1;362(6419):467–471. doi: 10.1038/362469a0. [DOI] [PubMed] [Google Scholar]
  15. Zhang Z., Sumbilla C., Lewis D., Inesi G. High sensitivity to site directed mutagenesis of the peptide segment connecting phosphorylation and Ca2+ binding domains in the Ca2+ transport ATPase. FEBS Lett. 1993 Dec 6;335(2):261–264. doi: 10.1016/0014-5793(93)80742-d. [DOI] [PubMed] [Google Scholar]

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