Skip to main content
NCBI home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1975 Jul;39(3):332–339.

The kinetics of hematopoiesis in the light horse III. The hematological response to hemolytic anemia.

H J Lumsden, V E Valli, B J McSherry, G A Robinson, M J Claxton
PMCID: PMC1277467  PMID: 1139414

Abstract

The hematological response to acetylphenylhydrazine hemolytic anemia was studied in three standardbred horses. The lifespan of erythrocytes produced during the most severe phase of the anemia were measured with 75-selenomethionine and found to be 144 days as compared to the 139 day lifespan in response to hemorrhagic anemia or 155 days in normal standardbred horses measured previously using the same technique. The erythrocyte counts returned to initial values in 42 days (37, 34 and 54 days) a mean erythrocyte production of 6.4 times 10-12 erythrocytes/day. The mean hemoglobin production was 0.31 gm/kg body weight/day as compared to 0.11 gm Hb/kg/day previously observed in response to hemorrhagic anemia. The mean increase in erythrocyte mean cell volume was 12 mu-3 during the acute response phase to hemolytic anemia in contrast to the absence of a significant increase in the mean cell volume as previously observed during response to hemorrhagic anemia. Free Heinz bodies separated from erythrocytes during the acute phase could not be differentiated from platelets on the hemocytometer counting chamber with standard techniques.

Full text

PDF
332

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERLIN N. I., LOTZ C. Life span of the red blood cell of the rat following acute hemorrhage. Proc Soc Exp Biol Med. 1951 Dec;78(3):788–790. doi: 10.3181/00379727-78-19220. [DOI] [PubMed] [Google Scholar]
  2. BUSH J. A., JENSEN W. N., ASHENBRUCKER H., CARTWRIGHT G. E., WINTROBE M. M. The kinetics of iron metabolism in swine with various experimentally induced anemias. J Exp Med. 1956 Jan 1;103(1):161–171. doi: 10.1084/jem.103.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baird J. D., Hutchins D. R., Lepherd E. E. Phenothiazine poisoning in a thoroughbred horse. Aust Vet J. 1970 Oct;46(10):496–499. doi: 10.1111/j.1751-0813.1970.tb09172.x. [DOI] [PubMed] [Google Scholar]
  4. CLINE M. J., BERLIN N. I. Red blood cell life span using DFP as a cohort label. Blood. 1962 Jun;19:715–723. [PubMed] [Google Scholar]
  5. COLEMAN D. H., STEVENS A. R., Jr, DODGE H. T., FINCH C. A. Rate of blood regeneration after blood loss. AMA Arch Intern Med. 1953 Sep;92(3):341–349. doi: 10.1001/archinte.1953.00240210045006. [DOI] [PubMed] [Google Scholar]
  6. CROSBY W. H., AKEROYD J. H. The limit of hemoglobin synthesis in hereditary hemolytic anemia; its relation to the excretion of bile pigment. Am J Med. 1952 Sep;13(3):273–283. doi: 10.1016/0002-9343(52)90282-9. [DOI] [PubMed] [Google Scholar]
  7. Card R. T., Valberg L. S. Characteristics of shortened survival of stress erythrocytes in the rabbit. Am J Physiol. 1967 Sep;213(3):566–572. doi: 10.1152/ajplegacy.1967.213.3.566. [DOI] [PubMed] [Google Scholar]
  8. Carter E. I., Valli V. E., McSherry B. J., Milne F. J., Robinson G. A., Lumsden J. H. The kinetics of hematopoiesis in the light horse. I. The lifespan of peripheral blood cells in the normal horse. Can J Comp Med. 1974 Jul;38(3):303–313. [PMC free article] [PubMed] [Google Scholar]
  9. FINCH C. A., HANSON M. L., DONOHUE D. M. Kinetics of erythropoiesis. A comparison of response to anemia induced by phenylhydrazine and by blood loss. Am J Physiol. 1959 Oct;197:761–764. doi: 10.1152/ajplegacy.1959.197.4.761. [DOI] [PubMed] [Google Scholar]
  10. Freedman M. L., Karpatkin S. Requirement of iron for platelet protein synthesis. Biochem Biophys Res Commun. 1973 Sep 18;54(2):475–481. doi: 10.1016/0006-291x(73)91445-9. [DOI] [PubMed] [Google Scholar]
  11. Ganzoni A. M., Oakes R., Hillman R. S. Red cell aging in vivo. J Clin Invest. 1971 Jul;50(7):1373–1378. doi: 10.1172/JCI106619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HARLEY J. D., MAUER A. M. Studies on the formation of Heinz bodies. II. The nature and significance of Heinz bodies. Blood. 1961 Apr;17:418–433. [PubMed] [Google Scholar]
  13. Hamstra R. D., Block M. H. Erythropoiesis in response to blood loss in man. J Appl Physiol. 1969 Oct;27(4):503–507. doi: 10.1152/jappl.1969.27.4.503. [DOI] [PubMed] [Google Scholar]
  14. Lumsden J. H., Valli V. E., McSherry B. J., Robinson G. A., Claxton M. J. The kinetics of hematopoiesis in the light horse II. The hematological response to hemorrhagic anemia. Can J Comp Med. 1975 Jul;39(3):324–331. [PMC free article] [PubMed] [Google Scholar]
  15. MARCILESE N. A., VALSECCHI R. M., FIGUEIRAS H. D., CAMBEROS H. R., VARELA J. E. NORMAL BLOOD VOLUMES IN THE HORSE. Am J Physiol. 1964 Jul;207:223–227. doi: 10.1152/ajplegacy.1964.207.1.223. [DOI] [PubMed] [Google Scholar]
  16. MILLETTE R. L., GLOWACKI E. R. IN VIVO MATURATION OF IMMATURE RETICULOCYTES TRANSFUSED INTO A NORMAL RABBIT. Nature. 1964 Dec 19;204:1207–1209. doi: 10.1038/2041207b0. [DOI] [PubMed] [Google Scholar]
  17. McSherry B. J., Roe C. K., Milne F. J. The hematology of phenothiazine poisoning in horses. Can Vet J. 1966 Jan;7(1):3–12. [PMC free article] [PubMed] [Google Scholar]
  18. NEUBERGER A., NIVEN J. S. F. Haemoglobin formation in rabbits. J Physiol. 1951 Feb;112(3-4):292–310. doi: 10.1113/jphysiol.1951.sp004530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nagai K., Kakishita E. Destruction of immature erythrocytes measured by bilirubin excretion. Blood. 1969 May;33(5):717–726. [PubMed] [Google Scholar]
  20. Nagel R. L., Ranney H. M. Drug-induced oxidative denaturation of hemoglobin. Semin Hematol. 1973 Oct;10(4):269–278. [PubMed] [Google Scholar]
  21. OWEN J. A., BETTER F. C., HOBAN J. A simple method for the determination of serum haptoglobins. J Clin Pathol. 1960 Mar;13:163–164. doi: 10.1136/jcp.13.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Osbaldiston G. W., Coffman J. R., Kruckenberg S. M. Biochemical differentiation of equine anemias. J Am Vet Med Assoc. 1970 Aug 1;157(3):322–325. [PubMed] [Google Scholar]
  23. Pierce K. R., Joyce J. R., England R. B., Jones L. P. Acute hemolytic anemia caused by wild onion poisoning in horses. J Am Vet Med Assoc. 1972 Feb 1;160(3):323–327. [PubMed] [Google Scholar]
  24. REIFF R. H., NUTTER J. Y., DONOHUE D. M., FINCH C. A. The relative number of marrow reticulocytes. Am J Clin Pathol. 1958 Sep;30(3):199–203. doi: 10.1093/ajcp/30.3.199. [DOI] [PubMed] [Google Scholar]
  25. STOHLMAN F., Jr Erythropoiesis. N Engl J Med. 1962 Aug 16;267:342–348. doi: 10.1056/NEJM196208162670707. [DOI] [PubMed] [Google Scholar]
  26. Shattil S. J., Cooper R. A. Maturation of macroreticulocyte membranes in vivo. J Lab Clin Med. 1972 Feb;79(2):215–227. [PubMed] [Google Scholar]
  27. Simpson C. F. Fate of erythrocytes containing Heinz bodies in the spleen and liver of dogs and turkeys. Vet Pathol. 1971;8(2):118–129. doi: 10.1177/030098587100800203. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES