Abstract
The stability of hog cholera virus through five freeze-thaw cycles in the presence and absence of dimethyl sulfoxide was studied. In the absence of dimethyl sulfoxide the hog cholera virus titer was reduced 52% to 91% following successive freezing and thawing cycles. However, when dimethyl sulfoxide was added to the viral suspension the virus titer appeared to remain the same after the same number of freezing and thawing cycles.
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Selected References
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- Carbrey E. A., Stewart W. C., Kresse J. I. Confirmation of hog cholera diagnosis by rapid serum-neutralization technique. J Am Vet Med Assoc. 1969 Dec 15;155(12):2201–2210. [PubMed] [Google Scholar]
- LOVELOCK J. E. The denaturation of lipid-protein complexes as a cause of damage by freezing. Proc R Soc Lond B Biol Sci. 1957 Dec 17;147(929):427–433. doi: 10.1098/rspb.1957.0062. [DOI] [PubMed] [Google Scholar]
- Phillips C. E. In vitro potency tests for anti-hog cholera antibodies: a test for anti-hog cholera serums and a test for herd exposure. Am J Vet Res. 1968 May;29(5):1097–1102. [PubMed] [Google Scholar]
- Wallis C., Melnick J. L. Stabilization of enveloped viruses by dimethyl sulfoxide. J Virol. 1968 Sep;2(9):953–954. doi: 10.1128/jvi.2.9.953-954.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]