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. 1976 Apr;40(2):166–170.

Effects of glucagon and insulin on lipolysis and ketogenesis in sheep.

R P Brockman
PMCID: PMC1277543  PMID: 1000386

Abstract

The hepatic and portal productions of acetoacetate and beta-hydroxybutyrate and lipolysis were studied in normal and insulin-controlled alloxan-diabetic sheep. Since hyperinsulinemia is associated with glucagon administration, the latter group of sheep were used to maintain constant plasma insulin levels. After control values were obtained glucagon was infused intraportally at 90 mug/hr for two hours. The ketone body production by portal drained viscera was not significantly affected by glucagon. In alloxanized sheep, glucagon significantly (P less than 0.01) increased net hepatic production of acetoacetate (from -0.54 +/- 0.08 to 0.46 +/- 0.07 g/hr). Lipolysis also increased. However, in the normal sheep, hyperinsulinemia prevented any stimulatory effect of glucagon on hepatic ketogenesis and lipolysis. Therefore, while glucagon appears capable of stimulating ketogenesis andlipolysis, these effects are readily suppressed by insulin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balasse E. O., Havel R. J. Evidence for an effect of inulin on the peripheral utilization of ketone bodies in dogs. J Clin Invest. 1971 Apr;50(4):801–813. doi: 10.1172/JCI106551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassett J. M. The effects of glucagon on plasma concentrations of insulin, growth hormone, glucose, and free fatty acids in sheep: comparison with the effects of catecholamines. Aust J Biol Sci. 1971 Apr;24(2):311–320. doi: 10.1071/bi9710311. [DOI] [PubMed] [Google Scholar]
  3. Bergman E. N. Glycerol turnover in the nonpregnant and ketotic pregnant sheep. Am J Physiol. 1968 Oct;215(4):865–873. doi: 10.1152/ajplegacy.1968.215.4.865. [DOI] [PubMed] [Google Scholar]
  4. Bergman E. N. Hyperketonemia-ketogenesis and ketone body metabolism. J Dairy Sci. 1971 Jun;54(6):936–948. doi: 10.3168/jds.S0022-0302(71)85950-7. [DOI] [PubMed] [Google Scholar]
  5. Bergman E. N., Roe W. E., Kon K. Quantitative aspects of propionate metabolism and gluconeogenesis in sheep. Am J Physiol. 1966 Sep;211(3):793–799. doi: 10.1152/ajplegacy.1966.211.3.793. [DOI] [PubMed] [Google Scholar]
  6. Brockman R. P., Bergman E. N., Joo P. K., Manns J. G. Effects of glucagon and insulin on net hepatic metabolism of glucose precursors in sheep. Am J Physiol. 1975 Nov;229(5):1344–1349. doi: 10.1152/ajplegacy.1975.229.5.1344. [DOI] [PubMed] [Google Scholar]
  7. Brockman R. P., Bergman E. N. Quantitative aspects of insulin secretion and its hepatic and renal removal in sheep. Am J Physiol. 1975 Nov;229(5):1338–1343. doi: 10.1152/ajplegacy.1975.229.5.1338. [DOI] [PubMed] [Google Scholar]
  8. Katz M. L., Bergman E. N. A method for simultaneous cannulation of the major splanchnic blood vessels of the sheep. Am J Vet Res. 1969 Apr;30(4):655–661. [PubMed] [Google Scholar]
  9. Katz M. L., Bergman E. N. Simultaneous measurements of hepatic and portal venous blood flow in the sheep and dog. Am J Physiol. 1969 Apr;216(4):946–952. doi: 10.1152/ajplegacy.1969.216.4.946. [DOI] [PubMed] [Google Scholar]
  10. Kaufman C. F., Bergman E. N. Renal ketone body metabolism and gluconeogenesis in normal and hypoglycemic sheep. Am J Physiol. 1974 Apr;226(4):827–832. doi: 10.1152/ajplegacy.1974.226.4.827. [DOI] [PubMed] [Google Scholar]
  11. Liljenquist J. E., Bomboy J. D., Lewis S. B., Sinclair-Smith B. C., Felts P. W., Lacy W. W., Crofford O. B., Liddle G. W. Effects of glucagon on lipolysis and ketogenesis in normal and diabetic men. J Clin Invest. 1974 Jan;53(1):190–197. doi: 10.1172/JCI107537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Luthman J., Jonson G. Short-term hormonal effects on blood glucose and non-esterified fatty acids in female sheep. Acta Vet Scand. 1972;13(1):112–127. [PubMed] [Google Scholar]
  13. Mayes P. A., Felts J. M. Regulation of fat metabolism of the liver. Nature. 1967 Aug 12;215(5102):716–718. doi: 10.1038/215716a0. [DOI] [PubMed] [Google Scholar]
  14. Persson B. Determination of plasma acetoacetate and D-beta-hydroxybutyrate in new-born infants by an enzymatic fluorometric micro-method. Scand J Clin Lab Invest. 1970 Jan;25(1):9–18. doi: 10.3109/00365517009046184. [DOI] [PubMed] [Google Scholar]
  15. Weinstein I., Klausner H. A., Heimberg M. The effect of concentration of glucagon on output of triglyceride, ketone bodies, glucose, and urea by the liver. Biochim Biophys Acta. 1973 Feb 14;296(2):300–309. doi: 10.1016/0005-2760(73)90088-x. [DOI] [PubMed] [Google Scholar]

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