Skip to main content
NCBI home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1978 Jul;42(3):278–285.

Demonstration of parvovirus in Canadian swine and antigenic relationships with isolates from other countries.

G M Ruckerbauer, G C Dulac, P Boulanger
PMCID: PMC1277639  PMID: 356941

Abstract

A Canadian isolate of porcine parvovirus, isolated from cultured pig thyroid cells, was shown to be antigenically indistinguishable from a British (59e/63) and a German (G10/1) strain when treated by the modified direct complement-fixation, the hemagglutination-inhibition and the fluorescent antibody tests. These tests also revealed that antibodies to parvoviruses were detectable in a large proportion of the conventionally raised pigs in the provinces of Quebec and Ontario. Cell cultures, prepared from tissues collected in a slaughterhouse, were often found to be infected with parvovirus. In cell cultures the infection was demonstrated more effectively by immunofluorescence than by the hemagglutination test.

Full text

PDF
278

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boulanger P., Bannister G. L., Greig A. S., Gray D. P., Ruckerbauer G. M., Willis N. G. African swine fever. IV. Demonstration of the viral antigen by means of immunofluorescence. Can J Comp Med Vet Sci. 1967 Jan;31(1):16–23. [PMC free article] [PubMed] [Google Scholar]
  2. Boulanger P., L'Ecuyer C. Enzootic pneumonia of pigs: complement-fixation tests for the detection of mycoplasma antibodies in the serum of immunized rabbits and infected swine. Can J Comp Med. 1968 Oct;32(4):547–554. [PMC free article] [PubMed] [Google Scholar]
  3. Cartwright S. F., Lucas M., Huck R. A. A small haemagglutinating porcine DNA virus. I. Isolation and properties. J Comp Pathol. 1969 Jul;79(3):371–377. doi: 10.1016/0021-9975(69)90053-x. [DOI] [PubMed] [Google Scholar]
  4. Cartwright S. F., Lucas M., Huck R. A. A small haemaggultinating porcine DNA virus. II. Biological and serological studies. J Comp Pathol. 1971 Jan;81(1):145–155. doi: 10.1016/0021-9975(71)90067-3. [DOI] [PubMed] [Google Scholar]
  5. Croghan D. L., Matchett A., Koski T. A. Isolation of porcine parvovirus from commercial trypsin. Appl Microbiol. 1973 Sep;26(3):431–433. doi: 10.1128/am.26.3.431-433.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dulac G. C., Ruckerbauer G. M., Boulanger P. Transmissible gastroenteritis: demonstration of the virus from field specimens by means of cell culture and pig inoculation. Can J Comp Med. 1977 Oct;41(4):357–363. [PMC free article] [PubMed] [Google Scholar]
  7. Greig A. S., Bannister G. L., Mitchell D., Corner A. H. Studies on Pathogenic Porcine Enteroviruses: II. Isolation of Virus in Tissue Culture from Brain and Feces of Clinical Cases. Can J Comp Med Vet Sci. 1961 Jun;25(6):142–150. [PMC free article] [PubMed] [Google Scholar]
  8. Hallauer C., Siegl G., Kronauer G. Parvoviruses as contaminants of permanent human cell lines. 3. Biological properties of the isolated viruses. Arch Gesamte Virusforsch. 1972;38(4):366–382. doi: 10.1007/BF01262827. [DOI] [PubMed] [Google Scholar]
  9. Hierholzer J. C., Suggs M. T. Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions. Appl Microbiol. 1969 Nov;18(5):816–823. doi: 10.1128/am.18.5.816-823.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huygelen C., Peetermans J. Isolation of a hemagglutinating picornavirus from a primary swine kidney cell culture. Arch Gesamte Virusforsch. 1967;20(2):260–262. doi: 10.1007/BF01241281. [DOI] [PubMed] [Google Scholar]
  11. Johnson R. H. A search for parvoviridae (picodnaviridae). Vet Rec. 1969 Jan 4;84(1):19–20. doi: 10.1136/vr.84.1.19. [DOI] [PubMed] [Google Scholar]
  12. Johnson R. H., Collings D. F. Experimental infection of piglets and pregnant gilts with a parvovirus. Vet Rec. 1969 Oct 18;85(16):446–447. doi: 10.1136/vr.85.16.446. [DOI] [PubMed] [Google Scholar]
  13. Johnson R. H. Isolation of swine papvovirus in Queensland. Aust Vet J. 1973 Mar;49(3):157–159. doi: 10.1111/j.1751-0813.1973.tb06768.x. [DOI] [PubMed] [Google Scholar]
  14. Mayr A., Bachmann P. A., Siegl G., Mahnel H., Sheffy B. E. Characterization of a small porcine DNA virus. Arch Gesamte Virusforsch. 1968;25(1):38–51. doi: 10.1007/BF01243088. [DOI] [PubMed] [Google Scholar]
  15. Mengeling W. L. Porcine parvovirus: properties and prevalence of a strain isolated in the United States. Am J Vet Res. 1972 Nov;33(11):2239–2248. [PubMed] [Google Scholar]
  16. Mitchell A. G. Production de Porcs Exempts d'Organismes Pathogenes (EOPS) Primaires et Etablissement d'un Troupeau de Porcs EOPS Secondaires. Can J Comp Med Vet Sci. 1962 Dec;26(12):279–284. [PMC free article] [PubMed] [Google Scholar]
  17. Witte K. H., Easterday B. C. Isolation and propagation of the virus of transmissible gastroenteritis of pigs in various pig cell cultures. Arch Gesamte Virusforsch. 1967;20(3):327–350. doi: 10.1007/BF01241952. [DOI] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES