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. 1978 Jul;42(3):316–321.

Effect of heat stable and heat labile Escherichia coli enterotoxins and cholera toxin in combination with theophylline on unidirectional sodium and chloride flux in the small intestine of weanling swine.

D L Hamilton, G W Forsyth, W E Roe, N O Nielsen
PMCID: PMC1277644  PMID: 356942

Abstract

The effect of heat stable and heat labile Escherichia coli enterotoxins or cholera toxin in combination with theophylline on net water, sodium and chloride and unidirectional sodium and chloride fluxes was examined in acute isolated loops of jejunum of weanling swine. The effect of heat stable enterotoxin in combination with theophylline was determined in loops located in the proximal jejunum, while combinations of theophylline and either heat labile enterotoxin or cholera toxin were studied in the distal jejunum. In each situation the addition of theophylline resulted in an additive rather than a synergistic increment of intestinal secretory activity. This study implies that the intestinal adenyl cyclase system and enterotoxin induced intestinal secretion may not be directly related in the swine small intestine.

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Selected References

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  1. BERGER E. Y., STEELE J. M. The calculation of transfer rates in two compartment systems not in dynamic equilibrium. J Gen Physiol. 1958 Jul 20;41(6):1135–1152. doi: 10.1085/jgp.41.6.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banwell J. G., Sherr H. Effect of bacterial enterotoxins on the gastrointestinal tract. Gastroenterology. 1973 Sep;65(3):467–497. [PubMed] [Google Scholar]
  3. Carpenter C. C. Cholera and other enterotoxin-related diarrheal diseases. J Infect Dis. 1972 Nov;126(5):551–564. doi: 10.1093/infdis/126.5.551. [DOI] [PubMed] [Google Scholar]
  4. Carpenter C. C., Jr Cholera enterotoxin--recent investigations yield insights into transport processes. Am J Med. 1971 Jan;50(1):1–7. doi: 10.1016/0002-9343(71)90198-7. [DOI] [PubMed] [Google Scholar]
  5. Donta S. T., King M., Sloper K. Induction of steroidogenesis in tissue culture by cholera enterotoxin. Nat New Biol. 1973 Jun 20;243(129):246–247. doi: 10.1038/newbio243246a0. [DOI] [PubMed] [Google Scholar]
  6. Donta S. T., Moon H. W., Whipp S. C. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture. Science. 1974 Jan 25;183(4122):334–336. doi: 10.1126/science.183.4122.334. [DOI] [PubMed] [Google Scholar]
  7. Epstein S. E., Levey G. S., Skelton C. L. Adenyl cyclase and cyclic AMP. Biochemical links in the regulation of myocardial contractility. Circulation. 1971 Mar;43(3):437–450. doi: 10.1161/01.cir.43.3.437. [DOI] [PubMed] [Google Scholar]
  8. Evans D. J., Jr, Chen L. C., Curlin G. T., Evans D. G. Stimulation of adenyl cyclase by Escherichia coli enterotoxin. Nat New Biol. 1972 Apr 5;236(66):137–138. doi: 10.1038/newbio236137a0. [DOI] [PubMed] [Google Scholar]
  9. Field M: Intestinal secretion: effect of cyclic AMP and its role in cholera. N Engl J Med. 1971 May 20;284(20):1137–1144. doi: 10.1056/NEJM197105202842008. [DOI] [PubMed] [Google Scholar]
  10. Field M., Fromm D., al-Awqati Q., Greenough W. B., 3rd Effect of cholera enterotoxin on ion transport across isolated ileal mucosa. J Clin Invest. 1972 Apr;51(4):796–804. doi: 10.1172/JCI106874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grand R. J., Torti F. M., Jaksina S. Development of intestinal adenyl cyclase and its response to cholera enterotoxin. J Clin Invest. 1973 Aug;52(8):2053–2059. doi: 10.1172/JCI107389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Guerrant R. L., Chen L. C., Sharp G. W. Intestinal adenyl-cyclase activity in canine cholera: correlation with fluid accumulation. J Infect Dis. 1972 Apr;125(4):377–381. doi: 10.1093/infdis/125.4.377. [DOI] [PubMed] [Google Scholar]
  13. Guerrant R. L., Ganguly U., Casper A. G., Moore E. J., Pierce N. F., Carpenter C. C. Effect of Escherichia coli on fluid transport across canine small bowel. Mechanism and time-course with enterotoxin and whole bacterial cells. J Clin Invest. 1973 Jul;52(7):1707–1714. doi: 10.1172/JCI107352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hamilton D. L., Roe W. E., Nielsen N. O. Effect of heat stable and heat labile Escherichia coli enterotoxins, cholera toxin and theophylline on unidirectional sodium and chloride fluxes in the proximal and distal jejunum of weanling swine. Can J Comp Med. 1977 Jul;41(3):306–317. [PMC free article] [PubMed] [Google Scholar]
  15. Hirschhorn N., Rosenberg I. H. Sodium-potassium stimulated adenosine triphosphatase of the small intestine of man: studies in cholera and other diarrheal diseases. J Lab Clin Med. 1968 Jul;72(1):28–39. [PubMed] [Google Scholar]
  16. Kantor H. S., Tao P., Gorbach S. L. Stimulation of intestinal adenyl cyclase by Escherichia coli enterotoxin: comparison of strains from an infant and an adult with diarrhea. J Infect Dis. 1974 Jan;129(1):1–9. doi: 10.1093/infdis/129.1.1. [DOI] [PubMed] [Google Scholar]
  17. Kimberg D. V., Field M., Johnson J., Henderson A., Gershon E. Stimulation of intestinal mucosal adenyl cyclase by cholera enterotoxin and prostaglandins. J Clin Invest. 1971 Jun;50(6):1218–1230. doi: 10.1172/JCI106599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Liddle G. W., Hardman J. G. Cyclic adenosine monophosphate as a mediator of hormone action. N Engl J Med. 1971 Sep 2;285(10):560–566. doi: 10.1056/NEJM197109022851007. [DOI] [PubMed] [Google Scholar]
  19. Parkinson D. K., Ebel H., DiBona D. R., Sharp G. W. Localization of the action of cholera toxin on adenyl cyclase in mucosal epithelial cells of rabbit intestine. J Clin Invest. 1972 Sep;51(9):2292–2298. doi: 10.1172/JCI107039. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pierce N. F., Carpenter C. C., Jr, Elliott H. L., Greenough W. B., 3rd Effects of prostaglandins, theophylline, and cholera exotoxin upon transmucosal water and electrolyte movement in the canine jejunum. Gastroenterology. 1971 Jan;60(1):22–32. [PubMed] [Google Scholar]
  21. Pierce N. F., Greenough W. B., 3rd, Carpenter C. C., Jr Vibrio cholerae enterotoxin and its mode of action. Bacteriol Rev. 1971 Mar;35(1):1–13. doi: 10.1128/br.35.1.1-13.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sharp G. W. Action of cholera toxin on fluid and electrolyte movement in the small intestine. Annu Rev Med. 1973;24:19–23. doi: 10.1146/annurev.me.24.020173.000315. [DOI] [PubMed] [Google Scholar]
  23. Sharp G. W., Hynie S., Ebel H., Parkinson D. K., Witkum P. Properties of adenylate cyclase in mucosal cells of the rabbit ileum and the effect of cholera toxin. Biochim Biophys Acta. 1973 Jun 6;309(2):339–348. doi: 10.1016/0005-2744(73)90032-6. [DOI] [PubMed] [Google Scholar]
  24. Sharp G. W., Hynie S. Stimulation of intestinal adenyl cyclase by cholera toxin. Nature. 1971 Jan 22;229(5282):266–269. doi: 10.1038/229266a0. [DOI] [PubMed] [Google Scholar]
  25. Sladen G. E. The pathogenesis of cholera and some wider implications. Gut. 1973 Aug;14(8):671–680. doi: 10.1136/gut.14.8.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Smith H. W., Gyles C. L. The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. J Med Microbiol. 1970 Aug;3(3):387–401. doi: 10.1099/00222615-3-3-387. [DOI] [PubMed] [Google Scholar]

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