Abstract
An evaluation was made of a rapid semiautomated method for determining fibrinogen level in swine plasma. This method, referred to as thrombin time method or fibrometer method, is based on the principle that when thrombin is added to suitably diluted plasma, the time of clotting is linearly related to the fibrinogen concentration. The linear regression model for the standard curve prepared using swine plasma had an r value of 0.998. A comparison between the fibrometer and the Grannis methods done on 189 swine plasma samples showed good correlation between these two mehtods (r value 0.847). It was concluded that although the fibrometer method may not be as precise as the Grannis method, it would still be acceptable for clinical use in swine.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burmester H. B., Aulton K., Horsfield G. I. Evaluation of a rapid method for the determination of plasma fibrinogen. J Clin Pathol. 1970 Feb;23(1):43–46. doi: 10.1136/jcp.23.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grannis G. F. Plasma fibrinogen: determination, normal values, physiopathologic shifts, and fluctuations. Clin Chem. 1970 Jun;16(6):486–494. [PubMed] [Google Scholar]
- Huhn R. G., Osweiler G. D., Switzer W. P. Application of the orbital sinus bleeding technique to swine. Lab Anim Care. 1969 Jun;19(3):403–405. [PubMed] [Google Scholar]
- SCHNEIDER C. L. Rapid estimation of plasma fibrinogen concentration and its use as a guide to therapy of intravascular defibrination. Am J Obstet Gynecol. 1952 Jul;64(1):141–147. doi: 10.1016/s0002-9378(16)38744-0. [DOI] [PubMed] [Google Scholar]
- Stevens D. J., Sanfelippo M. J. Evaluation of three methods for plasma fibrinogen determination. Am J Clin Pathol. 1973 Aug;60(2):182–187. doi: 10.1093/ajcp/60.2.182. [DOI] [PubMed] [Google Scholar]
- Thomson G. W., McSherry B. J., Valli V. E. Evaluation of a rapid method of determination of plasma fibrinogen. Can J Comp Med. 1974 Jul;38(3):266–270. [PMC free article] [PubMed] [Google Scholar]