Abstract
1. The influence upon mammalian renal epithelial transport of L-nicotine was studied in different types of experiments. 2. The kidney in situ (rat), when infused with nicotine (1 mg hr-1 kg-1 I.V.), lowered its absolute and fractional K+-absorption significantly and reversibly, but Na+-absorption did not change. Effects on glomerular function and an irreversible effect upon epithelial Na+ and K+ absorption were prevailing at higher infused amounts (10 or 50 mg hr-1 kg-1). 3. Single dissected nephron segments (collecting tubule, rabbit) were perfused in vitro, and the Na+ and K+-transtubular net flux was measured while L-nicotine (50 ng/ml.) had been added to the contraluminal side of the epithelium. Both, Na+-absorption and K+-secretion were decreased reversibly. 4. The activity of the Na+-K+-activated ATPase was significantly decreased in the cortical collecting tubule and in the proximal convoluted tubule of the rabbit after incubation of single in vitro dissected nephron segments with L-nicotine (50 or 100 ng/ml.). In contrast, nicotine added to a homogenate of renal cortical tissue had no effect on the Na+-transport enzyme or on key enzymes of glycolysis and gluconeogenesis. 5. These observations on the kidney in situ and on defined, perfused and non-perfused nephrons in vitro suggest that L-nicotine has dose-dependent, direct epithelial and mediated systemic effects on mammalian renal ion transport.
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Selected References
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- Boudry J. F., Stoner L. C., Burg M. B. Effect of acid lumen pH on potassium transport in renal cortical collecting tubules. Am J Physiol. 1976 Jan;230(1):239–244. doi: 10.1152/ajplegacy.1976.230.1.239. [DOI] [PubMed] [Google Scholar]
- Dinno M. A., Ando M., Dinno F. H., Huang K. C., Rehm W. S. Effect of nicotine on gastric acid secretion: evidence of electrogenic pump theory. Am J Physiol. 1977 Mar;232(3):E251–E257. doi: 10.1152/ajpendo.1977.232.3.E251. [DOI] [PubMed] [Google Scholar]
- Horster M., Larsson L. Mechanisms of fluid absorption during proximal tubule development. Kidney Int. 1976 Nov;10(5):348–363. doi: 10.1038/ki.1976.121. [DOI] [PubMed] [Google Scholar]
- Horster M. Loop of Henle functional differentiation: in vitro perfusion of the isolated thick ascending segment. Pflugers Arch. 1978 Dec 15;378(1):15–24. doi: 10.1007/BF00581953. [DOI] [PubMed] [Google Scholar]
- Malnic G., De Mello Aires M., Giebisch G. Potassium transport across renal distal tubules during acid-base disturbances. Am J Physiol. 1971 Oct;221(4):1192–1208. doi: 10.1152/ajplegacy.1971.221.4.1192. [DOI] [PubMed] [Google Scholar]
- Michael H. Principles of nephron differentiation. Am J Physiol. 1978 Nov;235(5):F387–F393. doi: 10.1152/ajprenal.1978.235.5.F387. [DOI] [PubMed] [Google Scholar]
- Schmidt U., Horster M. Na-K-activated ATPase: activity maturation in rabbit nephron segments dissected in vitro. Am J Physiol. 1977 Jul;233(1):F55–F60. doi: 10.1152/ajprenal.1977.233.1.F55. [DOI] [PubMed] [Google Scholar]
- Schmidt U., Schmid H., Funk B., Dubach U. C. The function of Na, K-ATPase in single portions of the rat nephron. Ann N Y Acad Sci. 1974;242(0):489–500. doi: 10.1111/j.1749-6632.1974.tb19112.x. [DOI] [PubMed] [Google Scholar]
- Schmidt U., Schmid H., Guder W. G. Liver cell heterogeneity. The distribution of fructose-bisphosphatase in fed and fasted rats and in man. Hoppe Seylers Z Physiol Chem. 1978 Feb;359(2):193–198. [PubMed] [Google Scholar]
- Stoner L. C., Burg M. B., Orloff J. Ion transport in cortical collecting tubule; effect of amiloride. Am J Physiol. 1974 Aug;227(2):453–459. doi: 10.1152/ajplegacy.1974.227.2.453. [DOI] [PubMed] [Google Scholar]