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. 1980 Feb;299:533–552. doi: 10.1113/jphysiol.1980.sp013141

Interaction between nerve-related acetylcholine and bath applied agonists at the frog end-plate.

A Feltz, A Trautmann
PMCID: PMC1279241  PMID: 6966689

Abstract

1. The interaction between acetylcholine (ACh) and carbachol (CCh) has been studied at the frog end-plate. The conditioning agonist, CCh, can cause desensitization (reduction of the ACh test response) and potentiation (increase of the test response). 2. Nerve-evoked end-plate currents (e.p.c.s), minature e.p.c.s and "slow" responses to ACh ionophoresis can all be potentiated by bath or ionophoretically applied CCh. 3. Since potentiation was found to be particularly visible at low temperatures, most experiments were performed at 5-8 degrees C. Potentiation results in an increase of both e.p.c. amplitude and e.p.c. decay time. Potentiated e.p.c.s teminate with a slow tail, the amplitude of which shows a high voltage sensitivity. Potentiation increases with CCh concentration (range studied 0-100 microM). It appears to persist throughout the application of CCh, even when desensitization is apparently the dominant phenomenon. 4. It is suggested that cross-potentiation of ACh by CCh results from the formation of intermediate non-conducting CCh-receptor complexes which have a high probability of being subsequently activated by ACh, yielding a conducting ACh-CCh-receptor complex. 5. Desensitization induced by fast bath application of CCh (or ACh) develops in two phases and can be fitted by the sum of two exponentials. Their time constants are in the second and the minute range, respectively. 6. The possibility that the slow phase is linked to the presence of agonist inside the cell is rejected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams P. R. A study of desensitization using voltage clamp. Pflugers Arch. 1975 Oct 28;360(2):135–144. doi: 10.1007/BF00580536. [DOI] [PubMed] [Google Scholar]
  2. Adams P. R., Sakmann B. Decamethonium both opens and blocks endplate channels. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2994–2998. doi: 10.1073/pnas.75.6.2994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson C. R., Stevens C. F. Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction. J Physiol. 1973 Dec;235(3):655–691. doi: 10.1113/jphysiol.1973.sp010410. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ascher P., Marty A., Neild T. O. Life time and elementary conductance of the channels mediating the excitatory effects of acetylcholine in Aplysia neurones. J Physiol. 1978 May;278:177–206. doi: 10.1113/jphysiol.1978.sp012299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Colquhoun D., Large W. A., Rang H. P. An analysis of the action of a false transmitter at the neuromuscular junction. J Physiol. 1977 Apr;266(2):361–395. doi: 10.1113/jphysiol.1977.sp011772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colquhoun D., Range H. P. Effects of inhibitors of the binding of iodinated alpha-bungarotoxin to acetylcholine receptors in rat muscle. Mol Pharmacol. 1976 Jul;12(4):519–535. [PubMed] [Google Scholar]
  7. Constanti A., Nistri A. A study of the interactions between glutamate and aspartate at the lobster neuromuscular junction. Br J Pharmacol. 1978 Apr;62(4):495–505. doi: 10.1111/j.1476-5381.1978.tb07753.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Crawford A. C., McBurney R. N. The termination of transmitter action at the crustacean excitatory neuromuscular junction. J Physiol. 1977 Jul;268(3):711–729. doi: 10.1113/jphysiol.1977.sp011878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Creese R., England J. M. Decamethonium in depolarized muscle and the effects of tubocurarine. J Physiol. 1970 Sep;210(2):345–361. doi: 10.1113/jphysiol.1970.sp009214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DEL CASTILLO J., KATZ B. Interaction at end-plate receptors between different choline derivatives. Proc R Soc Lond B Biol Sci. 1957 May 7;146(924):369–381. doi: 10.1098/rspb.1957.0018. [DOI] [PubMed] [Google Scholar]
  11. DEL CASTILLO J., KATZ B. On the localization of acetylcholine receptors. J Physiol. 1955 Apr 28;128(1):157–181. doi: 10.1113/jphysiol.1955.sp005297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dreyer F., Peper K. The acetylcholine sensitivity in the vicinity of the neuromuscular junction of the frog. Pflugers Arch. 1974 May 6;348(4):273–286. doi: 10.1007/BF00589217. [DOI] [PubMed] [Google Scholar]
  13. Dudel J. Aspartate and other inhibitors of excitatory synaptic transmission in crayfish muscle. Pflugers Arch. 1977 May 6;369(1):7–16. doi: 10.1007/BF00580803. [DOI] [PubMed] [Google Scholar]
  14. Eisenberg R. S., Howell J. N., Vaughan P. C. The maintenance of resting potentials in glycerol-treated muscle fibres. J Physiol. 1971 May;215(1):95–102. doi: 10.1113/jphysiol.1971.sp009459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Feltz A., Large W. A., Trautmann A. Analysis of atropine action at the frog neutromuscular junction. J Physiol. 1977 Jul;269(1):109–130. doi: 10.1113/jphysiol.1977.sp011895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Gage P. W., Van Helden D. Effects of permeant monovalent cations on end-plate channels. J Physiol. 1979 Mar;288:509–528. [PMC free article] [PubMed] [Google Scholar]
  17. Hartzell H. C., Kuffler S. W., Yoshikami D. Post-synaptic potentiation: interaction between quanta of acetylcholine at the skeletal neuromuscular synapse. J Physiol. 1975 Oct;251(2):427–463. doi: 10.1113/jphysiol.1975.sp011102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. KATZ B., MILEDI R. THE EFFECT OF CALCIUM ON ACETYLCHOLINE RELEASE FROM MOTOR NERVE TERMINALS. Proc R Soc Lond B Biol Sci. 1965 Feb 16;161:496–503. doi: 10.1098/rspb.1965.0017. [DOI] [PubMed] [Google Scholar]
  19. KATZ B., THESLEFF S. A study of the desensitization produced by acetylcholine at the motor end-plate. J Physiol. 1957 Aug 29;138(1):63–80. doi: 10.1113/jphysiol.1957.sp005838. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Katz B., Miledi R. The binding of acetylcholine to receptors and its removal from the synaptic cleft. J Physiol. 1973 Jun;231(3):549–574. doi: 10.1113/jphysiol.1973.sp010248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Katz B., Miledi R. The characteristics of 'end-plate noise' produced by different depolarizing drugs. J Physiol. 1973 May;230(3):707–717. doi: 10.1113/jphysiol.1973.sp010213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Katz B., Miledi R. The nature of the prolonged endplate depolarization in anti-esterase treated muscle. Proc R Soc Lond B Biol Sci. 1975 Dec 31;192(1106):27–38. doi: 10.1098/rspb.1975.0149. [DOI] [PubMed] [Google Scholar]
  23. Katz B., Miledi R. The statistical nature of the acetycholine potential and its molecular components. J Physiol. 1972 Aug;224(3):665–699. doi: 10.1113/jphysiol.1972.sp009918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Magazanik L. G., Vyskocit F. The effect of temperature on desensitization kinetics at the post-synaptic membrane of the frog muscle fibre. J Physiol. 1975 Jul;249(2):285–300. doi: 10.1113/jphysiol.1975.sp011016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Magleby K. L., Stevens C. F. The effect of voltage on the time course of end-plate currents. J Physiol. 1972 May;223(1):151–171. doi: 10.1113/jphysiol.1972.sp009839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Magleby K. L., Terrar D. A. Factors affecting the time course of decay of end-plate currents: a possible cooperative action of acetylcholine on receptors at the frog neuromuscular junction. J Physiol. 1975 Jan;244(2):467–495. doi: 10.1113/jphysiol.1975.sp010808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Meech R. W. The sensitivity of Helix aspersa neurones to injected calcium ions. J Physiol. 1974 Mar;237(2):259–277. doi: 10.1113/jphysiol.1974.sp010481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Miyamoto M. D. The actions of cholinergic drugs on motor nerve terminals. Pharmacol Rev. 1977 Sep;29(3):221–247. [PubMed] [Google Scholar]
  29. Rang H. P., Ritter J. M. On the mechanism of desensitization at cholinergic receptors. Mol Pharmacol. 1970 Jul;6(4):357–382. [PubMed] [Google Scholar]
  30. STEPHENSON R. P. A modification of receptor theory. Br J Pharmacol Chemother. 1956 Dec;11(4):379–393. doi: 10.1111/j.1476-5381.1956.tb00006.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Scubon-Mulieri B., Parsons R. L. Desensitization onset and recovery at the potassium-depolarized frog neuromuscular junction are voltage sensitive. J Gen Physiol. 1978 Mar;71(3):285–299. doi: 10.1085/jgp.71.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Shank R. P., Freeman A. R. Cooperative interaction of glutamate and aspartate with receptors in the neuromuscular excitatory membrane in walking limbs of the lobster. J Neurobiol. 1975 May;6(3):289–303. doi: 10.1002/neu.480060305. [DOI] [PubMed] [Google Scholar]
  33. Vigny M., Bon S., Massoulié J., Leterrier F. Active-site catalytic efficiency of acetylcholinesterase molecular forms in Electrophorus, torpedo, rat and chicken. Eur J Biochem. 1978 Apr 17;85(2):317–323. doi: 10.1111/j.1432-1033.1978.tb12241.x. [DOI] [PubMed] [Google Scholar]
  34. Vyskocil F., Magazanik L. G. The desensitization of postjunctional muscle membrane after intracellular application of membrane stabilizers and snake venom polypeptides. Brain Res. 1972 Dec 24;48:417–419. doi: 10.1016/0006-8993(72)90202-8. [DOI] [PubMed] [Google Scholar]
  35. Weber M., David-Pfeuty T., Changeux J. P. Regulation of binding properties of the nicotinic receptor protein by cholinergic ligands in membrane fragments from Torpedo marmorata. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3443–3447. doi: 10.1073/pnas.72.9.3443. [DOI] [PMC free article] [PubMed] [Google Scholar]

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