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. 1980 Apr;301:25–37. doi: 10.1113/jphysiol.1980.sp013185

Stimulation of the sodium-potassium pump by trypsin in low potassium type erythrocytes of goats.

P B Dunham, J C Ellory
PMCID: PMC1279378  PMID: 7411431

Abstract

1. Treatment of low K goat red cells with trypsin stimulated the Na-K pump more than twofold. Dose dependence and time course experiments indicated a half-maximal stimulation at 1.6 mg trypsin/ml. (37 degrees C, 3 hr), and a maximum effect after 5 hr (10 mg/ml). 2. Trypsin had only a small and variable effect on the ouabain-insensitive component of K influx. 3. The Na-K pump activity of high K goat red cells was not affected by trypsinization. 4. When intracellular K was varied by the PCMBS technique, it was found that the trypsin stimulation was greatest (2-5-fold) in cells with the highest K (40 m-mole/1. cells) and lowest (1.1-fold) in cells with low K (<1 m-mole/1. cells). 5. The trypsin effect was reversed by nystatin treatment or hypotonic lysis. 6. Trypsin did not increase the number of ouabain-binding sites. 7. It is concluded that trypsinization modifies the L antigen in low K goat red cells to decrease the apparent internal affinity for K of the Na-K pump in these cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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