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. 1980 Apr;301:365–382. doi: 10.1113/jphysiol.1980.sp013211

Arousal of a specific and persistent sodium appetite in the rat with continuous intracerebroventricular infusion of angiotensin II.

R W Bryant, A N Epstein, J T Fitzsimons, S J Fluharty
PMCID: PMC1279404  PMID: 7411437

Abstract

1. Prolonged exposure of the brain of the normal Na-replete rat to angiotensin II produced a marked and persistent Na appetite. In a first series of experiments, short-term, repeated systemic injections of isoprenaline or renin (both of which raise circulating angiotensin levels), and repeated intracranial injections of angiotensin II evoked increased ingestion of 2 . 7% NaCl. In the second series of experiments, continuous infusions of angiotensin II directly into the brain evoked extremely large intakes of 3% NaCl. 2. In addition to large intakes of hypertonic NaCl some rats drank daily volumes of water that exceeded their body weight. 3. Not only did the animals drink large volumes of 3% NaCl some rats drank daily volumes of water that exceeded their body weight. 3. Not only did the animals drink large volumes of 3% NaCl during continuous angiotensin II infusion, but after termination of the infusion they continued to ingest NaCl at a rate comparable to that of the adrenalectomized rat. In most of the animals the persistent NaCl intake diminished over several days, but other animals continued to drink NaCl for as long as their intake was measured (up to 7 months). 4. The response to continuous infusion of angiotensin II was dose-dependent. Both water and 3% NaCl intake increased over a dose range of 6 ng h-1 to 6000 ng h-1. The persistence of the sodium appetitite was also dose-dependent across the same range of doses. 5. Angiotensin-induced salt appetite is specific for Na. Animals did not drink 0 . 5 M-NH4Cl and only occasionally drank minimal amounts of 0 . 5 M-KCl during continuous infusion. 6. The large water turnover was not responsible for the Na appetite. Rats given access to 3% NaCl only during infusion of angiotensin copiously. Animals that were not infused but were given saccharine-flavoured water in order to increase their water intakes did not drink 3% NaCl offered at the same time even though fluid intake was high. Rats that did not receive intracranial infusions but were infused intragastrically with volumes of water equal to or exceeding the amounts that were drunk during angiotensin infusion did not drink the 3% NaCl but did drink some water. 7. Records of the drinking by rats infused with angiotensin show that firstly the onset of drinking after the start of angiotensin infusion varied from animal to animal, secondly, NaCl drinking was not temporally linked to water intake, although this was observed occasionally, and thirdly, most of the drinking occurred during the night although angiotensin was infused continuously throughtout the nychthemeron. 8. Therefore, increases in angiotensin levels, probably with other factors such as increased levels of aldosterone or ACTH, result in Na appetite. The hormonal changes may alter the animals' preception of salt making it more acceptable. By means that are not yet understood the increased accceptability of salt persists after the termination of angiotensin infusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrith D., Fitzsimons J. T. Intracranial angiotensin II stimulates sodium appetite in the rat [proceedings]. J Physiol. 1978 Sep;282:40P–41P. [PubMed] [Google Scholar]
  2. BEILHARZ S., DENTON D. A., SABINE J. R. The effect of concurrent deficiency of water and sodium on the sodium appetite of sheep. J Physiol. 1962 Oct;163:378–390. doi: 10.1113/jphysiol.1962.sp006983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bott E., Denton D. A., Weller S. The effect of angiotensin II infusion, renal hypertension and nephrectomy on salt appetite of sodium-deficient sheep. Aust J Exp Biol Med Sci. 1967 Dec;45(6):595–612. doi: 10.1038/icb.1967.61. [DOI] [PubMed] [Google Scholar]
  4. Buggy J., Fisher A. E. Evidence for a dual central role for angiotensin in water and sodium intake. Nature. 1974 Aug 30;250(5469):733–735. doi: 10.1038/250733a0. [DOI] [PubMed] [Google Scholar]
  5. Chiaraviglio E. Effect of renin-angiotensin system on sodium intake. J Physiol. 1976 Feb;255(1):57–66. doi: 10.1113/jphysiol.1976.sp011269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Covian M. R., Antunes-Rodrigues J. Specific alterations in sodium chloride intake after hypothalamic lesions in the rat. Am J Physiol. 1963 Nov;205(5):922–926. doi: 10.1152/ajplegacy.1963.205.5.922. [DOI] [PubMed] [Google Scholar]
  7. DENTON D. A., SABINE J. R. The selective appetite for Na ions shown by Na ion-deficient sheep. J Physiol. 1961 Jun;157:97–116. doi: 10.1113/jphysiol.1961.sp006708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. EPSTEIN A. N., STELLAR E. The control of salt preference in the adrenalectomized rat. J Comp Physiol Psychol. 1955 Jun;48(3):167–172. doi: 10.1037/h0045626. [DOI] [PubMed] [Google Scholar]
  9. EPSTEIN A. N., TEITELBAUM P. A watertight swivel joint permitting chronic injection into moving animals. J Appl Physiol. 1962 Jan;17:171–172. doi: 10.1152/jappl.1962.17.1.171. [DOI] [PubMed] [Google Scholar]
  10. Epstein A. N., Fitzsimons J. T., Rolls B. J. Drinking induced by injection of angiotensin into the rain of the rat. J Physiol. 1970 Sep;210(2):457–474. doi: 10.1113/jphysiol.1970.sp009220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FITZSIMONS J. T. DRINKING CAUSED BY CONSTRICTION OF THE INFERIOR VENA CAVA IN THE RAT. Nature. 1964 Oct 31;204:479–480. doi: 10.1038/204479a0. [DOI] [PubMed] [Google Scholar]
  12. Falk J. L. Limitations to the specificity of NaCl appetite in sodium-depleted rats. J Comp Physiol Psychol. 1965 Dec;60(3):393–396. doi: 10.1037/h0022559. [DOI] [PubMed] [Google Scholar]
  13. Fitzsimons J. T., Wirth J. B. The renin-angiotensin system and sodium appetite. J Physiol. 1978 Jan;274:63–80. doi: 10.1113/jphysiol.1978.sp012134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. NACHMAN M. Taste preferences for sodium salts by adrenalectomized rats. J Comp Physiol Psychol. 1962 Dec;55:1124–1129. doi: 10.1037/h0041348. [DOI] [PubMed] [Google Scholar]
  15. Nachman M., Valentino D. A. Roles of taste and postingestional factors in the satiation of sodium appetite in rats. J Comp Physiol Psychol. 1966 Oct;62(2):280–283. doi: 10.1037/h0023667. [DOI] [PubMed] [Google Scholar]
  16. Peskar B., Meyer D. K., Tauchmann U., Hertting G. Influence of isoproterenol, hydralazine and phentolamine on the renin activity of plasma and renal cortex of rats. Eur J Pharmacol. 1970 Mar;9(3):394–396. doi: 10.1016/0014-2999(70)90243-8. [DOI] [PubMed] [Google Scholar]
  17. Radio G. J., Summy-Long J., Daniels-Severs A., Severs W. B. Hydration changes produced by central infusion of angiotensin II. Am J Physiol. 1972 Nov;223(5):1221–1226. doi: 10.1152/ajplegacy.1972.223.5.1221. [DOI] [PubMed] [Google Scholar]
  18. Wolf G., Handal P. J. Aldosterone-induced sodium appetite: dose-response and specificity. Endocrinology. 1966 Jun;78(6):1120–1124. doi: 10.1210/endo-78-6-1120. [DOI] [PubMed] [Google Scholar]
  19. Wolf G. Hypothalamic regulation of sodium intake: relations to preoptic and tegmental function. Am J Physiol. 1967 Dec;213(6):1433–1438. doi: 10.1152/ajplegacy.1967.213.6.1433. [DOI] [PubMed] [Google Scholar]
  20. Wolf G. Neural mechanisms for sodium appetite: hypothalamus positive--hypothalamofugal pathways negative. Physiol Behav. 1971 Apr;6(4):381–389. doi: 10.1016/0031-9384(71)90171-5. [DOI] [PubMed] [Google Scholar]

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