Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1989 Aug;56(2):327–337. doi: 10.1016/S0006-3495(89)82679-7

Location of the heme-Fe atoms within the profile structure of a monolayer of cytochrome c bound to the surface of an ultrathin lipid multilayer film.

J M Pachence 1, R F Fischetti 1, J K Blasie 1
PMCID: PMC1280482  PMID: 2550089

Abstract

We have recently developed x-ray diffraction methods to derive the profile structure of ultrathin lipid multilayer films having one to five bilayers (e.g., Skita, V., W. Richardson, M. Filipkowski, A.F. Garito, and J.K. Blasie. 1987. J. Physique. 47:1849-1855). Furthermore, we have employed these techniques to determine the location of a monolayer of cytochrome c bound to the carboxyl group surface of various ultrathin lipid multilayer substrates via nonresonance x-ray diffraction (Pachence, J.M., and J.K. Blasie. 1987. Biophys. J. 52:735-747). Here an intense tunable source of x-rays (beam line X9-A at the National Synchrotron Light Source at the Brookhaven National Laboratory) was utilized to measure the resonance x-ray diffraction effect from the heme-Fe atoms within the cytochrome c molecular monolayer located on the carboxyl surface of a five monolayer arachidic acid film. Lamellar x-ray diffraction was recorded for energies above, below, and at the Fe K-absorption edge (E = 7,112 eV). An analysis of the resonance x-ray diffraction effect is presented, whereby the location of the heme-Fe atoms within the electron density profile of the cytochrome c/arachidic acid ultrathin multilayer film is indicated to +/- 3 A accuracy.

Full text

PDF
327

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blasie J. K., Erecińska M., Samuels S., Leigh J. S. The structure of a cytochrome oxidase-lipid model membrane. Biochim Biophys Acta. 1978 Jan 11;501(1):33–52. doi: 10.1016/0005-2728(78)90093-2. [DOI] [PubMed] [Google Scholar]
  2. Blasie J. K., Pachence J. M., Tavormina A., Erecinska M., Dutton P. L., Stamatoff J., Eisenberger P., Brown G. The location of redox centers in biological membranes determined by resonance x-ray diffraction. II. Analysis of the resonance diffraction data. Biochim Biophys Acta. 1982 Feb 17;679(2):188–197. doi: 10.1016/0005-2728(82)90290-0. [DOI] [PubMed] [Google Scholar]
  3. Blasie J. K., Stamatoff J. Resonance X-ray scattering: its use in determining spatial relationships among metal atoms within macromolecules in a non-crystalline state. Annu Rev Biophys Bioeng. 1981;10:451–458. doi: 10.1146/annurev.bb.10.060181.002315. [DOI] [PubMed] [Google Scholar]
  4. Fischetti RF, Filipkowski M, Garito AF, Blasie JK. Profile structures of ultrathin periodic and nonperiodic multilayer films containing a disubstituted diacetylene by high-resolution x-ray diffraction. Phys Rev B Condens Matter. 1988 Mar 15;37(9):4714–4726. doi: 10.1103/physrevb.37.4714. [DOI] [PubMed] [Google Scholar]
  5. Fischetti RF, Skita V, V, Garito AF, Blasie JK. Asymmetry in the interior arachidic-acid bilayers within ultrathin multilayers fabricated via the Langmuir-Blodgett technique. Phys Rev B Condens Matter. 1988 Mar 15;37(9):4788–4791. doi: 10.1103/physrevb.37.4788. [DOI] [PubMed] [Google Scholar]
  6. Henderson R., Unwin P. N. Three-dimensional model of purple membrane obtained by electron microscopy. Nature. 1975 Sep 4;257(5521):28–32. doi: 10.1038/257028a0. [DOI] [PubMed] [Google Scholar]
  7. Pachence J. M., Blasie J. K. The location of cytochrome c on the surface of ultrathin lipid multilayer films using x-ray diffraction. Biophys J. 1987 Nov;52(5):735–747. doi: 10.1016/S0006-3495(87)83268-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pachence J. M., Dutton P. L., Blasie J. K. A structural investigation of cytochrome c binding to photosynthetic reaction centers in reconstituted membranes. Biochim Biophys Acta. 1983 Jul 29;724(1):6–19. doi: 10.1016/0005-2728(83)90021-x. [DOI] [PubMed] [Google Scholar]
  9. Pachence J. M., Dutton P. L., Blasie J. K. Structural studies on reconstituted reaction center-phosphatidylcholine membranes. Biochim Biophys Acta. 1979 Nov 8;548(2):348–373. doi: 10.1016/0005-2728(79)90141-5. [DOI] [PubMed] [Google Scholar]
  10. Pachence J. M., Dutton P. L., Blasie J. K. The reaction center profile structure derived from neutron diffraction. Biochim Biophys Acta. 1981 Apr 13;635(2):267–283. doi: 10.1016/0005-2728(81)90026-8. [DOI] [PubMed] [Google Scholar]
  11. Skita V, V, Filipkowski M, Garito AF, Blasie JK. Profile structures of very thin multilayers by x-ray diffraction using direct and refinement methods of analysis. Phys Rev B Condens Matter. 1986 Oct 15;34(8):5826–5837. doi: 10.1103/physrevb.34.5826. [DOI] [PubMed] [Google Scholar]
  12. Stamatoff J., Eisenberger P., Blasie J. K., Pachence J. M., Tavormina A., Erecinska M., Dutton P. L., Brown G. The location of redox centers in biological membranes determined by resonance x-ray diffraction. I. Observation of the resonance effect. Biochim Biophys Acta. 1982 Feb 17;679(2):177–187. doi: 10.1016/0005-2728(82)90289-4. [DOI] [PubMed] [Google Scholar]
  13. Stroud R. M., Agard D. A. Structure determination of asymmetric membrane profiles using an iterative Fourier method. Biophys J. 1979 Mar;25(3):495–512. doi: 10.1016/S0006-3495(79)85319-9. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES