Skip to main content
PMC home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1979 Sep;294:123–134. doi: 10.1113/jphysiol.1979.sp012919

Possible mechanism of the inhibitory effect of ouabain on renin secretion from rat renal cortical slices.

P C Churchill
PMCID: PMC1280546  PMID: 117099

Abstract

1. The effects of ouabain on renin secretion by rat renal cortical slices were studied. 2. Renin secretion was inhibited by 10(-3) M-ouabain in the presence of free Ca (10(-4) to 2.6 x 10(-3) M). Inhibition was blocked at Ca less than 10(-8) M. 3. The effect of free Ca on ouabain-inhibition was shown to be independent of the presence of EGTA, completely reversible, and unrelated to passive leakage of renin from non-viable cells, as assessed by simultaneous release of lactate dehydrogenase activity (LDH). 4. It is proposed that, as a result of inhibition of Na, K-ATPase by ouabain, (a) intracellular Na increases in the renin-secreting juxtaglomerular cells, (b) intracellular Ca increases, via an Na-Ca exchange mechanism, and (c) that Ca accumulation, in some unknown manner, inhibits renin secretion.

Full text

PDF
123

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akera T., Brody T. M. The role of Na+,K+-ATPase in the inotropic action of digitalis. Pharmacol Rev. 1977 Sep;29(3):187–220. [PubMed] [Google Scholar]
  2. Aoi W., Wade M. B., Rosner D. R., Weinberger M. H. Renin release by rat kidney slices in vitro: effects of cations and catecholamines. Am J Physiol. 1974 Sep;227(3):630–634. doi: 10.1152/ajplegacy.1974.227.3.630. [DOI] [PubMed] [Google Scholar]
  3. Banks P. The effect of ouabain on the secretion of catecholamines and on the intracellular concentration of potassium. J Physiol. 1967 Dec;193(3):631–637. doi: 10.1113/jphysiol.1967.sp008383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baumbach L., Leyssac P. P. Studies on the mechanism of renin release from isolated superfused rat glomeruli: effects of calcium, calcium ionophore and lanthanum. J Physiol. 1977 Dec;273(3):745–764. doi: 10.1113/jphysiol.1977.sp012121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Birks R. I., Cohen M. W. The influence of internal sodium on the behaviour of motor nerve endings. Proc R Soc Lond B Biol Sci. 1968 Jul 9;170(1021):401–421. doi: 10.1098/rspb.1968.0047. [DOI] [PubMed] [Google Scholar]
  6. Blaine E. H., Zimmerman M. B. Renal function and renin secretion after administration of ouabain and ouabain plus furosemide in conscious sheep. Circ Res. 1978 Jul;43(1):36–43. doi: 10.1161/01.res.43.1.36. [DOI] [PubMed] [Google Scholar]
  7. Blaustein M. P. The interrelationship between sodium and calcium fluxes across cell membranes. Rev Physiol Biochem Pharmacol. 1974;70:33–82. doi: 10.1007/BFb0034293. [DOI] [PubMed] [Google Scholar]
  8. Blendstrup K., Leyssac P. P., Poulsen K., Skinner S. L. Characteristics of renin release from isolated superfused glomeruli in vitro. J Physiol. 1975 Apr;246(3):653–672. doi: 10.1113/jphysiol.1975.sp010909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bohr D. F. Vascular smooth muscle updated. Circ Res. 1973 Jun;32(6):665–672. doi: 10.1161/01.res.32.6.665. [DOI] [PubMed] [Google Scholar]
  10. Braverman B., Freeman R. H., Rostorfer H. H. The influence of dietary sodium chloride on in vitro renin release from rat kidney slices. Proc Soc Exp Biol Med. 1971 Oct;138(1):81–88. doi: 10.3181/00379727-138-35836. [DOI] [PubMed] [Google Scholar]
  11. Churchill P. C., Churchill M. C., McDonald F. D. Renin secretion and distal tubule Na+ in rats. Am J Physiol. 1978 Dec;235(6):F611–F616. doi: 10.1152/ajprenal.1978.235.6.F611. [DOI] [PubMed] [Google Scholar]
  12. Churchill P. C., McDonald F. D. Effect of ouabain on renin secretion in anaesthetized dogs. J Physiol. 1974 Nov;242(3):635–646. doi: 10.1113/jphysiol.1974.sp010727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Churchill P., Bidani A., Fleischmann L., Becker-McKenna B. Glycerol-induced acute renal failure in the two kidney Goldblatt rat. Am J Physiol. 1977 Sep;233(3):F247–F252. doi: 10.1152/ajprenal.1977.233.3.F247. [DOI] [PubMed] [Google Scholar]
  14. Corsini W. A., Crosslan K. L., Bailie M. D. Renin secretion by rat kidney slices in vitro. Proc Soc Exp Biol Med. 1974 Feb;145(2):403–406. doi: 10.3181/00379727-145-37819. [DOI] [PubMed] [Google Scholar]
  15. De Vito E., Gordon S. B., Cabrera R. R., Fasciolo J. C. Release of renin by rat kidney slices. Am J Physiol. 1970 Oct;219(4):1036–1041. doi: 10.1152/ajplegacy.1970.219.4.1036. [DOI] [PubMed] [Google Scholar]
  16. Dicker S. E. Release of vasopressin and oxytocin from isolated pituitary glands of adult and new-born rats. J Physiol. 1966 Jul;185(2):429–444. doi: 10.1113/jphysiol.1966.sp007994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Eto S., Wood J. M., Hutchins M., Fleischer N. Pituitary 45 Ca ion uptake and release of ACTH, GH, and TSH: effect of verapamil. Am J Physiol. 1974 Jun;226(6):1315–1320. doi: 10.1152/ajplegacy.1974.226.6.1315. [DOI] [PubMed] [Google Scholar]
  18. Forgács I., Gaál K. Effect of sodium and osmotic concentration on renin release by renal cortical slices in vitro. Acta Physiol Acad Sci Hung. 1971;40(2):139–144. [PubMed] [Google Scholar]
  19. Fynn M., Onomakpome N., Peart W. S. The effects of ionophores (A23187 and RO2-2985) on renin secretion and renal vasoconstriction. Proc R Soc Lond B Biol Sci. 1977 Nov 14;199(1135):199–212. doi: 10.1098/rspb.1977.0135. [DOI] [PubMed] [Google Scholar]
  20. Grodsky G. M. Insulin and the pancreas. Vitam Horm. 1970;28:37–101. doi: 10.1016/s0083-6729(08)60888-2. [DOI] [PubMed] [Google Scholar]
  21. Hales C. N., Milner R. D. Cations and the secretion of insulin from rabbit pancreas in vitro. J Physiol. 1968 Nov;199(1):177–187. doi: 10.1113/jphysiol.1968.sp008647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Hales C. N., Milner R. D. The role of sodium and potassium in insulin secretion from rabbit pancreas. J Physiol. 1968 Feb;194(3):725–743. doi: 10.1113/jphysiol.1968.sp008433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Haulica I., Petrescu G., Branisteanu D., Rosca V., Balan G. Recherches sur le rôle des phénomènes membranaires dans la sécrétion de la rénine. J Physiol (Paris) 1974 Mar;68(1):17–26. [PubMed] [Google Scholar]
  24. Hughes P. M., Macknight D. C. The regulation of cellular volume in renal cortical slices incubated in hyposmotic medium. J Physiol. 1976 May;257(1):137–154. doi: 10.1113/jphysiol.1976.sp011360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Kelly J. S. The antagonism of Ca2+ by Na+ and other monovalent ions at the frog neuromuscular junction. Q J Exp Physiol Cogn Med Sci. 1968 Jul;53(3):239–249. doi: 10.1113/expphysiol.1968.sp001967. [DOI] [PubMed] [Google Scholar]
  26. Lee K. S., Klaus W. The subcellular basis for the mechanism of inotropic action of cardiac glycosides. Pharmacol Rev. 1971 Sep;23(3):193–261. [PubMed] [Google Scholar]
  27. Lyons H. J., Churchill P. C. The influence of ouabain on in vitro renin secretion and intracellular sodium. Nephron. 1975;14(6):442–450. doi: 10.1159/000180478. [DOI] [PubMed] [Google Scholar]
  28. Lyons H. J., Churchill P. C. The influence of ouabain on in vitro renin secretion. Proc Soc Exp Biol Med. 1974 Apr;145(4):1148–1150. doi: 10.3181/00379727-145-37970. [DOI] [PubMed] [Google Scholar]
  29. Maude D. L. Effects of K and ouabain on fluid transport and cell Na in proximal tubule in vitro. Am J Physiol. 1969 May;216(5):1199–1206. doi: 10.1152/ajplegacy.1969.216.5.1199. [DOI] [PubMed] [Google Scholar]
  30. Michelakis A. M. The effect of sodium and calcium on renin release in vitro. Proc Soc Exp Biol Med. 1971 Jul;137(3):833–836. doi: 10.3181/00379727-137-35677. [DOI] [PubMed] [Google Scholar]
  31. Park C. S., Malvin R. L. Calcium in the control of renin release. Am J Physiol. 1978 Jul;235(1):F22–F25. doi: 10.1152/ajprenal.1978.235.1.F22. [DOI] [PubMed] [Google Scholar]
  32. Park C. S., Malvin R. L., Murray R. D., Cho K. W. Renin secretion as a function of renal renin content in dogs. Am J Physiol. 1978 Jun;234(6):F506–F509. doi: 10.1152/ajprenal.1978.234.6.F506. [DOI] [PubMed] [Google Scholar]
  33. Van Dongen R., Peart W. S. Calcium dependence of the inhibitory effect of angiotensin on renin secretion in the isolated perfused kidney of the rat. Br J Pharmacol. 1974 Jan;50(1):125–129. doi: 10.1111/j.1476-5381.1974.tb09599.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Vander A. J. Direct effects of potassium on renin secretion and renal function. Am J Physiol. 1970 Aug;219(2):455–459. doi: 10.1152/ajplegacy.1970.219.2.455. [DOI] [PubMed] [Google Scholar]
  35. Vander A. J. Effect of catecholamines and the renal nerves on renin secretion in anesthetized dogs. Am J Physiol. 1965 Sep;209(3):659–662. doi: 10.1152/ajplegacy.1965.209.3.659. [DOI] [PubMed] [Google Scholar]
  36. Vander A. J., Geelhoed G. W. Inhibition of renin secretion by angiotensin. II. Proc Soc Exp Biol Med. 1965 Nov;120(2):399–403. doi: 10.3181/00379727-120-30547. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES