Skip to main content
NCBI home page
Biophysical Journal logoLink to Biophysical Journal
. 1989 Nov;56(5):845–850. doi: 10.1016/S0006-3495(89)82730-4

Effect of cholesterol on structural and dynamic properties of tripalmitoyl glyceride. A high-pressure infrared spectroscopic study.

P T Wong 1, T E Chagwedera 1, H H Mantsch 1
PMCID: PMC1280583  PMID: 2605300

Abstract

The infrared spectra of tripalmitoyl glyceride confirm the tuning fork configuration previously attributed to trilauroyl glyceride (Small, D. M. 1986. Handbook of Lipid Research. Vol. 4). The acyl chains in solid tripalmitoyl glycerol, either within each molecule or between neighboring molecules, are oriented parallel to each other with the sn-3 acyl chains extended toward the opposite direction of the sn-1 and sn-2 chains. The presence of cholesterol increases the orientational disorder of the tripalmitoyl glyceride molecules in terms of increased reorientational fluctuations and twisting/torsion motions of the acyl chains. In the solid mixture, cholesterol is embedded in the tripalmitoyl glyceride lattice which results in a reorientation of the acyl chains within each molecule from a parallel packing to a nonparallel packing. No evidence was found for hydrogen bond formation between the OH group of cholesterol and any of the three C = O groups of tripalmitoyl glyceride.

Full text

PDF
845

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auger M., Jarrell H. C., Smith I. C., Siminovitch D. J., Mantsch H. H., Wong P. T. Effects of the local anesthetic tetracaine on the structural and dynamic properties of lipids in model membranes: a high-pressure Fourier transform infrared study. Biochemistry. 1988 Aug 9;27(16):6086–6093. doi: 10.1021/bi00416a038. [DOI] [PubMed] [Google Scholar]
  2. Auger M., Jarrell H. C., Smith I. C., Wong P. T., Siminovitch D. J., Mantsch H. H. Pressure-induced exclusion of a local anesthetic from model and nerve membranes. Biochemistry. 1987 Dec 29;26(26):8513–8516. doi: 10.1021/bi00400a003. [DOI] [PubMed] [Google Scholar]
  3. Brindley D. N. What factors control hepatic triacylglycerol accumulation in alcohol abuse? Biochem Soc Trans. 1988 Jun;16(3):251–253. doi: 10.1042/bst0160251. [DOI] [PubMed] [Google Scholar]
  4. Brooks C. J., Harland W. A., Steel G. Squalene, 26-hydroxycholesterol and 7-ketocholesterol in human atheromatous plaques. Biochim Biophys Acta. 1966 Dec 7;125(3):620–622. doi: 10.1016/0005-2760(66)90055-5. [DOI] [PubMed] [Google Scholar]
  5. French S. W. Biochemical basis for alcohol-induced liver injury. Clin Biochem. 1989 Feb;22(1):41–49. doi: 10.1016/s0009-9120(89)80067-0. [DOI] [PubMed] [Google Scholar]
  6. Gao Q., Craven B. M. Conformation of the oleate chains in crystals of cholesteryl oleate at 123 K. J Lipid Res. 1986 Nov;27(11):1214–1221. [PubMed] [Google Scholar]
  7. Mushayakarara E. C., Wong P. T., Mantsch H. H. Detection by high pressure infrared spectrometry of hydrogen-bonding between water and triacetyl glycerol. Biochem Biophys Res Commun. 1986 Jan 14;134(1):140–145. doi: 10.1016/0006-291x(86)90538-3. [DOI] [PubMed] [Google Scholar]
  8. Parker F. S., Bhaskar K. R. Self-association of cholesterol and its interaction with triglycerides. An infrared study. Biochemistry. 1968 Apr;7(4):1286–1290. doi: 10.1021/bi00844a006. [DOI] [PubMed] [Google Scholar]
  9. Wong P. T., Capes S. E., Mantsch H. H. Hydrogen bonding between anhydrous cholesterol and phosphatidylcholines: an infrared spectroscopic study. Biochim Biophys Acta. 1989 Mar 27;980(1):37–41. doi: 10.1016/0005-2736(89)90197-1. [DOI] [PubMed] [Google Scholar]
  10. Wong P. T., Mantsch H. H. Reorientational and conformational ordering processes at elevated pressures in 1,2-dioleoyl phosphatidylcholine: a Raman and infrared spectroscopic study. Biophys J. 1988 Nov;54(5):781–790. doi: 10.1016/S0006-3495(88)83016-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wong P. T. Raman spectroscopy of thermotropic and high-pressure phases of aqueous phospholipid dispersions. Annu Rev Biophys Bioeng. 1984;13:1–24. doi: 10.1146/annurev.bb.13.060184.000245. [DOI] [PubMed] [Google Scholar]
  12. Wong P. T., Siminovitch D. J., Mantsch H. H. Structure and properties of model membranes: new knowledge from high-pressure vibrational spectroscopy. Biochim Biophys Acta. 1988 Feb 24;947(1):139–171. doi: 10.1016/0304-4157(88)90023-8. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES