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. 1990 Jan;57(1):23–31. doi: 10.1016/S0006-3495(90)82503-0

Interaction between the VDAC channel and a polyanionic effector. An electron microscopic study.

C A Mannella 1, X W Guo 1
PMCID: PMC1280639  PMID: 1688715

Abstract

The conductance of the voltage-dependent mitochondrial outer membrane channel is modulated by a synthetic anionic polymer. When added to suspensions of membrane crystals of the channel, the polyanion caused disordering of the usual parallelogram array and increased occurrence of a contracted form of the array. Correlation averages obtained from electron microscopic images of the channel crystals indicated a narrowing of the projected channel lumen in the presence of the polyanion and the appearance of new, narrow zones of stain exclusion on the outside of the channel. These effects are interpreted in terms of possible conformational changes induced in the channel by binding of the polyanion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amos L. A., Henderson R., Unwin P. N. Three-dimensional structure determination by electron microscopy of two-dimensional crystals. Prog Biophys Mol Biol. 1982;39(3):183–231. doi: 10.1016/0079-6107(83)90017-2. [DOI] [PubMed] [Google Scholar]
  2. Benz R. Porin from bacterial and mitochondrial outer membranes. CRC Crit Rev Biochem. 1985;19(2):145–190. doi: 10.3109/10409238509082542. [DOI] [PubMed] [Google Scholar]
  3. Benz R., Wojtczak L., Bosch W., Brdiczka D. Inhibition of adenine nucleotide transport through the mitochondrial porin by a synthetic polyanion. FEBS Lett. 1988 Apr 11;231(1):75–80. doi: 10.1016/0014-5793(88)80706-3. [DOI] [PubMed] [Google Scholar]
  4. Colombini M. A candidate for the permeability pathway of the outer mitochondrial membrane. Nature. 1979 Jun 14;279(5714):643–645. doi: 10.1038/279643a0. [DOI] [PubMed] [Google Scholar]
  5. Colombini M. Structure and mode of action of a voltage dependent anion-selective channel (VDAC) located in the outer mitochondrial membrane. Ann N Y Acad Sci. 1980;341:552–563. doi: 10.1111/j.1749-6632.1980.tb47198.x. [DOI] [PubMed] [Google Scholar]
  6. Colombini M., Yeung C. L., Tung J., König T. The mitochondrial outer membrane channel, VDAC, is regulated by a synthetic polyanion. Biochim Biophys Acta. 1987 Dec 11;905(2):279–286. doi: 10.1016/0005-2736(87)90456-1. [DOI] [PubMed] [Google Scholar]
  7. De Pinto V., Ludwig O., Krause J., Benz R., Palmieri F. Porin pores of mitochondrial outer membranes from high and low eukaryotic cells: biochemical and biophysical characterization. Biochim Biophys Acta. 1987 Nov 19;894(2):109–119. doi: 10.1016/0005-2728(87)90180-0. [DOI] [PubMed] [Google Scholar]
  8. Frank J., Verschoor A., Boublik M. Multivariate statistical analysis of ribosome electron micrographs. L and R lateral views of the 40 S subunit from HeLa cells. J Mol Biol. 1982 Oct 15;161(1):107–133. doi: 10.1016/0022-2836(82)90281-9. [DOI] [PubMed] [Google Scholar]
  9. Holden M. J., Colombini M. The mitochondrial outer membrane channel, VDAC, is modulated by a soluble protein. FEBS Lett. 1988 Dec 5;241(1-2):105–109. doi: 10.1016/0014-5793(88)81040-8. [DOI] [PubMed] [Google Scholar]
  10. Kessel M., Radermacher M., Frank J. The structure of the stalk surface layer of a brine pond microorganism: correlation averaging applied to a double layered lattice structure. J Microsc. 1985 Jul;139(Pt 1):63–74. doi: 10.1111/j.1365-2818.1985.tb04662.x. [DOI] [PubMed] [Google Scholar]
  11. Kühlbrandt W. Discrimination of protein and nucleic acids by electron microscopy using contrast variation. Ultramicroscopy. 1982;7(3):221–232. doi: 10.1016/0304-3991(82)90169-3. [DOI] [PubMed] [Google Scholar]
  12. Mannella C. A., Colombini M., Frank J. Structural and functional evidence for multiple channel complexes in the outer membrane of Neurospora crassa mitochondria. Proc Natl Acad Sci U S A. 1983 Apr;80(8):2243–2247. doi: 10.1073/pnas.80.8.2243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mannella C. A., Frank J. Negative staining characteristics of arrays of mitochondrial pore protein: use of correspondence analysis to classify different staining patterns. Ultramicroscopy. 1984;13(1-2):93–102. doi: 10.1016/0304-3991(84)90060-3. [DOI] [PubMed] [Google Scholar]
  14. Mannella C. A. Mitochondrial outer membrane channel (VDAC, porin) two-dimensional crystals from Neurospora. Methods Enzymol. 1986;125:595–610. doi: 10.1016/s0076-6879(86)25048-x. [DOI] [PubMed] [Google Scholar]
  15. Mannella C. A. Phospholipase-induced crystallization of channels in mitochondrial outer membranes. Science. 1984 Apr 13;224(4645):165–166. doi: 10.1126/science.6322311. [DOI] [PubMed] [Google Scholar]
  16. Mannella C. A., Ribeiro A. J., Frank J. Cytochrome c binds to lipid domains in arrays of mitochondrial outer membrane channels. Biophys J. 1987 Feb;51(2):221–226. doi: 10.1016/S0006-3495(87)83327-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mannella C. A., Ribeiro A., Frank J. Structure of the Channels in the Outer Mitochondrial Membrane: Electron Microscopic Studies of the Periodic Arrays Induced by Phospholipase a(2) Treatment of the Neurospora membrane. Biophys J. 1986 Jan;49(1):307–317. doi: 10.1016/s0006-3495(86)83643-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mannella C. A. Structure of the outer mitochondrial membrane: ordered arrays of porelike subunits in outer-membrane fractions from Neurospora crassa mitochondria. J Cell Biol. 1982 Sep;94(3):680–687. doi: 10.1083/jcb.94.3.680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Saxton W. O., Baumeister W. The correlation averaging of a regularly arranged bacterial cell envelope protein. J Microsc. 1982 Aug;127(Pt 2):127–138. doi: 10.1111/j.1365-2818.1982.tb00405.x. [DOI] [PubMed] [Google Scholar]
  20. Tedeschi H., Mannella C. A., Bowman C. L. Patch clamping the outer mitochondrial membrane. J Membr Biol. 1987;97(1):21–29. doi: 10.1007/BF01869611. [DOI] [PubMed] [Google Scholar]
  21. Zalman L. S., Nikaido H., Kagawa Y. Mitochondrial outer membrane contains a protein producing nonspecific diffusion channels. J Biol Chem. 1980 Mar 10;255(5):1771–1774. [PubMed] [Google Scholar]

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