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. 1990 Jan;57(1):63–69. doi: 10.1016/S0006-3495(90)82507-8

Kinetics of channel formation of gramicidins A and B in phospholipid vesicle membranes.

P L Easton 1, J F Hinton 1, D K Newkirk 1
PMCID: PMC1280643  PMID: 1688716

Abstract

The thermal incorporation and channel formation of gramicidins A and B into phosphatidylcholine/phosphatidylglycerol large unilamellar vesicle membranes was studied using 23Na NMR. Delta H and delta S of activation for channel formation for gramicidin A are 11.8 kcal/mol and -11 e.u., respectively. For gramicidin B, delta H and delta S of activation are 14.6 kcal/mol and -4 e.u., respectively. Possible reasons for the differences in delta H and delta S of activation between the two analogues are discussed.

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Selected References

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  1. Andersen O. S. Ion movement through gramicidin A channels. Single-channel measurements at very high potentials. Biophys J. 1983 Feb;41(2):119–133. doi: 10.1016/S0006-3495(83)84414-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bamberg E., Apell H. J., Alpes H. Structure of the gramicidin A channel: discrimination between the piL,D and the beta helix by electrical measurements with lipid bilayer membranes. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2402–2406. doi: 10.1073/pnas.74.6.2402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bamberg E., Läuger P. Channel formation kinetics of gramicidin A in lipid bilayer membranes. J Membr Biol. 1973;11(2):177–194. doi: 10.1007/BF01869820. [DOI] [PubMed] [Google Scholar]
  4. Bamberg E., Noda K., Gross E., Läuger P. Single-channel parameters of gramicidin A,B, and C. Biochim Biophys Acta. 1976 Jan 21;419(2):223–228. doi: 10.1016/0005-2736(76)90348-5. [DOI] [PubMed] [Google Scholar]
  5. Buster D. C., Hinton J. F., Millett F. S., Shungu D. C. 23Na-nuclear magnetic resonance investigation of gramicidin-induced ion transport through membranes under equilibrium conditions. Biophys J. 1988 Feb;53(2):145–152. doi: 10.1016/S0006-3495(88)83076-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cavatorta P., Spisni A., Casali E., Lindner L., Masotti L., Urry D. W. Intermolecular interactions of gramicidin A' transmembrane channels incorporated into lysophosphatidylcholine lipid systems. Biochim Biophys Acta. 1982 Jul 14;689(1):113–120. doi: 10.1016/0005-2736(82)90195-x. [DOI] [PubMed] [Google Scholar]
  7. Cullis P. R., de Kruijff B. Lipid polymorphism and the functional roles of lipids in biological membranes. Biochim Biophys Acta. 1979 Dec 20;559(4):399–420. doi: 10.1016/0304-4157(79)90012-1. [DOI] [PubMed] [Google Scholar]
  8. Haydon D. A., Hladky S. B. Ion transport across thin lipid membranes: a critical discussion of mechanisms in selected systems. Q Rev Biophys. 1972 May;5(2):187–282. doi: 10.1017/s0033583500000883. [DOI] [PubMed] [Google Scholar]
  9. Kemp G., Wenner C. Solution, interfacial, and membrane properties of gramicidin A. Arch Biochem Biophys. 1976 Oct;176(2):547–555. doi: 10.1016/0003-9861(76)90198-3. [DOI] [PubMed] [Google Scholar]
  10. Killian J. A., Timmermans J. W., Keur S., de Kruijff B. The tryptophans of gramicidin are essential for the lipid structure modulating effect of the peptide. Biochim Biophys Acta. 1985 Oct 24;820(1):154–156. doi: 10.1016/0005-2736(85)90227-5. [DOI] [PubMed] [Google Scholar]
  11. Macdonald P. M., Seelig J. Dynamic properties of gramicidin A in phospholipid membranes. Biochemistry. 1988 Apr 5;27(7):2357–2364. doi: 10.1021/bi00407a017. [DOI] [PubMed] [Google Scholar]
  12. Masotti L., Cavatorta P., Sartor G., Casali E., Szabo A. G. Tryptophan interactions of gramicidin A' channels in lipids: a time-resolved fluorescence study. Biochim Biophys Acta. 1986 Nov 17;862(2):265–272. doi: 10.1016/0005-2736(86)90227-0. [DOI] [PubMed] [Google Scholar]
  13. Myers V. B., Haydon D. A. Ion transfer across lipid membranes in the presence of gramicidin A. II. The ion selectivity. Biochim Biophys Acta. 1972 Aug 9;274(2):313–322. doi: 10.1016/0005-2736(72)90179-4. [DOI] [PubMed] [Google Scholar]
  14. Nozaki Y., Tanford C. The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. Establishment of a hydrophobicity scale. J Biol Chem. 1971 Apr 10;246(7):2211–2217. [PubMed] [Google Scholar]
  15. SARGES R., WITKOP B. GRAMICIDIN. VII. THE STRUCTURE OF VALINE- AND ISOLEUCINE-GRAMICIDIN B. J Am Chem Soc. 1965 May 5;87:2027–2030. doi: 10.1021/ja01087a029. [DOI] [PubMed] [Google Scholar]
  16. Spisni A., Khaled M. A., Urry D. W. Temperature-induced incorporation of gramicidin A into lysolecithin micelles demonstrated by 13C NMR. FEBS Lett. 1979 Jun 15;102(2):321–324. doi: 10.1016/0014-5793(79)80027-7. [DOI] [PubMed] [Google Scholar]
  17. Szabo G., Urry D. W. N-acetyl gramicidin: single-channel properties and implications for channel structure. Science. 1979 Jan 5;203(4375):55–57. doi: 10.1126/science.83000. [DOI] [PubMed] [Google Scholar]
  18. Urry D. W., Long M. M., Jacobs M., Harris R. D. Conformation and molecular mechanisms of carriers and channels. Ann N Y Acad Sci. 1975 Dec 30;264:203–220. doi: 10.1111/j.1749-6632.1975.tb31484.x. [DOI] [PubMed] [Google Scholar]
  19. Urry D. W. The gramicidin A transmembrane channel: a proposed pi(L,D) helix. Proc Natl Acad Sci U S A. 1971 Mar;68(3):672–676. doi: 10.1073/pnas.68.3.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weinstein S., Wallace B. A., Blout E. R., Morrow J. S., Veatch W. Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4230–4234. doi: 10.1073/pnas.76.9.4230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weinstein S., Wallace B. A., Morrow J. S., Veatch W. R. Conformation of the gramicidin A transmembrane channel: A 13C nuclear magnetic resonance study of 13C-enriched gramicidin in phosphatidylcholine vesicles. J Mol Biol. 1980 Oct 15;143(1):1–19. doi: 10.1016/0022-2836(80)90121-7. [DOI] [PubMed] [Google Scholar]

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