Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1979 Aug;293:447–456. doi: 10.1113/jphysiol.1979.sp012899

Cold acclimation in the secretory responses of the isolated exocrine pancreas of the rat

Y Habara 1, T Kanno 1, A Saito 1
PMCID: PMC1280723  PMID: 501617

Abstract

1. The secretory function of the isolated and perfused exocrine pancreas has been studied in the course of cold acclimation in rats that were fed at an ambient temperature of 1 °C in an artificial climatic room.

2. A series of experiments was carried out in early autumn (autumn series). The sums of the outputs of protein, amylase, zymogen protease, lipase, and juice flow in samples collected for a total of 30 min during and after 5 min stimulation with different concentrations of cholecystokinin—pancreozymin (CCK—PZ; 5-50 m-u./ml.) were simultaneously measured in the autumn series of experiments, and they were plotted against the logarithmic scale of concentration of CCK—PZ to obtain a dose—response relation.

3. The sums of protein output, lipase output, and juice flow evoked by different concentrations of CCK—PZ in the group of rats fed at 1 °C for 3 weeks were almost identical to the corresponding value in the group fed at about 21 °C for 3 weeks.

4. The sum of output of zymogen protease in the former group was almost identical to the corresponding value in the latter group except that the sum induced by 20 m-u. CCK—PZ/ml. was significantly larger in the former.

5. The sum of output of amylase induced by various concentrations of CCK—PZ in the former group was significantly smaller than the corresponding value in the latter group.

6. It was thus concluded that the CCK—PZ-induced outputs of protein, lipase, and zymogen protease were little affected if any by 3 weeks of cold exposure, whereas the CCK—PZ-induced amylase output was significantly suppressed.

7. Another similar series of experiments was performed in the summer of the same year. The results obtained suggest that the secretory function of exocrine pancreas is directly inhibited by prolonged cold exposure in summer but it is not in autumn.

Full text

PDF
447

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrowman J. A., Mayston P. D. Proceedings: The trophic influence of cholecystokinin on the rat pancreas. J Physiol. 1974 Apr;238(1):73P–75P. [PubMed] [Google Scholar]
  2. Beck L. V., Zaharko D. S., Kalser S. C. Variation in serum insulin and glucose of rats with chronic cold exposure. Life Sci. 1967 Jul 15;6(14):1501–1506. doi: 10.1016/0024-3205(67)90330-x. [DOI] [PubMed] [Google Scholar]
  3. COTTLE W., CARLSON L. D. Adaptive changes in rats exposed to cold; caloric exchange. Am J Physiol. 1954 Aug;178(2):305–308. doi: 10.1152/ajplegacy.1954.178.2.305. [DOI] [PubMed] [Google Scholar]
  4. DEPOCAS F., HART J. S., HEROUX O. Cold acclimation and the electromyogram of unanesthetized rats. J Appl Physiol. 1956 Nov;9(3):404–408. doi: 10.1152/jappl.1956.9.3.404. [DOI] [PubMed] [Google Scholar]
  5. Dagorn J. C., Mongeau R. Different action of hormonal stimulation on the biosynthesis of three pancreatic enzymes. Biochim Biophys Acta. 1977 Jun 23;498(1):76–82. doi: 10.1016/0304-4165(77)90088-5. [DOI] [PubMed] [Google Scholar]
  6. Deschodt-Lanckman M., Robberecht P., Camus J., Christophe J. Short-term adaptation of pancreatic hydrolases to nutritional and physiological stimuli in adult rats. Biochimie. 1971;53(6):789–796. doi: 10.1016/s0300-9084(71)80120-7. [DOI] [PubMed] [Google Scholar]
  7. Harada E., Kanno T. Progressive enhancement in the secretory functions of the digestive system of the rat in the course of cold acclimation. J Physiol. 1976 Sep;260(3):629–645. doi: 10.1113/jphysiol.1976.sp011536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. IVY A. C., JANECEK H. M. Assay of Jorpes-Mutt secretin and cholecystokinin. Acta Physiol Scand. 1959 Mar 31;45:220–230. doi: 10.1111/j.1748-1716.1959.tb01693.x. [DOI] [PubMed] [Google Scholar]
  9. Janský L. Non-shivering thermogenesis and its thermoregulatory significance. Biol Rev Camb Philos Soc. 1973 Feb;48(1):85–132. doi: 10.1111/j.1469-185x.1973.tb01115.x. [DOI] [PubMed] [Google Scholar]
  10. Kanno T., Saito A., Sato Y. Stimulus-secretion coupling in pancreatic acinar cells: influences of external sodium and calcium on responses to cholecystokinin-pancreozymin and ionophore A23187. J Physiol. 1977 Aug;270(1):9–28. doi: 10.1113/jphysiol.1977.sp011935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kanno T., Suga T., Yamamoto M. Effects of oxygen supply on electrical and secretory responses of humorally stimulated acinar cells in isolated rat pancreas. Jpn J Physiol. 1976;26(2):101–115. doi: 10.2170/jjphysiol.26.101. [DOI] [PubMed] [Google Scholar]
  12. Kanno T. The electrogenic sodium pump in the hyperpolarizing and secretory effects of pancreozymin in the pancreatic acinar cell. J Physiol. 1975 Mar;245(3):599–616. doi: 10.1113/jphysiol.1975.sp010864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lagerspetz K. Y. Temperature acclimation and the nervous system. Biol Rev Camb Philos Soc. 1974 Nov;49(4):477–514. doi: 10.1111/j.1469-185x.1974.tb01172.x. [DOI] [PubMed] [Google Scholar]
  15. Lippi U., Stevanato G., Guidi G. A rapid photometric micromethod for serum lipase determination. Clin Chim Acta. 1972 Mar;37:199–202. doi: 10.1016/0009-8981(72)90433-0. [DOI] [PubMed] [Google Scholar]
  16. Malaisse-Lagae F., Ravazzola M., Robberecht P., Vandermeers A., Malaisse W. J., Orci L. Exocrine pancreas: evidence for topographic partition of secretory function. Science. 1975 Nov 21;190(4216):795–797. doi: 10.1126/science.1105788. [DOI] [PubMed] [Google Scholar]
  17. Porte D., Jr, Williams R. H. Inhibition of insulin release by norepinephrine in man. Science. 1966 May 27;152(3726):1248–1250. doi: 10.1126/science.152.3726.1248. [DOI] [PubMed] [Google Scholar]
  18. Rothman S. S., Wells H. Enhancement of pancreatic enzyme synthesis by pancreozymin. Am J Physiol. 1967 Jul;213(1):215–218. doi: 10.1152/ajplegacy.1967.213.1.215. [DOI] [PubMed] [Google Scholar]
  19. Saito A., Kanno T. Concentration of pancreozymin as a determinant of the exocrine-endocrine partition of pancreatic enzymes. Jpn J Physiol. 1973 Oct;23(5):477–495. doi: 10.2170/jjphysiol.23.477. [DOI] [PubMed] [Google Scholar]
  20. Scratcherd T., Case R. M. Perfusion of the pancreas. Gut. 1973 Jul;14(7):592–598. doi: 10.1136/gut.14.7.592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sewell W. A., Young J. A. Secretion of electrolytes by the pancreas of the anaestetized rat. J Physiol. 1975 Nov;252(2):379–396. doi: 10.1113/jphysiol.1975.sp011149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Snook J. T. Effect of diet, adrenalectomy, diabetes, and actinomycin D on exocrine pancreas. Am J Physiol. 1968 Dec;215(6):1329–1333. doi: 10.1152/ajplegacy.1968.215.6.1329. [DOI] [PubMed] [Google Scholar]
  23. Söling H. D., Unger K. O. The role of insulin in the regulation of -amylase synthesis in the rat pancreas. Eur J Clin Invest. 1972 Jun;2(4):199–212. doi: 10.1111/j.1365-2362.1972.tb00645.x. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES