Abstract
The relaxation behavior of intracellular 23Na in suspensions of chicken erythrocytes and of their nuclei was investigated. The transverse magnetization was found to decay biexponentially. The average relaxation rates for the nucleated chicken erythrocytes are considerably shorter than the average relaxation rates obtained for dog and human nonnucleated red blood cells. Of particular significance is the twofold decrease in the short component of T2. Calculations based on the measured 23Na NMR relaxation rates in suspensions of nuclei indicate that most of the difference between the relaxation rates in the mammalian as compared to the chicken erythrocytes, can be accounted for by the contribution of the nuclei in the latter.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aaronson R. P., Blobel G. On the attachment of the nuclear pore complex. J Cell Biol. 1974 Sep;62(3):746–754. doi: 10.1083/jcb.62.3.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gupta R. K., Gupta P., Moore R. D. NMR studies of intracellular metal ions in intact cells and tissues. Annu Rev Biophys Bioeng. 1984;13:221–246. doi: 10.1146/annurev.bb.13.060184.001253. [DOI] [PubMed] [Google Scholar]
- Nordenskiöld L., Chang D. K., Anderson C. F., Record M. T., Jr 23Na NMR relaxation study of the effects of conformation and base composition on the interactions of counterions with double-helical DNA. Biochemistry. 1984 Sep 11;23(19):4309–4317. doi: 10.1021/bi00314a009. [DOI] [PubMed] [Google Scholar]
- Ogino T., Shulman G. I., Avison M. J., Gullans S. R., den Hollander J. A., Shulman R. G. 23Na and 39K NMR studies of ion transport in human erythrocytes. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1099–1103. doi: 10.1073/pnas.82.4.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shinar H., Navon G. NMR relaxation studies of intracellular Na+ in red blood cells. Biophys Chem. 1984 Nov;20(4):275–283. doi: 10.1016/0301-4622(84)80018-6. [DOI] [PubMed] [Google Scholar]
- Shinar H., Navon G. The determination of intracellular water space by NMR. FEBS Lett. 1985 Nov 25;193(1):75–78. doi: 10.1016/0014-5793(85)80082-x. [DOI] [PubMed] [Google Scholar]
- Springer C. S., Jr Measurement of metal cation compartmentalization in tissue by high-resolution metal cation NMR. Annu Rev Biophys Biophys Chem. 1987;16:375–399. doi: 10.1146/annurev.bb.16.060187.002111. [DOI] [PubMed] [Google Scholar]
- Szuchet S., Polak P. E. A simple cell disrupter designed for small cells with relatively large nuclei. Anal Biochem. 1983 Feb 1;128(2):453–458. doi: 10.1016/0003-2697(83)90400-1. [DOI] [PubMed] [Google Scholar]