Abstract
A procedure is described which optimizes nonnegative least squares and exponential sampling fitting methods for analysis of dynamic light scattering (DLS) data from aqueous suspensions of vesicle/liposome systems. This approach utilizes a Rayleigh-Gans-Debye form factor for a coated sphere and yields number distributions which can be compared directly to distributions obtained by freeze-fracture electron microscopy (EM). Excellent agreement between the DLS and EM results are obtained for vesicle size distributions in the 100-200-nm range.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Day E. P., Ho J. T., Kunze R. K., Jr, Sun S. T. Dynamic light scattering study of calcium-induced fusion in phospholipid vesicles. Biochim Biophys Acta. 1977 Nov 1;470(3):503–508. doi: 10.1016/0005-2736(77)90142-0. [DOI] [PubMed] [Google Scholar]
- Fujime S., Takasaki-Ohsita M., Miyamoto S. Dynamic light scattering from polydisperse suspensions of thin ellipsoidal shells of revolution with submicron diameters. Biophys J. 1988 Apr;53(4):497–503. doi: 10.1016/S0006-3495(88)83129-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goll J. H., Stock G. B. Determination by photon correlation spectroscopy of particle size distributions in lipid vesicle suspensions. Biophys J. 1977 Sep;19(3):265–273. doi: 10.1016/S0006-3495(77)85586-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goll J., Carlson F. D., Barenholz Y., Litman B. J., Thompson T. E. Photon correlation spectroscopic study of the size distribution of phospholipid vesicles. Biophys J. 1982 Apr;38(1):7–13. doi: 10.1016/S0006-3495(82)84524-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haines T. H., Li W., Green M., Cummins H. Z. The elasticity of uniform, unilamellar vesicles of acidic phospholipids during osmotic swelling is dominated by the ionic strength of the media. Biochemistry. 1987 Aug 25;26(17):5439–5447. doi: 10.1021/bi00391a034. [DOI] [PubMed] [Google Scholar]
- Hantz E., Cao A., Escaig J., Taillandier E. The osmotic response of large unilamellar vesicles studied by quasielastic light scattering. Biochim Biophys Acta. 1986 Nov 17;862(2):379–386. doi: 10.1016/0005-2736(86)90241-5. [DOI] [PubMed] [Google Scholar]
- Li W., Aurora T. S., Haines T. H., Cummins H. Z. Elasticity of synthetic phospholipid vesicles and submitochondrial particles during osmotic swelling. Biochemistry. 1986 Dec 16;25(25):8220–8229. doi: 10.1021/bi00373a015. [DOI] [PubMed] [Google Scholar]
- Li W., Haines T. H. Uniform preparations of large unilamellar vesicles containing anionic lipids. Biochemistry. 1986 Nov 18;25(23):7477–7483. doi: 10.1021/bi00371a033. [DOI] [PubMed] [Google Scholar]
- Mayer L. D., Hope M. J., Cullis P. R. Vesicles of variable sizes produced by a rapid extrusion procedure. Biochim Biophys Acta. 1986 Jun 13;858(1):161–168. doi: 10.1016/0005-2736(86)90302-0. [DOI] [PubMed] [Google Scholar]
- McCracken M. S., Sammons M. C. Sizing of a vesicle drug formulation by quasi-elastic light scattering and comparison with electron microscopy and ultracentrifugation. J Pharm Sci. 1987 Jan;76(1):56–59. doi: 10.1002/jps.2600760116. [DOI] [PubMed] [Google Scholar]
- Miyamoto S., Maeda T., Fujime S. Change in membrane elastic modulus on activation of glucose transport system of brush border membrane vesicles studied by osmotic swelling and dynamic light scattering. Biophys J. 1988 Apr;53(4):505–512. doi: 10.1016/S0006-3495(88)83130-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Olson F., Hunt C. A., Szoka F. C., Vail W. J., Papahadjopoulos D. Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes. Biochim Biophys Acta. 1979 Oct 19;557(1):9–23. doi: 10.1016/0005-2736(79)90085-3. [DOI] [PubMed] [Google Scholar]
- Perevucnik G., Schurtenberger P., Lasic D. D., Hauser H. Size analysis of biological membrane vesicles by gel filtration, dynamic light scattering and electron microscopy. Biochim Biophys Acta. 1985 Nov 21;821(1):169–173. doi: 10.1016/0005-2736(85)90168-3. [DOI] [PubMed] [Google Scholar]
- Ruf H., Georgalis Y., Grell E. Dynamic laser light scattering to determine size distributions of vesicles. Methods Enzymol. 1989;172:364–390. doi: 10.1016/s0076-6879(89)72024-3. [DOI] [PubMed] [Google Scholar]
- Sattelle D. B., Langley K. H., Obaid A. L., Salzberg B. M. Laser light scattering determination of size and dispersity of synaptosomes and synaptic vesicles isolated from squid (Loligo pealei) optic lobes. Eur Biophys J. 1987;15(2):71–76. doi: 10.1007/BF00257500. [DOI] [PubMed] [Google Scholar]
- Selser J. C., Yeh Y., Baskin R. J. A light-scattering characterization of membrane vesicles. Biophys J. 1976 Apr;16(4):337–356. doi: 10.1016/S0006-3495(76)85692-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selser J. C., Yeh Y., Baskin R. J. A light-scattering measurement of membrane vesicle permeability. Biophys J. 1976 Dec;16(12):1357–1371. doi: 10.1016/S0006-3495(76)85780-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selser J. C., Yeh Y. Letter: A light scattering method of measuring membrane vesicle number-averaged size and size dispersion. Biophys J. 1976 Jul;16(7):847–848. doi: 10.1016/S0006-3495(76)85734-7. [DOI] [PMC free article] [PubMed] [Google Scholar]