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. 1991 Apr;59(4):880–888. doi: 10.1016/S0006-3495(91)82300-1

Cross-helix separation of tropomyosin molecules in acto-tropomyosin as determined by neutron scattering.

D B Bivin 1, D B Stone 1, D K Schneider 1, R A Mendelson 1
PMCID: PMC1281253  PMID: 1829644

Abstract

The cross-helix separation of Tm molecules in acto-tropomyosin has been determined using neutron scattering. Deuterated Dictyostelium discoideum actin was density matched in a 93% D2O buffer so that effectively only the protonated tropomyosin was "visible" to neutrons. Analysis of the solution scattering pattern in the region of the first oscillation yielded a value for the cross-helix separation of 7.9 +/- 0.3 nm. The implications of this value for the mechanism of the regulation of muscle contraction are discussed in light of recent results by others.

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Selected References

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