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. 1979 Mar;288:211–225.

Reciprocal transplantations between the optic tectum and the cerebellum in adult goldfish.

M G Yoon
PMCID: PMC1281423  PMID: 469717

Abstract

1. The topographic pattern of re-established visual projections after a reciprocal transplantation between tectal and cerebellar tissues was studied in adult goldfish. 2. A rectangular tissue was dissected from the left tectum, and a similar piece from the cerebellum in the same fish. The cerebellar piece was rotated by either 180 or 0 degrees around the dorsoventral axis, and transplanted into the tectum in place of the tectal piece. This tectal tissue was likewise grafted into the cerebellum after either 180 or 0 degrees rotation in the same fish. 3. The tectal graft disappeared from the cerebellum within 2 months after surgery. The operated cerebellum showed a remarkable capability for healing its excised part. No visual responses were recorded from the cerebellum. 4. The cerebellar grafts remained in place within the operated tectum in twenty fish. In fifteen of these fish, tested 3 or 4 months after surgery, the cerebellar grafts did not give any visual responses, unlike the surrounding responsive area of the tectum. These fish showed a partial scotoma in the central area of the visual field, which corresponded to the unresponsive transplanted area of the tectum. Autoradiographic examination after intraocular injection of L-[3H]proline showed that these cerebellar grafts did not contain any noticeable label, in contrast to the extensively labelled surrounding tectal tissues. 5. Sporadic visual responses were recorded from deep layers in the transplanted area of the tectum in five of the twenty fish at early post-operative periods. The receptive fields of these responses were distributed in a correct retinotopic order, regardless of whether the cerebellar tissue had been rotated by either 180 or 0 degrees. Autoradiographic examination, however, revealed that these cerebellar grafts were not invaded by regenerating optic fibres. Instead, they bypassed the interposed cerebellar tissue by making detours beneath the graft on the way towards their appropriate target zones within the tectal tissue. 6. This selective avoidance of a foreign (cerebellar) tissue and the orderly reinnervation of the proper tectal tissue by regenerating optic fibres provide us with further evidence for neuronal specificity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chung S. H., Cooke J. Polarity of structure and of ordered nerve connections in the developing amphibian brain. Nature. 1975 Nov 13;258(5531):126–132. doi: 10.1038/258126a0. [DOI] [PubMed] [Google Scholar]
  2. Gaze R. M., Sharma S. C. Axial differences in the reinnervation of the goldfish optic tectum by regenerating optic nerve fibres. Exp Brain Res. 1970;10(2):171–181. [PubMed] [Google Scholar]
  3. Jacobson M., Levine R. L. Stability of implanted duplicate tectal positional markers serving as targets for optic axons in adult frogs. Brain Res. 1975 Jul 18;92(3):468–471. doi: 10.1016/0006-8993(75)90332-7. [DOI] [PubMed] [Google Scholar]
  4. Levine R., Jacobson M. Deployment of optic nerve fibers is determined by positional markers in the frog's tectum. Exp Neurol. 1974 Jun;43(3):527–538. doi: 10.1016/0014-4886(74)90192-7. [DOI] [PubMed] [Google Scholar]
  5. Sharma S. C., Gaze R. M. The retinotopic organization of visual responses from tectal reimplants in adult goldfish. Arch Ital Biol. 1971 Dec;109(4):357–366. [PubMed] [Google Scholar]
  6. Sharma S. C. Redistribution of visual projections in altered optic tecta of adult goldfish. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2637–2639. doi: 10.1073/pnas.69.9.2637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Yoon M. G. Induction of compression in the re-established visual projections on to a rotated tectal reimplant that retains its original topographic polarity within the halved optic tectum of adult goldfish. J Physiol. 1977 Jan;264(2):379–410. doi: 10.1113/jphysiol.1977.sp011674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yoon M. G. Readjustment of retinotectal projection following reimplantation of a rotated or inverted tectal tissue in adult goldfish. J Physiol. 1975 Oct;252(1):137–158. doi: 10.1113/jphysiol.1975.sp011138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yoon M. G. Retention of the original topographic polarity by the 180 degrees rotated tectal reimplant in young adult goldfish. J Physiol. 1973 Sep;233(3):575–588. doi: 10.1113/jphysiol.1973.sp010324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Yoon M. G. Topographic polarity of the optic tectum studied by reimplantation of the tectal tissue in adult goldfish. Cold Spring Harb Symp Quant Biol. 1976;40:503–519. doi: 10.1101/sqb.1976.040.01.047. [DOI] [PubMed] [Google Scholar]
  11. Yoon M. G. Transposition of the visual projection from the nasal hemiretina onto the foreign rostral zone of the optic tectum in goldfish. Exp Neurol. 1972 Dec;37(3):451–462. doi: 10.1016/0014-4886(72)90088-x. [DOI] [PubMed] [Google Scholar]
  12. Yoon M. Reorganization of retinotectal projection following surgical operations on the optic tectum in goldfish. Exp Neurol. 1971 Nov;33(2):395–411. doi: 10.1016/0014-4886(71)90031-8. [DOI] [PubMed] [Google Scholar]

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