In the published article, Figure 4F was retouched to eliminate three artifacts from toluidine blue staining. These artifactual blue deposits were retouched for aesthetic purposes, and the retouching does not impact the interpretation of the data nor the conclusions of the study.
Figure 4.
(A–F) Photomicrographs of testicular sections of animals from CG and IG stained with H.E. (A-D), and semithin sections stained with toluidine blue (E, F). In (A), normal histoarchitecture of the seminiferous tubules (ST) and interstitial tissue (IT) is observed in CG. However, in (B) (IG), intraepithelial spaces are seen in the seminiferous tubules (ST; stars). IT, interstitial tissue. In (C), the interstitial tissue exhibits typical LCs with round/ovoid nucleus (thick arrows) whereas in (D), these cells show irregular and reduced nucleus (thick arrows), and numerous lipid inclusions (asterisks) in comparison to CG. In (E, F), semithin sections show interstitial tissue containing macrophages (thin arrows) and LCs (thick arrows). Numerous and large lipid inclusions (asterisks) are observed in the LCs of IG when compared to CG. Note the spirally arranged cisternae in these cells (insets; arrowheads). (G) Significant reduction of LC nuclear area is observed in IG in comparison to CG. (H–J) A significant increase in the mRNA expression of Srebp1, Dgat-1 and Scarb1 is observed in IG when compared to CG. *p value.
The original figure is illustrated below.
In the published article, there were errors in Table 1.
Table 1.
Primary and secondary antibodies.
| Antibody name | Dilution | Catalog number | Manufacturer | RRID |
|---|---|---|---|---|
| Mouse anti-ACE2 monoclonal antibody | 1:50 | sc-73668 | Santa Cruz Biotechnology, USA | AB_2861379 |
| Rabbit anti-SARS-CoV-2 Spike Protein S1 Recombinant monoclonal antibody | 1:250 | MA5-36247 | Invitrogen by Thermo Fisher Scientific | AB_2890589 |
| Rabbit anti-SARS-CoV-2 nucleocapsid protein monoclonal antibody | 1:3000 | ab271180 | Abcam, Cambridge, UK | – |
| Rabbit anti-IL-1β polyclonal antibody | 1:400 | ab9722 | Abcam, Cambridge, UK | AB_308765 |
| Goat anti-IL-6 (M19) polyclonal antibody | 1:400 | sc-1265 | Santa Cruz Biotechnology, USA | AB_2127470 |
| Mouse anti-TNF-α monoclonal [52B83] antibody | 1:200 | ab1793 | Abcam, Cambridge, UK | AB_302615 |
| Rabbit anti-CD163 monoclonal antibody | 1:100 | ab182422 | Abcam, Cambridge, UK | AB_2753196 |
| Rabbit anti-CD68 polyclonal antibody | 1:100 | ab125212 | Abcam, Cambridge, UK | AB_10975465 |
| Rabbit anti-17β-HSD6 polyclonal antibody | 1:500 | sc-393936 | Santa Cruz Biotechnology, USA | AB_2891064 |
| Rabbit anti-StAR polyclonal antibody | 1:1000 | PAS-95765 | Invitrogen by Thermo Fisher Scientific | – |
| Mouse anti-testosterone polyclonal antibody | 1:100 | ab217912 | Abcam, Cambridge, UK | AB_308765 |
| Rabbit anti-IL-10 polyclonal antibody | 1:500 | sc-8438 | Santa Cruz Biotechnology, USA | AB_627793 |
| Rabbit anti-MIF polyclonal antibody | 1:200 | 251415 | ABBIOTEC, USA | AB_10636927 |
| Rabbit anti-β-tubulin monoclonal antibody | 1:8000 | ab108342 | Abcam, Cambridge, UK | AB_10866289 |
| HRP-conjugated anti-rabbit secondary antibody | 1:9000 | A9169 | Sigma-Aldrich, USA | |
| Alexa Fluor®488 anti-mouse antibody | 1:1000 | ab150113 | Abcam, Cambridge, UK | AB_2576208 |
| Alexa Fluor®488 anti-rabbit antibody | 1:1000 | ab150077 | Abcam, Cambridge, UK | AB_2630356 |
| Alexa Fluor®594 anti-mouse antibody | 1:1000 | ab150116 | Abcam, Cambridge, UK | AB_2650601 |
| Alexa Fluor® 647 anti-rabbit antibody | 1:1000 | ab150075 | Abcam, Cambridge, UK | AB_2752244 |
| Alexa Fluor®647 anti-goat antibody | 1:1000 | ab150135 | Abcam, Cambridge, UK | AB_2687955 |
The antibody “Donkey anti IL-6 (M19) polyclonal antibody” is incorrect. The actual antibody used was “Goat anti-IL-6 (M19) polyclonal antibody”. The “mouse anti-17beta-HSD7 antibody; 1:500 sc-393936; Santa Cruz Biotechnology, USA AB_2891064” is incorrect. The actual antibody used was the “Rabbit anti-17beta-HSD6; 2μg/ml; sc-101878; Santa Cruz Biotechnology, USA”. Regarding Alexa Fluor® 488 anti-mouse antibody, the “catalog number (A11001), manufacturer (Molecular Probes by Life Technologies) and RRID” are incorrect. The correct is “catalog number ab150113; manufacturer (Abcam, Cambridge, UK) and RRID (AB_2576208)”. The data regarding the “Alexa Fluor® 594 anti-rabbit antibody” are incorrect. The correct is “Alexa Fluor® 647 anti-rabbit antibody; dilution 1:1000, catalog number (ab150075); manufacturer (Abcam, Cambridge, UK); RRID (AB_2752244)”. The “Alexa Fluor® 647 anti-donkey polyclonal antibody” is incorrect. The correct is “Alexa Fluor® 647 anti-goat antibody; dilution 1:1000; RRID (AB_2687955)”. The “Alexa Fluor® 488 anti-rabbit antibody, Alexa Fluor® 594 anti-mouse antibody, and their respective dilution, catalog number, manufacturer and RRID” were missing and were added to Table 1. The corrected Table 1 is below.
Methods (items 2.6 - page 4 and 2.7 -page 5), where it reads “17beta-HSD7”, the correct word is “17beta-HSD6”; this was incorrectly described by mistake.
A correction has been made to the sections:
2. Material and methods, 2. 6 Immunohistochemistry and immunofluorescence analyses, Paragraph 2, Line 9: “anti-17β-HSD6 polyclonal antibody”
2.7 Double immunofluorescence analysis, Lines 4-11:
“Moreover, to confirm if the LCs express an inflammatory profile, double immunofluorescences for detection of 17β-HSD6+IL-6, 17β-HSD6+IL-1β and StAR+TNF-α were also performed. The double immunofluorescence reactions were performed according to de Santi et al. (2022). After antigen recovery, the sections were incubated overnight at 4°C with the following primary antibodies (Table 1): anti-human ACE2 monoclonal antibody, anti-17β-HSD6 polyclonal antibody or anti-StAR polyclonal antibody.”
Legend: Figure 1 (J, K): where it reads “17beta-HSD7”, the correct word is “17beta-HSD6”.
The original version of this article has been updated.
Footnotes
Edited and reviewed by: Katarzyna Otulak-Kozieł, Warsaw University of Life Sciences, Poland
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