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. 1978 Dec;285:395–408. doi: 10.1113/jphysiol.1978.sp012578

The metabolic and endocrine effects of circulating catecholamines in fetal sheep.

C T Jones, J W Ritchie
PMCID: PMC1281763  PMID: 217988

Abstract

1. Adrenaline and noradrenaline have been infused into the fetal sheep to produce plasma concentrations comparable to those seen during hypoxia. The effects have been compared with those of isoprenaline and methoxamine and the sensitivity to beta- and alpha-adrenergic antagonists has been followed. 2. Adrenaline caused an alpha-mediated increase in blood glucose that is associated with a fall in plasma insulin concentration. It also caused a beta-mediated increase in plasma lactate, free fatty acid and amino acid concentrations. 3. Noradrenaline was much less effective than adrenaline at eliciting metabolic responses. It had not cause a significant change in plasma glucose concentration, although this was associated with a small increase in plasma insulin concentration. It caused a small rise in the concentration of lactate and free fatty acids in fetal plasma but had no effect on plasma amino acids. 4. The beta- and alpha-adrenergic antagonists propranolol and phentolamine alone were without effect on any of the plasma metabolites or hormones assayed. Isoprenaline increased plasma glucose, lactate, free fatty acid, alanine and insulin concentrations, while methoxamine only increased plasma glucose and lactate, and this was associated with a fall in insulin concentration. 5. The concentration of ACTH in fetal plasma was increased by adrenaline and to a lesser extent by noradrenaline and methoxamine; these were blocked by phentolamine. Isoprenaline also caused a small increase in ACTH. There were no corticosteroid changes associated with the increase in ACTH. 6. The results have been discussed in relation to the adrenergic and pancreatic control of metabolism in the fetal sheep.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALLARD F. J., OLIVER I. T. CARBOHYDRATE METABOLISM IN LIVER FROM FOETAL AND NEONATAL SHEEP. Biochem J. 1965 Apr;95:191–200. doi: 10.1042/bj0950191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BODY D. R., SHORLAND F. B. MATERNAL AND FOETAL LIPIDS OF SHEEP. Nature. 1964 May 23;202:769–769. doi: 10.1038/202769a0. [DOI] [PubMed] [Google Scholar]
  3. Barrett C. T., Heymann M. A., Rudolph A. M. Alpha and beta adrenergic receptor activity in fetal sheep. Am J Obstet Gynecol. 1972 Apr 15;112(8):1114–1121. doi: 10.1016/0002-9378(72)90189-5. [DOI] [PubMed] [Google Scholar]
  4. Bassett J. M. Metabolic effects of catecholamines in sheep. Aust J Biol Sci. 1970 Aug;23(4):903–914. doi: 10.1071/bi9700903. [DOI] [PubMed] [Google Scholar]
  5. Bassett J. M. Plasma glucagon concentrations in sheep: their regulation and relation to concentrations of insulin and growth hormone. Aust J Biol Sci. 1972 Dec;25(6):1277–1287. doi: 10.1071/bi9721277. [DOI] [PubMed] [Google Scholar]
  6. Bassett J. M., Thorburn G. D. The regulation of insulin secretion by the ovine foetus in utero. J Endocrinol. 1971 May;50(1):59–74. doi: 10.1677/joe.0.0500059. [DOI] [PubMed] [Google Scholar]
  7. Birnbaum M. J., Schultz J., Fain J. N. Hormone-stimulated glycogenolysis in isolated goldfish hepatocytes. Am J Physiol. 1976 Jul;231(1):191–197. doi: 10.1152/ajplegacy.1976.231.1.191. [DOI] [PubMed] [Google Scholar]
  8. Bloom S. R., Edwards A. V., Hardy R. N., Silver M. Adrenal and pancreatic endocrine responses to hypoxia in the conscious calf. J Physiol. 1976 Oct;261(2):271–283. doi: 10.1113/jphysiol.1976.sp011558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. COMLINE R. S., SILVER I. A., SILVER M. FACTORS RESPONSIBLE FOR THE STIMULATION OF THE ADRENAL MEDULLA DURING ASPHYXIA IN THE FOETAL LAMB. J Physiol. 1965 May;178:211–238. doi: 10.1113/jphysiol.1965.sp007624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. COMLINE R. S., SILVER M. The release of adrenaline and noradrenaline from the adrenal glands of the foetal sheep. J Physiol. 1961 May;156:424–444. doi: 10.1113/jphysiol.1961.sp006685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cannon B., Romert L., Sundin U., Barnard T. Morphology and biochemical properties of perirenal adipose tissue from lamb (Ovis aries). A comparison with brown adipose tissue. Comp Biochem Physiol B. 1977;56(1):87–99. doi: 10.1016/0305-0491(77)90227-9. [DOI] [PubMed] [Google Scholar]
  12. Comline R. S., Silver M. The composition of foetal and maternal blood during parturition in the ewe. J Physiol. 1972 Apr;222(1):233–256. doi: 10.1113/jphysiol.1972.sp009795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ellis S., Kennedy B. L., Eusebi A. J., Vincent N. H. Autonomic control of metabolism. Ann N Y Acad Sci. 1967 Feb 10;139(3):826–832. doi: 10.1111/j.1749-6632.1967.tb41252.x. [DOI] [PubMed] [Google Scholar]
  14. Exton J. H., Harper S. C. Role of cyclic AMP in the actions of catecholamines on hepatic carbohydrate metabolism. Adv Cyclic Nucleotide Res. 1975;5:519–532. [PubMed] [Google Scholar]
  15. Exton J. H., Mallette L. E., Jefferson L. S., Wong E. H., Friedmann N., Miller T. B., Jr, Park C. R. The hormonal control of hepatic gluconeogenesis. Recent Prog Horm Res. 1970;26:411–461. doi: 10.1016/b978-0-12-571126-5.50014-5. [DOI] [PubMed] [Google Scholar]
  16. Exton J. H., Robison G. A., Sutherland E. W., Park C. R. Studies on the role of adenosine 3',5'-monophosphate in the hepatic actions of glucagon and catecholamines. J Biol Chem. 1971 Oct 25;246(20):6166–6177. [PubMed] [Google Scholar]
  17. FLEMING W. W., KENNY A. D. THE EFFECT OF FASTING ON THE HYPERGLYCAEMIC RESPONSES TO CATECHOL AMINES IN RATS. Br J Pharmacol Chemother. 1964 Apr;22:267–274. doi: 10.1111/j.1476-5381.1964.tb02032.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Fain J. N., Reed N., Saperstein R. The isolation and metabolism of brown fat cells. J Biol Chem. 1967 Apr 25;242(8):1887–1894. [PubMed] [Google Scholar]
  19. GARLAND P. B., RANDLE P. J. EFFECTS OF ALLOXAN DIABETES AND ADRENALINE ON CONCENTRATIONS OF FREE FATTY ACIDS IN RAT HEART AND DIAPHRAGM MUSCLES. Nature. 1963 Jul 27;199:381–382. doi: 10.1038/199381a0. [DOI] [PubMed] [Google Scholar]
  20. GRIFFIN A. C., LUCK J. M., KULAKOFF V., MILLS M. Further observations on the endocrine regulation of blood amino acids. J Biol Chem. 1954 Jul;209(1):387–393. [PubMed] [Google Scholar]
  21. Garber A. J., Karl I. E., Kipnis D. M. Alanine and glutamine synthesis and release from skeletal muscle. IV. beta-Adrenergic inhibition of amino acid release. J Biol Chem. 1976 Feb 10;251(3):851–857. [PubMed] [Google Scholar]
  22. Gemmell R. T., Bell A. W., Alexander G. Morphology of adipose cells in lambs at birth and during subsequent transition of brown to white adipose tissue in cold and in warm conditons. Am J Anat. 1972 Feb;133(2):143–164. doi: 10.1002/aja.1001330203. [DOI] [PubMed] [Google Scholar]
  23. Haylett D. G., Jenkinson D. H. The receptors concerned in the actions of catecholamines on glucose release, membrane potential and ion movements in guinea-pig liver. J Physiol. 1972 Sep;225(3):751–772. doi: 10.1113/jphysiol.1972.sp009967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Hems D. A., Rodrigues L. M., Whitton P. D. Glycogen phosphorylase, glucose output and vasoconstriction in the perfused rat liver. Concentration-dependence of actions of adrenaline, vasopressin and angiotensin II. Biochem J. 1976 Nov 15;160(2):367–374. doi: 10.1042/bj1600367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Himms-Hagen J. Sympathetic regulation of metabolism. Pharmacol Rev. 1967 Sep;19(3):367–461. [PubMed] [Google Scholar]
  26. Iversen J. Adrenergic receptors and the secretion of glucagon and insulin from the isolated, perfused canine pancreas. J Clin Invest. 1973 Sep;52(9):2102–2116. doi: 10.1172/JCI107395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Jakob A., Diem S. Metabolic responses of perfused rat livers to alpha- and beta-adrenergic agonists, glucagon and cyclic AMP. Biochim Biophys Acta. 1975 Sep 8;404(1):57–66. doi: 10.1016/0304-4165(75)90147-6. [DOI] [PubMed] [Google Scholar]
  28. Jones C. T., Boddy K., Robinson J. S., Ratcliffe J. G. Developmental changes in the responses of the adrenal glands of foetal sheep to endogenous adrenocorticotrophin, as indicated by hormone responses to hypoxaemia. J Endocrinol. 1977 Mar;72(3):279–292. doi: 10.1677/joe.0.0720279. [DOI] [PubMed] [Google Scholar]
  29. Jones C. T. Lipid metabolism and mobilization in the guinea pig during pregnancy. Biochem J. 1976 May 15;156(2):357–365. doi: 10.1042/bj1560357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Jones C. T., Ritchie J. W. The cardiovascular effects of circulating catecholamines in fetal sheep. J Physiol. 1978 Dec;285:381–393. doi: 10.1113/jphysiol.1978.sp012577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Jones C. T., Robinson R. O., Luther E., Ritchie J. W., Worthington D. Control of adrenocorticotrophin secretion by catecholamines in the pregnant and foetal sheep. J Endocrinol. 1977 Apr;73(1):11–20. doi: 10.1677/joe.0.0730011. [DOI] [PubMed] [Google Scholar]
  32. Jones C. T., Robinson R. O. Plasma catecholamines in foetal and adult sheep. J Physiol. 1975 Jun;248(1):15–33. doi: 10.1113/jphysiol.1975.sp010960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Jones C. T. The development of some metabolic responses to hypoxia in the foetal sheep. J Physiol. 1977 Mar;265(3):743–762. doi: 10.1113/jphysiol.1977.sp011741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. KIPNIS D. M., HELMREICH E., CORI C. F. Studies of tissue permeability. IV. The distribution of glucose between plasma and muscle. J Biol Chem. 1959 Jan;234(1):165–170. [PubMed] [Google Scholar]
  35. Kumon A., Hara T., Takahashi A. Effects of catecholamines on the lipolysis of two kinds of fat cells from adult rabbit. J Lipid Res. 1976 Nov;17(6):559–564. [PubMed] [Google Scholar]
  36. Kusaka M., Ui M. Activation of the Cori cycle by epinephrine. Am J Physiol. 1977 Feb;232(2):E145–E155. doi: 10.1152/ajpendo.1977.232.2.E145. [DOI] [PubMed] [Google Scholar]
  37. Lagercrantz H., Bistoletti P. Catecholamine release in the newborn infant at birth. Pediatr Res. 1977 Aug;11(8):889–893. doi: 10.1203/00006450-197708000-00007. [DOI] [PubMed] [Google Scholar]
  38. Li J. B., Jefferson L. S. Effect of isoproterenol on amino acid levels and protein turnover in skeletal muscle. Am J Physiol. 1977 Feb;232(2):E243–E249. doi: 10.1152/ajpendo.1977.232.2.E243. [DOI] [PubMed] [Google Scholar]
  39. MAYER S., MORAN N. C., FAIN J. The effect of adrenergic blocking agents on some metabolic actions of catecholamines. J Pharmacol Exp Ther. 1961 Oct;134:18–27. [PubMed] [Google Scholar]
  40. Malaisse W., Malaisse-Lagae F., Wright P. H., Ashmore J. Effects of adrenergic and cholinergic agents upon insulin secretion in vitro. Endocrinology. 1967 May;80(5):975–978. doi: 10.1210/endo-80-5-975. [DOI] [PubMed] [Google Scholar]
  41. Nakai Y., Imura H., Yoshimi T., Matsukura S. Adrenergic control mechanism for ACTH secretion in man. Acta Endocrinol (Copenh) 1973 Oct;74(2):263–270. doi: 10.1530/acta.0.0740263. [DOI] [PubMed] [Google Scholar]
  42. Phillips R. W., House W. A., Miller R. A., Mott J. L., Sooby D. L. Fatty acid, epinephrine, and glucagon hyperglycemia in normal and depancreatized sheep. Am J Physiol. 1969 Nov;217(5):1265–1268. doi: 10.1152/ajplegacy.1969.217.5.1265. [DOI] [PubMed] [Google Scholar]
  43. Porte D., Jr A receptor mechanism for the inhibition of insulin release by epinephrine in man. J Clin Invest. 1967 Jan;46(1):86–94. doi: 10.1172/JCI105514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Rosell S., Belfrage E. Adrenergic receptors in adipose tissue and their relation to adrenergic innervation. Nature. 1975 Feb 27;253(5494):738–739. doi: 10.1038/253738a0. [DOI] [PubMed] [Google Scholar]
  45. Ruderman N. B. Muscle amino acid metabolism and gluconeogenesis. Annu Rev Med. 1975;26:245–258. doi: 10.1146/annurev.me.26.020175.001333. [DOI] [PubMed] [Google Scholar]
  46. Saito Y., Itaya K., Ui M. Adrenergic alpha-receptor-mediated stimulation of the glucose utilization by isolated rat diaphragm. Biochim Biophys Acta. 1974 May 24;343(3):492–499. doi: 10.1016/0304-4165(74)90266-9. [DOI] [PubMed] [Google Scholar]
  47. Shimazu T., Amakawa A. Regulation of glycogen metabolism in liver by the autonomic nervous system. VI. Possible mechanism of phosphorylase activation by the splanchnic nerve. Biochim Biophys Acta. 1975 Apr 7;385(2):242–256. doi: 10.1016/0304-4165(75)90352-9. [DOI] [PubMed] [Google Scholar]
  48. Tolbert M. E., Butcher F. R., Fain J. N. Lack of correlation between catecholamine effects on cyclic adenosine 3':5'-monophosphate and gluconeogenesis in isolated rat liver cells. J Biol Chem. 1973 Aug 25;248(16):5686–5692. [PubMed] [Google Scholar]
  49. UI M. ACTION OF EPINEPHRINE ON MUSCLE GLUCOSE UPTAKE DEPENDING ON CA++ AND PHOSPHATE. Am J Physiol. 1965 Aug;209:359–364. doi: 10.1152/ajplegacy.1965.209.2.359. [DOI] [PubMed] [Google Scholar]
  50. Wensvoort P. The development of adipose tissue in sheep foetuses. Pathol Vet. 1967;4(1):69–78. doi: 10.1177/030098586700400107. [DOI] [PubMed] [Google Scholar]

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