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. 2025 Dec 10;22:24. doi: 10.1186/s12917-025-05164-2

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© The Author(s) 2025

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Fig. 2 — Expression and purification of the NSP4-ΔHR protein. A Expression of pET28a-NSP4-ΔHR in E. coli BL21(DE3) was analyzed by SDS-PAGE. Lane M, protein molecular weight marker; Lane 1, ultrasonic lysis of the whole bacterial solution; Lane 2, lysate supernatant; Lane 3, lysate pellet. B SDS-PAGE analysis of purification of NSP4-ΔHR recombinant protein. After ultrasonic centrifugation, post-sonication supernatant was incubated with Ni-NTA resin for 1 h, and elution with 150 mM (lane 1), 200 mM (lane 2), 250 mM (lane 3), and 300 mM (lane 4) imidazole. Lane M: protein molecular weight marker. C Western blot analysis of NSP4-ΔHR by His-tag monoclonal antibody