Fig. 5.

Amino Acid Truncation Expression and Alanine Mutation Analysis of mAb 7H10. A Schematic diagram of the B-cell epitope mapping of NSP4-ΔHR protein recognized by mAb 7H10. Two amino acids from the N-terminus or C-terminus were truncated respectively. The red amino acids indicate peptides that reacted with mAb 7H10, while grey amino acids represent peptides that did not react with mAb7H10. B The peptides expressed were analyzed by SDS-PAGE Coomassie brilliant blue staining and Western blot with mAb 7H10. C Schematic diagram of alanine mutation of the minimum epitopes identified by mAb 7H10. D The peptides expressed were analyzed by SDS-PAGE Coomassie brilliant blue staining and Western blot with mAb 7H10