Fig. 6.

Characterization of the recognition epitope of mAb 1A2. A Schematic diagram of segment P4-5 further truncation. B SDS-PAGE Coomassie brilliant blue staining and Western blot analysis with mAb 1A2 of the reactivity of segment P4-5 truncated frgment. C Schematic diagram of the B-cell epitope mapping of P523 recognized by mAb 1A2. Two amino acids from the N-terminus or C-terminus were truncated respectively. The red amino acids indicate peptides that reacted with mAb 1A2, while grey amino acids represent peptides that did not react with mAb 1A2. D SDS-PAGE Coomassie brilliant blue staining and Western blot analysis with mAb 1A2. E Schematic diagram of alanine mutation of the minimum epitopes identified by mAb 1A2. F The peptides expressed were analyzed by SDS-PAGE Coomassie brilliant blue staining and Western blot with mAb 1A2