Table 1.
Primers used for NSP4-HR expression and truncation identification
| Primer name | Primer sequence 5 ‘to 3’ |
|---|---|
| 28a-NSP4-F | TAACTTTAAGAAGGAGATATACCATGGGCGCTTTCAGAACTCA |
| 28a-NSP4-R | GTGATGTGATCCCCCTCCTCCCTCGAGTTCCAGTTCGGGTTTGGC |
| HR-F | CAAGGAGGAGGCATTGTCCCTAAGGTCCCGCTCGGT |
| HR-R | ACCGAGCGGGACCTTAGGGACAATGCCTCCTCCTTG |
| 4-1-F | ccacgtgggtccCTGGGATCCGGCGCTTTCAGAACT |
| 4-1-R | CGATGCGGCCGCTCGAGCTACTATGAGTTACCCGTAAG |
| 4-2-F | cacgtgggtccCTGGGATCCGGGAAAATCAAGTGCG |
| 4-2-R | GATGCGGCCGCTCGAGCTACTACCCTTGCCAATTCGG |
| 4-3-F | cacgtgggtccCTGGGATCCGATGTAAAAGGGGACTT |
| 4-3-R | TGCGGCCGCTCGAGCTACTACCCAATAACACCGGgtT |
| 4-4-F | accacgtgggtccCTGGGATCCCGCGCCTATTGGCTG |
| 4-4-R | ATGCGGCCGCTCGAGCTACTATCCTGTGTGAACGCCA |
| 4–5-F | cacgtgggtccCTGGGATCCTCCCCAGTGATTACCG |
| 4–5-R | GATGCGGCCGCTCGAGCTACTACTATTCCAGTTCGGGTTTG |
| 1A2-45-F | GGGCCCCTGGGATCCTCCCCAGTGATTACCGA |
| 1A2-45-1-R | GATGCGGCCGCTCGAGGGTAATCACTGGGGA |
| 1A2-45-2-F | GGGCCCCTGGGATCCGGCGTTCACACAGGA |
| 1A2-45-2-R | GATGCGGCCGCTCGAGTCCTGTGTGAACGCC |
| 7H10-34-F | GGGCCCCTGGGATCCCGCGCCTATTGGCTGA |
| 7H10-34-R | GATGCGGCCGCTCGAGCCCAATAACACCGGgtTCaACGCCA |
| 1A2-5-1-F | CCAGGGGCCCCTGGGATCCTCAAACAAACAAGGAGG |
| 1A2-5-1-R | GTCACGATGCGGCCGCTCGAGTTTAATTATGTGACTG |
| 1A2-5-3-R | CGATGCGGCCGCTCGAGTTCCAGTTCGGGTTTGGCA |
| 1A2-5-2-F | GGGGCCCCTGGGATCCCCTAAGGTCCCGCTCGG |
| 1A2-5-2-R | TCACGATGCGGCCGCTCGAGACAAAGATCTGAAGGC |
| 1A2-5-3-F | GGGGCCCCTGGGATCCGGCAGTCACATAATTAA |
| 1A2-45-F | GGGCCCCTGGGATCCTCCCCAGTGATTACCGA |
| 1A2-45-R | GATGCGGCCGCTCGAGTCCTGTGTGAACGCCAACAAG |
| 7H10-45-C2 | GATGCGGCCGCTCGAGGTGAACGCCAACAAGCTCACCGGCTTCGGTA |
| 7H10-45-C4 | GATGCGGCCGCTCGAGGCCAACAAGCTCACCGGCTTCGGTAATCACT |
| 7H10-45-C6 | GATGCGGCCGCTCGAGAAGCTCACCGGCTTCGGTAATCACTGGGG |
| 7H10-45-C8 | GATGCGGCCGCTCGAGACCGGCTTCGGTAATCACTGGGG |
| 7H10-45-C10 | GATGCGGCCGCTCGAGTTCGGTAATCACTGGGG |
| 7H10-45-N2-F | GGGCCCCTGGGATCCGTGATTACCGA |
| 7H10-45-N2-R | ATGCGGCCGCTCGAGTCCTGTGTGAACGCCAACAAGCTCACCGGCTTCGG |
| 7H10-45-N4-F | GGGGCCCCTGGGATCCACCGAAGCCGGTGAGC |
| 7H10-45-N4-R | ATGCGGCCGCTCGAGTCCTGTGTGAACGCCAACAAGCTCACCGGCTTC |
| 7H10-45-N6-F | GGGGCCCCTGGGATCCGCCGGTGAGC |
| 7H10-45-N6-R | ATGCGGCCGCTCGAGTCCTGTGTGAACGCCAACAAGCTCACCGGC |
| 7H10-45-N8-F | GGGCCCCTGGGATCCGAGCTTGTTGGC |
| 7H10-45-N8-R | GATGCGGCCGCTCGAGTCCTGTGTGAACGCCAACAAGCTC |
| 7H10-45-N10-F | GGGCCCCTGGGATCCGTTGGCGTTCACACAGGA |
| 7H10-45-N10-R | ACGATGCGGCCGCTCGAGTCCTGTGTGAACGCCAAC |
| 7H10-45-C2 | GATGCGGCCGCTCGAGGTGAACGCCAACAAGCTCACCGGCTTCGGTA |
| 1A2-C2-R | ATGCGGCCGCTCGAGTTCGGGTTTGGCAGCAAGCAGGGCACAAAGATCT |
| 1A2-C4-R | ATGCGGCCGCTCGAGTTTGGCAGCAAGCAGGGCACAAAGATCTGAAGGC |
| 1A2-C6-R | ATGCGGCCGCTCGAGAGCAAGCAGGGCACAAAGATCTGAAGGC |
| 1A2-C8-R | ATGCGGCCGCTCGAGCAGGGCACAAAGATCTGAAGGC |
| 1A2-C10-R | ATGCGGCCGCTCGAGACAAAGATCTGAAGGC |
| 1A2-N2-F | GGGGCCCCTGGGATCCTCAGATCTTTGTGCCCTGCTTGCTGCCAAA |
| 1A2-N4-F | GGGGCCCCTGGGATCCCTTTGTGCCCTGCTTGCTGCCAAACCCGAA |
| 1A2-N6-F | GGGGCCCCTGGGATCCGCCCTGCTTGCTGCCAAACCCGAA |
| 1A2-N8-F | GGGGCCCCTGGGATCCCTTGCTGCCAAACCCGAA |
| 1A2-N10-F | GGGGCCCCTGGGATCCGCCAAACCCGAA |
| 1A2-N10-R | CGATGCGGCCGCTCGAGTTCCAGTTCGGGTTT |
| 1A2-C2-R | ATGCGGCCGCTCGAGTTCGGGTTTGGCAGCAAGCAGGGCACAAAGATCTG |
| 1A2-C4-R | ATGCGGCCGCTCGAGTTTGGCAGCAAGCAGGGCACAAAGATCTGAAGGC |
| 1A2-C6-R | ATGCGGCCGCTCGAGAGCAAGCAGGGCACAAAGATCTGAAGGC |
| 1A2-C8-R | ATGCGGCCGCTCGAGCAGGGCACAAAGATCTGAAGGC |
| 1A2-C10-R | ATGCGGCCGCTCGAGACAAAGATCTGAAGGC |
| 1A2-N2-F | GGGGCCCCTGGGATCCTCAGATCTTTGTGCCCTGCTTGCTGCCAAA |
| 1A2-N4-F | GGGGCCCCTGGGATCCCTTTGTGCCCTGCTTGCTGCCAAACCCGAA |
| 1A2-173 A-R | GATGCGGCCGCTCGAGTAGCAGCCAGGGCACAAAGATCTGAAGGCAC |
| 1A2-172 A-R | ATGCGGCCGCTCGAGTAGCAAGAGCGGCACAAAGATCTGAAGGCAC |
| 1A2-170 A-F | GGGCCCCTGGGATCCGTGCCTTCAGATCTTGCT |
| 1A2-170 A-R | ATGCGGCCGCTCGAGTAGCAAGCAGGGCAGCAAGATCTGAA |
| 1A2169A-F | GGGCCCCTGGGATCCGTGCCTTCAGATGCATG |
| 1A2-169 A-R | ATGCGGCCGCTCGAGTAGCAAGCAGGGCACATGCATCTGAAGGCA |
| 1A2-168 A-F | GGGGCCCCTGGGATCCGTGCCTTCAGCTCTTT |
| 1A2-168 A-R | ATGCGGCCGCTCGAGTAGCAAGCAGGGCACAAAGAGCTGAAGGCAC |
| 1A2-165–174-R | ATGCGGCCGCTCGAGTAGCAAGCAGGGCACAAAGATCTGAAGGCA |
| 1A2-167 A-F | GGGGCCCCTGGGATCCGTGCCTGCAGATCTTT |
| 1A2-167-R | ATGCGGCCGCTCGAGTAGCAAGCAGGGCACAAAGATCTGCAGGCA |
| 1A2-166 A-F | GGGGCCCCTGGGATCCGTGGCTTCAGATCTTT |
| 1A2-166 A-R | TGCGGCCGCTCGAGTAGCAAGCAGGGCACAAAGATCTGAAGCCAC |
| 1A2-165 A-F | GGGGCCCCTGGGATCCGCGCCTTCAGATCTTT |
| 1A2-165 A-R | ATGCGGCCGCTCGAGTAGCAAGCAGGGCACAAAGATCTGAAGGCGC |
| 7H10-122–129-F | GATCCGTGATTACCGAAGCCGGTGAGCTT |
| 7H10-122–129-R | TCGAGAAGCTCACCGGCTTCGGTAATCACGGATC |
| 7H10-128-F | GATCCGTGATTACCGAAGCCGGTGCACTT |
| 7H10-128-R | TCGAGAAGTGCACCGGCTTCGGTAATCACGGATC |
| 7H10-129-F | GATCCGTGATTACCGAAGCCGGTGAGGCT |
| 7H10-129-R | TCGAGAGCCTCACCGGCTTCGGTAATCACGGATC |