Skip to main content
PLOS One logoLink to PLOS One
. 2026 Jan 20;21(1):e0340353. doi: 10.1371/journal.pone.0340353

Improving micromorphological analysis with CNN-based segmentation of flint/obsidian, bone and charcoal

Rafael Arnay 1, Pedro García-Villa 2, Javier Hernández-Aceituno 1,*, Sara Rueda-Saiz 2, Carolina Mallol 2
Editor: Przemysław Mroczek3
PMCID: PMC12818656  PMID: 41557713

Abstract

The quantification and identification of components in archaeological micromorphology remain subjective and challenging, particularly for early-career researchers. To address this, we developed a deep learning tool for the automatic segmentation of three materials commonly found in Palaeolithic contexts and thin sections: bone, charcoal, and lithic fine-grained debitage (flint and obsidian). Using high-resolution photomicrographs of 57 thin sections in plane-polarised and cross-polarised light, we trained and evaluated state-of-the-art convolutional neural networks (CNNs) for material segmentation. The best-performing configuration, a U-Net with an InceptionV4 encoder, achieved mean intersection over union (IoU) scores of 0.96 for flint/obsidian, 0.80 for bone, and 0.82 for charcoal. The models also classified the relative abundance of each material with balanced accuracies of 0.99 for flint/obsidian, 0.92 for bone, and 0.85 for charcoal. These results demonstrate the potential of deep learning to enhance objectivity, accuracy, and reproducibility in archaeological micromorphology, providing a valuable resource for future geoarchaeological research.

Introduction

Micromorphology, particularly archaeological micromorphology, where natural and anthropogenic components co-exist, always retains a certain degree of subjectivity. The identification of these components is often challenging, especially for early-career researchers or those without a strong background in mineralogy and soil sciences [1]. Additionally, quantifying the abundance of components in thin section introduces further complexity and subjectivity. Most micromorphologists follow the foundational guidelines established by Bullock et al. (1985) [2], later revised and expanded by Stoops [3]. These general guidelines include a brief section on quantifying component abundance, which has become widely adopted in the field ([3], Fig. 4.12). This method relies on visual estimation using the microscope’s field of view to assess the surface area occupied by each component. Based on this, abundance is categorized as Very Few (<5%), Few (6-15%), Common (16-30%), Frequent (31-50%), Dominant (51-70%) and Very Dominant (>70%). An alternative method, although less commonly used, involves simplified scales that classify abundance as “low”, “medium” or “high”, often represented numerically (e.g., 1. 2. 3) or symbolically (e.g., “+” for low abundance and “+++” for high abundance) [4, 5].

These classification systems, however, are not without issues. As Stoops ([3], p. 57) notes, they are inherently subjective: “The abundancy of fabric units can be expressed in different ways, such as the number of units per surface unit (e.g. the number of quartz grains per square centimeter), or the surface percentage occupied by a partial fabric”. A good example of this ambiguity is the quantification of pedofeatures and porosity features.

For instance, in a thin section dominated by channels, all of which possess limpid clay coatings, it becomes difficult to isolate and measure the coated surfaces precisely. According to Stoops’ framework, the coatings might be classified as Very Few (<5%). However, since most or all of the porosity features are coated, one could argue for a Dominant (51-70%) or even Very Dominant (>70%) classification when considering relative abundance within porosity features.

Given these challenges, some researchers opt to quantify only one or two main components of the sequence, or choose not to quantify abundance at all. Instead, they describe the overall composition of the sample or stratigraphic unit and highlight its key features [6–13]. Others do include quantitative abundance data, either in the main text or in the appendixes [14–20].

Archaeological and geoarchaeological methods are currently advancing at a high pace [21], with the rapid integration of technological and computational advances across all research areas. This evolution is facilitating the development of innovative methodologies as well as the refinement of established ones. It is plausible that, in the near future, much of the data processing in these disciplines will be carried out through machine learning (ML) techniques.

As outlined in the literature, ML and image recognition are beginning to be applied in all observational scales: macro, meso, and micro. At the macro-scale, applications include the detection of archaeological sites [22], predictive modelling for site location [23], resource distribution modelling [24], and site chronology prediction [25]. At the meso-scale, image-based ML has been used to compare manually and computationally conducted traceological analyses [26], often demonstrating high reliability and even increased accuracy in some cases [27]. ML has also been applied in zooarchaeology for the identification and classification of faunal remains [28]. At the micro-scale, ML has been employed in use-wear analysis [29] and micromorphology, for instance, in the identification of porosity features [30], mineral detection [31] and general thin section analysis [32].

To enhance the reliability of micromorphological data, it is essential to maximize objectivity and, where possible, introduce quantitative precision to support interpretation. With this in mind, the primary aim of our project is to develop a tool that uses image-based ML to facilitate the identification and quantification of some of the most common materials in Palaeolithic contexts and thin sections: bone (fresh, degraded, burnt and calcined), charcoal and fine-grained lithic debitage (flint and obsidian).

Materials and methods

This study employs deep learning techniques for semantic segmentation of soil photomicrographs, aiming to detect and differentiate three archaeological components: lithic fine-grained debitage (flint and obsidian), charcoal, and bone remains. Due to an insufficient sample size of flint, we decided to include obsidian fragments in the analysis. Although these materials are different mineralogically and can be distinguished using Crossed Polarized Light (XPL), their conchoideal type of fracture, their shape, size, and optical characteristics under Plane Polarized Light (PPL) are similar. For the sake of consistency, the label flint was retained, although the material under analysis more accurately corresponds to fine-grained lithic debitage. These photomicrographs were obtained from high-resolution scans of 57 micromorphological thin sections from both archaeological and experimental contexts where these materials are abundant. Chronologies and geographical areas include European and Near Eastern Middle Palaeolithic, Iberian Peninsula Neolithic, Ancient Canarian and experimental samples studied in [33].

Of these, 51 were taken under PPL and 6 under XPL. XPL scans were used to address different colours of flint and bone in PPL and XPL. All of the analyzed thin sections are housed at the Archaeological Micromorphology and Biomarkers Research Laboratory (AMBILAB), University of La Laguna, Tenerife. No permits were required for the described study, which complied with all relevant regulations.

Dataset

The dataset is obtained from labeled high-resolution photomicrographs. All input images were segmented and annotated with the LabelMe tool [34], identifying regions corresponding to three structural types: flint/obsidian, bone, charcoal, and background (used when none of the other categories are present). 51 of the thin sections were annotated directly by authors García-Villa and Rueda-Saiz and 6 were annotated by trainees and interns L.M, A.T., A.N and I.M. They were trained and supervised by P.G-V and C.M. and were prepared beforehand with the basics of micromorphology, optical petrography and archaeological remains recognition in thin section. Quality control was assured through assisted annotation and random spot checks.

Both the original images and their corresponding label masks are subdivided into 2000x2000 pixels images. The sub-images that contain at least one pixel corresponding to a given class are included in the dataset for that class. Fig 1 shows some examples of images and their corresponding segmentation masks for each class. In total, there are 820 images for flint/obsidian, 2519 for bone, and 1715 for charcoal. The data are randomly split into training (70%), validation (20%), and test (10%) subsets while ensuring no overlap between the sets. Table 1 shows the number of training, validation and test images per class.

Fig 1. Photomicrographs (left) and labels (right).

Fig 1

Table 1. Number of images in the train, validation and test sets for each class.

Class Train Validation Test
Flint/Obsidian 574 164 82
Bone 1763 503 253
Charcoal 1200 343 172

Each image and mask are resized to a resolution of 512×512 pixels. To increase the diversity of training data, random horizontal and vertical flips are applied as data augmentation techniques using the Albumentations library [35]. Table 2 shows the relative size of regions compared to the image size and number of regions per image for each dataset.

Table 2. Relative size of regions compared to the image size and number of regions per image.

Relative size Number of regions
Class mean std mean std
Flint/Obsidian 0.02 0.16 9.35 9.45
Bone 0.01 0.04 13.87 24.76
Charcoal 0.01 0.05 16.49 20.75

Model architectures and encoders

Modern deep learning-based image segmentation models typically adopt an encoder–decoder architecture, in which the encoder extracts hierarchical features from the input image, and the decoder reconstructs the segmentation map from these features. In this work, we adopt a modular framework where different segmentation heads (e.g., U-Net [36], FPN [37], PSPNet [38]) are combined with various pre-trained convolutional encoders (ResNet [39], VGG [40], Inception [41], Xception [42]).

Encoders.

The encoder, or backbone, is usually derived from a classification network trained on large-scale datasets such as ImageNet. These encoders consist of multiple stages of convolutional and pooling layers that progressively reduce spatial resolution while increasing feature abstraction. To use them for segmentation, the classification head is removed, and the feature maps from different encoder stages are routed to the decoder.

Decoders.

The decoder reconstructs a dense segmentation map from the hierarchical features extracted by the encoder. Each architecture handles this differently:

  • U-Net employs a symmetric structure with skip connections between encoder and decoder layers of equal resolution [36].

  • FPN combines features from different depths in a top-down fashion to handle multi-scale representations [37].

  • PSPNet uses pyramid pooling to incorporate global context at multiple receptive fields [38].

Integration procedure.

The combination of encoder and decoder components proceeds as follows:

  1. Backbone selection: Choose an encoder network and remove its fully connected layers.

  2. Pre-training: Load ImageNet pre-trained weights to leverage transfer learning.

  3. Feature tapping: Extract intermediate feature maps from key layers in the encoder.

  4. Decoder setup: Assemble the decoder to progressively upsample and fuse the feature maps to the original image resolution.

  5. Output head: Apply a final convolutional layer to produce logits in order to calculate the Dice loss.

Loss function and optimisation

A Dice Loss function was used to optimise model performance. The Dice loss is a commonly used metric in semantic segmentation tasks to evaluate the overlap between predicted and ground truth regions [43]. It is derived from the Dice Similarity Coefficient (DSC), and is particularly effective for handling imbalanced class distributions.

Given the predicted segmentation mask P and the ground truth mask G, both represented as binary vectors, the Dice coefficient is defined as:

DSC(P,G)=2∑ipigi∑ipi+∑igi (1)

where pi and gi are the predicted and ground truth values for pixel i, respectively. The Dice loss is then defined as:

ℒDice=1−2∑ipigi+ϵ∑ipi+∑igi+ϵ (2)

Here, ε is a small constant added to avoid division by zero. The Dice loss ranges from 0 (perfect overlap) to 1 (no overlap), and is minimized during training to improve segmentation accuracy. The Adam optimizer [44] was selected with a learning rate of 1×10−4. All models were trained for a maximum of 40 epochs with a batch size of 16.

Model training and evaluation

The training loop monitors the validation Intersection-over-Union (IoU) [45] metric and saves the best-performing model checkpoint for evaluation on the Test set. The IoU, also known as the Jaccard Index, is a standard evaluation metric for measuring the overlap between two regions, typically a predicted segmentation mask P and the ground truth mask G. It is defined as the ratio between the area of intersection and the area of union of the predicted and ground truth regions:

IoU(P,G)=|P∩G||P∪G|=∑ipigi∑ipi+∑igi−∑ipigi (3)

where pi and gi represent the binary values (0 or 1) of the predicted and ground truth masks at pixel i, respectively. The IoU metric ranges from 0 to 1. where a value of 1 indicates perfect overlap, and a value of 0 indicates no overlap. It is widely used in segmentation and object detection benchmarks due to its ability to penalise both false positives and false negatives. Two evaluation strategies were employed:

  • Per-image IoU: Calculates IoU individually for each image and reports the average.

  • Dataset-level IoU: Aggregates pixel-level true positives, false positives, and false negatives over the entire dataset before computing IoU.

Per-image IoU provides insight into the model’s consistency across individual samples, it is more sensitive to local errors, particularly in images with small or sparse target regions. In microscopic archaeological images, these regions – such as tiny fragments of bone, flint/obsidian, or charcoal – may occupy only a minimal portion of the image. As a result, even minor segmentation inaccuracies can cause significant drops in per-image IoU. Moreover, in cases where the target class is nearly absent or fragmented, the metric may be disproportionately penalized, despite acceptable qualitative performance. Conversely, dataset-level IoU tends to smooth out such sample-level fluctuations by emphasizing the overall class distribution and model behaviour across the entire dataset. This makes it a more stable metric for comparative analysis across models, especially when dealing with class imbalance and small object segmentation.

In addition to assessing the system’s performance on the segmentation task, we also aimed to evaluate the system on the classification task, as proposed by [3]. Specifically, this involved classifying the images based on the relative abundance of a given component (bone, charcoal, or flint/obsidian) into five categories: Very Few (<5%), Few (6–15%), Common (16–30%), Frequent (31–50%), Dominant (51–70%), and Very Dominant (>70%). Fig 2 shows a workflow diagram of the segmentation and classification process.

Fig 2. Segmentation and classification workflow.

Fig 2

Results

Tables 3, 4 and 5 summarise the performance of the different segmentation models across target classes using various combinations of architectures and encoders. Evaluation metrics include IoU computed per image and across the entire dataset, for the test sets.

Table 3. Model performance in the bone class.

Architecture Encoder per image IoU dataset IoU
FPN inceptionv4 0.60 0.77
resnet50 0.58 0.75
vgg19 0.62 0.74
xception 0.55 0.75
PSPNet inceptionv4 0.52 0.72
resnet50 0.49 0.71
vgg19 0.56 0.74
xception 0.45 0.65
Unet inceptionv4 0.61 0.80
resnet50 0.60 0.78
vgg19 0.62 0.76
xception 0.61 0.70

Performance of the different segmentation models in the bone class using various combinations of architectures and encoders.

Table 4. Model performance in the charcoal class.

Architecture Encoder per image IoU dataset IoU
FPN inceptionv4 0.59 0.81
resnet50 0.59 0.81
vgg19 0.57 0.80
xception 0.57 0.81
PSPNet inceptionv4 0.49 0.78
resnet50 0.47 0.78
vgg19 0.53 0.80
xception 0.46 0.75
Unet inceptionv4 0.59 0.82
resnet50 0.62 0.81
vgg19 0.59 0.80
xception 0.60 0.82

Performance of the different segmentation models in the charcoal class using various combinations of architectures and encoders.

Table 5. Model performance in the flint/obsidian class.

Architecture Encoder per image IoU dataset IoU
FPN inceptionv4 0.64 0.93
resnet50 0.67 0.92
vgg19 0.68 0.91
xception 0.64 0.95
PSPNet inceptionv4 0.60 0.92
resnet50 0.61 0.89
vgg19 0.66 0.90
xception 0.45 0.85
Unet inceptionv4 0.69 0.96
resnet50 0.67 0.95
vgg19 0.64 0.90
xception 0.71 0.94

Performance of the different segmentation models in the flint/obsidian class using various combinations of architectures and encoders.

Among all configurations, the highest dataset-level IoU was achieved by the U-Net architecture using the InceptionV4 encoder for the flint/obsidian class, reaching an IoU of 0.96. This indicates exceptional segmentation accuracy for identifying flint/obsidian particles in microscopic soil images. FPN combined with Xception also yielded competitive results for the same class (IoU = 0.95).

In general, models targeting flint/obsidian outperformed those aimed at detecting charcoal and bone. The best bone segmentation model (Unet + InceptionV4) reached an IoU of 0.80, while charcoal segmentation peaked at 0.82 with U-Net + InceptionV4 and Xception. Figs 3, 4 and 5 show a qualitative comparison between the ground truth segmentations and those obtained using the Unet + Inceptionv4 model, for bone, charcoal and flint/obsidian, respectively.

Fig 3. Bone class photomicrograph, ground truth, prediction and comparison of the segmentation, where false positives are shown in green and false negatives in red.

Fig 3

Fig 4. Charcoal class photomicrograph, ground truth, prediction and comparison of the segmentation, where false positives are shown in green and false negatives in red.

Fig 4

Fig 5. Flint/obsidian class photomicrograph, ground truth, prediction and comparison of the segmentation, where false positives are shown in green and false negatives in red.

Fig 5

The distribution of abundance categories as described in [3] within the test sets for flint/obsidian, bone, and charcoal is presented in Table 6. This distribution is notably unbalanced across the three structures: for all materials, the “Very Few” category dominates the dataset, particularly for bone (79.05%) and charcoal (64.53%), while flint/obsidian shows a slightly more balanced distribution, though still skewed towards “Very Few” (53.66%). Higher abundance categories such as “Frequent”, “Dominant”, and“Very Dominant” are underrepresented in all three materials, which may pose challenges for the classifier when predicting these less frequent classes.

Table 6. Number and percentage of images per abundance level.

Very Few Few Common Freq. Dom. V.D.
Flint/Obs. 44(53.7%) 19(23.2%) 5(6.1%) 3(3.7%) 5(6.1%) 6(7.3%)
Bone 200(79.1%) 31(12.3%) 16(6.3%) 5(2.0%) 0(0%) 1(0.4%)
Charcoal 111(64.5%) 30(17.4%) 13(7.6%) 8(4.7%) 4(2.3%) 6(3.5%)

Number of images for each abundance level and their corresponding percentage relative to the total number of images in the test set. Obs.: Obsidian, Freq.: Frequent, Dom.: Dominant, V.D.: Very Dominant.

Despite this class imbalance, the classification results using the U-Net model with InceptionV4 as encoder and summarized in Table 7 indicate that the system performs remarkably well across all materials. For flint/obsidian, the model achieves near-perfect performance with a balanced accuracy, precision, recall, and F1-score of 0.99. Bone also demonstrates high classification performance, with a balanced accuracy of 0.92 and an F1-score of 0.94. showing that the model can handle the dominant “Very Few” class as well as the less represented ones. Charcoal presents the lowest performance among the three materials, with a balanced accuracy and F1-score around 0.85 (Fig 6).

Table 7. Classification performance across abundance categories using U-Net with InceptionV4.

Balanced Acc. Precision Recall f1-score
Flint/Obsidian 0.99 0.99 0.99 0.99
Bone 0.92 0.96 0.92 0.94
Charcoal 0.85 0.85 0.85 0.84

Classification performance for the flint/obsidian, bone, and charcoal test sets across abundance categories using the U-Net model with InceptionV4 as encoder. Metrics reported include balanced accuracy, precision (macro averaged), recall (macro averaged), and F1-score (macro averaged).

Fig 6. Confusion matrices of relative abundance classification.

Fig 6

Confusion matrices obtained for the classification of relative abundance using the U-Net model with InceptionV4 as encoder in the test sets for flint/obsidian, charcoal and bone. The categories are: Very Few (<5%), Few (6–15%), Common (16–30%), Frequent (31–50%), Dominant (51–70%), and Very Dominant (>70%).

Discussion

Class performance and methodological considerations

The performance of the models varied substantially across classes, which may reflect intrinsic differences in the visual distinctiveness and morphological consistency of each material class. U-Net generally delivered more robust results than the other architectures. This could be attributed to U-Net’s skip connections, which facilitate precise localisation by preserving spatial resolution, a critical factor when dealing with fine-grained structures like micro-remains.

The variation in encoder performance also reveals interesting trends. InceptionV4 consistently performed well across classes, potentially due to its multi-scale feature extraction capabilities, which are beneficial when segmenting complex textures found in archaeological samples. Interestingly, the dataset-level IoUs were consistently higher than the per-image values, which suggests that even though some individual predictions may underperform, the models perform robustly across the dataset as a whole.

Flint/obsidian consistently achieved the highest accuracy (IoU up to 0.96), reflecting its strong optical distinctiveness under PPL and relatively uniform morphological features. In contrast, bone and charcoal yielded lower IoUs (0.80 and 0.82. respectively). These differences highlight that segmentation quality depends not only on network architecture but also on the inherent variability of each material. It may also reflect the increased difficulty in classifying charcoal, potentially due to more subtle abundance differences or more complex image features.

Bone fragments exhibit heterogeneous textures, colours and shapes, as their preservation state varies from fresh to degraded or burnt, while charcoal often displays a wide range of inner pore variability, based on its species and orientation cut. Such different properties, as well as the annotation difficulties faced (see “Annotating difficulties and future directions” below) likely explain the lower performance observed for these classes.

Typical failure modes further illustrate these challenges. Misclassification of bone often occurred when fragments were small, degraded, or heavily diagenetically altered, producing visual signatures close to the background and other archaeological components. Charcoal errors were frequent in thin or faint fragments, which the model occasionally ignored altogether, biasing predictions toward the dominant “Very Few” category.

Annotation quality also influenced outcomes. All segmentations were produced manually, and even minor inconsistencies in defining boundaries or labeling fine fragments can propagate into training. This effect is compounded by the strong class imbalance in the dataset. Most samples fell into the “Very Few” abundance category, while higher abundance levels (“Frequent”, “Dominant”, “Very Dominant”) were underrepresented. As a result, the models were well-calibrated for sparse occurrences but they may be less reliable when confronted with denser distributions. However, as evidenced by confusion matrices the models are able to predict the most dominant classes quite well.

These performance dynamics have direct implications for applying Stoops’ abundance standards. Automated classifications of “Very Few” or “Few” appear robust for flint/obsidian, but bone and charcoal predictions are less reliable in higher-abundance classes, where underestimation may systematically bias interpretation. In practice, this means that while the system could assist micromorphologists in consistently flagging low-abundance categories, manual verification remains essential when assessing more common or dominant occurrences. For field and laboratory decision-making, this distinction is crucial: overconfidence in model outputs for bone and charcoal could distort reconstructions of combustion features, depositional processes, or occupation intensity.

Finally, there is a methodological risk of data leakage. Although images were randomly split into training, validation, and test sets, thin-section subimages derived from the same slide may share local structures or textures. If such near-duplicates occur across splits, performance estimates could be inflated.

Archaeological impact and applicability

Charcoal, bone, and fine-grained lithic remains are among the most common materials found in thin sections across Palaeolithic contexts. The performance of the model indicates that its application is not restricted to the Palaeolithic but can be extended to any setting where these materials occur. This makes the approach broadly transferable and highly reproducible, since all that is required is a high-resolution scan of the thin section.

This methodology would enhance the quantification of these elements in archaeological features. For instance, in the characterization of combustion structures, where charcoals are frequently found, or the identification of surface occupations and activity areas, characterized by high abundance of bones and fine-grained lithic debitage.

Nevertheless, some challenges remain. Identification might be more difficult when materials have undergone strong taphonomic alteration, causing bones and charcoal to lose their typical colour and morphology under PPL. Likewise, fine-grained debitage can vary in colour depending on raw material, and coarse lithic artefacts without a pronounced conchoidal fracture are difficult to distinguish – both for the CNN and for specialists – from natural rock fragments.

Even so, the archaeological significance of the results is clear. Minor discrepancies in IoU values do not imply that fragments are missed, but rather that the CNN’s boundaries do not align perfectly with the annotated polygons. For example, the lowest-performing case – charcoal identified with the U-Net model and InceptionV4 encoder – still achieved an IoU of 0.84.

Taken together, these outcomes demonstrate the potential impact of the method on archaeological practice. The possibility of automating part of the identification and quantification process constitutes a practical improvement: it would reduce the time micromorphologists spend on manual annotation and counting, while still providing results that are consistent and dependable.

Annotating difficulties and future directions

Human error during the annotation stage proved to be a decisive factor in the final recognition of elements. Several difficulties were identified that should guide future improvements.

LabelMe, although a practical tool, is not the best suited for this task. It only allows polygons built from straight lines, while most bone and charcoal fragments have rounded or curved edges. This mismatch forced the annotation of highly rounded objects to be done with hundreds of tiny segments, making the process slow and often imprecise. By contrast, flint and obsidian – with their sharp edges and angular forms – were far easier to annotate, requiring far fewer steps to achieve a close fit. Very small particles (<50μm) posed another problem: annotating them demanded such high magnification that overall resolution was lost, producing irregular and uncertain boundaries.

Addressing these issues will be central in the next stage of research. The annotation tool itself should be replaced or upgraded with software that handles curved outlines more efficiently, easing the annotation of rounded fragments that dominate the archaeological record. At the same time, the dataset will be expanded to include a greater number of these materials, along with additional fine-grained lithic raw materials such as chert and quartzite, as well as charcoal exhibiting greater morphological variability and bone fragments. Other materials expected to be incorporated into the dataset include pottery fragments, specific rock lithologies, and malacofauna, thereby broadening the applicability of the model.

From a micromorphological standpoint, the next step is to reassemble individual images into full thin sections, so that results can be delivered not only at the scale of the section as a whole, but also at finer levels of analysis—such as defined microfacies or combustion features. These adjustments will improve both the accuracy of annotations and the archaeological relevance of the outputs.

Conclusion

This study presents a successful application of deep learning techniques for the semantic segmentation and abundance classification of archaeological micro-remains in soil thin sections. The U-Net model, particularly when combined with the InceptionV4 encoder, consistently outperformed other configurations across all target materials, achieving high segmentation accuracy, especially for flint/obsidian. The results validate the feasibility of automating material identification in micromorphological images, reducing subjectivity and increasing reproducibility in archaeological analysis. The developed system also proved capable of accurately classifying the relative abundance of materials, even under class imbalance conditions. Although flint/obsidian achieved the highest segmentation and classification performance, bone and charcoal also yielded promising results, suggesting that this approach can be generalized to other micro-remains in archaeological contexts. Future work should focus on expanding the dataset, especially in underrepresented abundance categories, and exploring the application of this methodology to additional materials and archaeological sites.

This study demonstrates the potential of deep learning for segmenting and classifying key archaeological microremains—flint/obsidian, bone, and charcoal—in thin sections. The U-Net with an InceptionV4 encoder achieved high segmentation accuracy, especially for flint/obsidian, and the models were also able to categorise material abundance levels in line with Stoops’ standards. These outcomes suggest that automated approaches can complement traditional micromorphological description by providing consistent quantitative measures that may inform laboratory and field decisions about site formation, combustion features, and occupation evidence.

At the same time, several limitations constrain the generalisability of these results. The dataset derives from a single laboratory collection (57 thin sections), with a strong PPL bias (51 of 57 images), and marked class imbalance—particularly the overrepresentation of “Very Few” abundance categories. The merging of flint and obsidian into a single class reflects the small sample size and raises the risk of conflating distinct materials. Furthermore, several archaeological contexts use chert, quartzite or other fine and coarse-grained materials that are not represented in this study. Split leakage between training and evaluation sets cannot be entirely ruled out. Performance also varied considerably across classes, with excellent results for flint/obsidian but weaker outcomes for bone and charcoal.

Future work should expand the dataset to include more balanced representation of abundance categories and other microremains, incorporate cross-lab validation, and report run-to-run uncertainty. Within those bounds, deep learning has promise as a support tool—rather than a replacement—for micromorphological practice, helping to operationalise Stoops’ abundance classes and guide archaeological interpretation in both laboratory and field contexts.

Acknowledgments

The authors wish to thank L.M for their voluntary participation in tagging the microphotographs, and A.T., A.N and I.M. for their participation in the project within the framework of their bachelor’s internship.

Data Availability

All code created as part of the presented work and the test data used in the experiments are available at the following public repository “CNN-flint-bone-char”, located at https://github.com/jhaceituno/CNN-flint-bone-char.

Funding Statement

The presented work was funded by project “PID2023-150177NB-100 MPHEARTHS”, granted by the “Agencia Estatal de Investigaci’on” of the Government of Spain to co-author Carolina Mallol. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

  • 1.Shahack-Gross R. Archaeological micromorphology self-evaluation exercise. Geoarchaeology. 2016;31(1):49–57. [Google Scholar]
  • 2.Bullock P, Fedoroff N, Jongerius A, Stoops G, Tursina T. Handbook for soil thin section description. Waine Research; 1985. [Google Scholar]
  • 3.Stoops G. Guidelines for analysis and description of soil and regolith thin sections. John Wiley & Sons; 2021. [Google Scholar]
  • 4.Angelucci DE. Geoarchaeology and micromorphology of Abric de la Cativera (Catalonia, Spain). CATENA. 2003;54(3):573–601. doi: 10.1016/S0341-8162(03)00132-2 [DOI] [Google Scholar]
  • 5.Huisman D, Brounen F, Lohof E, Machiels R, Moor J, Van Os B, et al. Micromorphological study of Early Neolithic (LBK) soil features in the Netherlands. Journal of Archaeology in the low Countries. 2014;5.
  • 6.Albert RM, Berna F, Goldberg P. Insights on Neanderthal fire use at Kebara Cave (Israel) through high resolution study of prehistoric combustion features: Evidence from phytoliths and thin sections. Quaternary International. 2012;247:278–93. [Google Scholar]
  • 7.Devos Y, Nicosia C, Vrydaghs L, Modrie S. Studying urban stratigraphy: Dark Earth and a microstratified sequence on the site of the Court of Hoogstraeten (Brussels, Belgium). Integrating archaeopedology and phytolith analysis. Quaternary International. 2013;315:147–66. [Google Scholar]
  • 8.Duarte C, Iriarte E, Diniz M, Arias P. The microstratigraphic record of human activities and formation processes at the Mesolithic shell midden of Poças de São Bento (Sado Valley, Portugal). Archaeological and Anthropological Sciences. 2019;11:483–509. [Google Scholar]
  • 9.Friesem DE, Zaidner Y, Shahack-Gross R. Formation processes and combustion features at the lower layers of the Middle Palaeolithic open-air site of Nesher Ramla, Israel. Quaternary International. 2014;331:128–38. [Google Scholar]
  • 10.Goldberg P, Weiner S, Bar-Yosef O, Xu Q, Liu J. Site formation processes at Zhoukoudian, China. Journal of Human Evolution. 2001;41(5):483–530. [DOI] [PubMed] [Google Scholar]
  • 11.Karkanas P, Shahack-Gross R, Ayalon A, Bar-Matthews M, Barkai R, Frumkin A, et al. Evidence for habitual use of fire at the end of the Lower Paleolithic: site-formation processes at Qesem Cave, Israel. Journal of human evolution. 2007;53(2):197–212. [DOI] [PubMed] [Google Scholar]
  • 12.Nicosia C, Polisca F, Miller C, Ligouis B, Mentzer S, Mangani C, et al. High-resolution sediment analysis reveals Middle Bronze Age byre-houses at the site of Oppeano (Verona province, NE Italy). PLoS One. 2022;17(8):e0272561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Shahack-Gross R, Albert RM, Gilboa A, Nagar-Hilman O, Sharon I, Weiner S. Geoarchaeology in an urban context: the uses of space in a Phoenician monumental building at Tel Dor (Israel). Journal of archaeological science. 2005;32(9):1417–31. [Google Scholar]
  • 14.Fernández-Palacios E, Jambrina-Enrquez M, Mentzer SM, Rodrguez de Vera C, Dinckal A, Égüez N, et al. Reconstructing formation processes at the Canary Islands indigenous site of Belmaco Cave (La Palma, Spain) through a multiproxy geoarchaeological approach. Geoarchaeology. 2023;38(6):713–39. [Google Scholar]
  • 15.Gutiérrez-Rodrguez M, Brassous L, Rodriguez Gutierrez O, Martin Peinado FJ, Orfila M, Goldberg P. Site formation processes and urban transformations during Late Antiquity from a high-resolution geoarchaeological perspective: Baelo Claudia, Spain. Geoarchaeology. 2020;35(2):258–86. [Google Scholar]
  • 16.Kehl M, Marcazzan D, Miller CE, Falcucci A, Duches R, Peresani M. The upper sedimentary sequence of Grotta di Fumane, Northern Italy: a micromorphological approach to study imprints of human occupation and paleoclimate change. Geoarchaeology. 2025;40(2):e70000. [Google Scholar]
  • 17.Mallol C, Cabanes D, Baena J. Microstratigraphy and diagenesis at the upper Pleistocene site of Esquilleu Cave (Cantabria, Spain). Quaternary International. 2010;214(1-2):70–81. [Google Scholar]
  • 18.Ogloblin RamirezI, Galili E, Shahack-Gross R. Underwater Neolithic combustion features: A micro-geoarchaeological study in the submerged settlements off the Carmel Coast, Israel. The Journal of Island and Coastal Archaeology. 2024;19(3):587–609. [Google Scholar]
  • 19.Simpson IA, Milek KB, Gudmundsson G. A reinterpretation of the Great Pit at Hofstadir, Iceland using sediment thin section micromorphology. Geoarchaeology. 1999;14(6):511–30. [Google Scholar]
  • 20.Villagran XS, Strauss A, Miller C, Ligouis B, Oliveira R. Buried in ashes: site formation processes at Lapa do Santo rockshelter, east-central Brazil. Journal of Archaeological Science. 2017;77:10–34. [Google Scholar]
  • 21.Bickler S. Machine learning arrives in archaeology. Advances in Archaeological Practice. 2021;9:186–91. [Google Scholar]
  • 22.Orengo HA, Conesa FC, Garcia-Molsosa A, Lobo A, Green AS, Madella M, et al. Automated detection of archaeological mounds using machine-learning classification of multisensor and multitemporal satellite data. Proceedings of the National Academy of Sciences. 2020;117(31):18240–50. [DOI] [PMC free article] [PubMed]
  • 23.Rafuse DJ. A maxent predictive model for hunter-gatherer sites in the southern pampas, Argentina. Open Quaternary. 2021;7(1). [Google Scholar]
  • 24.Yaworsky PM, Vernon KB, Spangler JD, Brewer SC, Codding BF. Advancing predictive modeling in archaeology: An evaluation of regression and machine learning methods on the Grand Staircase-Escalante National Monument. PloS one. 2020;15(10):e0239424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Klassen S, Weed J, Evans D. Semi-supervised machine learning approaches for predicting the chronology of archaeological sites: A case study of temples from medieval Angkor, Cambodia. PloS one. 2018;13(11):e0205649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Jiménez-Garcia B, Aznarte J, Abellán N, Baquedano E, Dominguez-Rodrigo M. Deep learning improves taphonomic resolution: high accuracy in differentiating tooth marks made by lions and jaguars. Journal of the Royal Society Interface. 2020;17(168):20200446. [Google Scholar]
  • 27.Byeon W, Dominguez-Rodrigo M, Arampatzis G, Baquedano E, Yravedra J, Maté-González MA, et al. Automated identification and deep classification of cut marks on bones and its paleoanthropological implications. Journal of Computational Science. 2019;32:36–43. [Google Scholar]
  • 28.Subirats L, Pérez L, Hernández C, Fort S, Sacha GM. A granularity-based intelligent tutoring system for zooarchaeology. Applied Sciences. 2019;9(22):4960. [Google Scholar]
  • 29.Luncz LV, Braun DR, Marreiros J, Bamford M, Zeng C, Pacome SS, et al. Chimpanzee wooden tool analysis advances the identification of percussive technology. Iscience. 2022;25(11). [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Arnay R, Hernández-Aceituno J, Mallol C. Soil micromorphological image classification using deep learning: the porosity parameter. Applied Soft Computing. 2021;102:107093. [Google Scholar]
  • 31.Nath F, Asish S, Sutradhar S, Li Z, Shahadat N, Debi HR, et al. Rock thin-section analysis, mineral detection utilizing deep learning approach. In: Unconventional Resources Technology Conference and 13–15 June 2023 . Unconventional Resources Technology Conference (URTeC); 2023. p. 3201–11.
  • 32.Zickel M, Gröbner M, Röpke A, Kehl M. MiGIS: micromorphological soil and sediment thin section analysis using an open-source GIS and machine learning approach. E&G Quaternary Science Journal. 2024;73(1):69–93. [Google Scholar]
  • 33.Mallol C, Hernández CM, Cabanes D, Sistiaga A, Machado J, Rodriguez Á, et al. The black layer of Middle Palaeolithic combustion structures. Interpretation and archaeostratigraphic implications. Journal of archaeological science. 2013;40(5):2515–37. [Google Scholar]
  • 34.Russell BC, Torralba A, Murphy KP, Freeman WT. LabelMe: a database and web-based tool for image annotation. International journal of computer vision. 2008;77:157–73. [Google Scholar]
  • 35.Buslaev A, Iglovikov VI, Khvedchenya E, Parinov A, Druzhinin M, Kalinin AA. Albumentations: fast and flexible image augmentations. Information. 2020;11(2):125. [Google Scholar]
  • 36.Ronneberger O, Fischer P, Brox T. U-net: convolutional networks for biomedical image segmentation. In: Medical image computing and computer-assisted intervention–MICCAI 2015 : 18th international conference, Munich, Germany, October 5-9, 2015, proceedings, part III 18. Springer; 2015. p. 234–41.
  • 37.Lin TY, Dollár P, Girshick R, He K, Hariharan B, Belongie S. Feature pyramid networks for object detection. In: Proceedings of the IEEE conference on computer vision and pattern recognition; 2017. p. 2117–25.
  • 38.Zhao H, Shi J, Qi X, Wang X, Jia J. Pyramid scene parsing network. In: Proceedings of the IEEE conference on computer vision and pattern recognition; 2017. p. 2881–90.
  • 39.He K, Zhang X, Ren S, Sun J. Deep residual learning for image recognition. In:Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition (CVPR); 2016. p. 770–8.
  • 40.Simonyan K, Zisserman A. Very deep convolutional networks for large-scale image recognition. arXiv preprint 2014. arXiv:14091556 [Google Scholar]
  • 41.Szegedy C, Ioffe S, Vanhoucke V, Alemi A. Inception-v4, inception-resnet and the impact of residual connections on learning. In: Proceedings of the AAAI conference on artificial intelligence. vol. 31; 2017. p. 1.
  • 42.Chollet F. Xception: deep learning with depthwise separable convolutions. In: Proceedings of the IEEE conference on computer vision and pattern recognition; 2017. p. 1251–8.
  • 43.Milletari F, Navab N, Ahmadi SA. V-net: Fully convolutional neural networks for volumetric medical image segmentation. In: 2016 fourth international conference on 3D vision (3DV). IEEE; 2016. p. 565–71.
  • 44.Kingma DP, Ba J. Adam: a method for stochastic optimization. arXiv preprint . 2014. arXiv:14126980 [Google Scholar]
  • 45.Everingham M, Van Gool L, Williams CK, Winn J, Zisserman A. The Pascal Visual Object Classes (VOC) Challenge. International journal of computer vision. 2010;88(2):303–38. doi: 10.1007/s11263-009-0275-4 [DOI] [Google Scholar]

Decision Letter 0

Przemysław Mroczek

20 Aug 2025

PONE-D-25-43021Improving micromorphological analysis with CNN-based segmentation of flint/obsidian, bone and charcoalPLOS ONE

Dear Dr. Hernández-Aceituno,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Major Revision, grounded in PLOS ONE’s criteria of methodological soundness, transparency and reproducibility rather than novelty; specific content-level comments follow below.

For acceptance, the authors must provide repository DOIs for a minimal, reproducible dataset and all author-generated code, and restructure the manuscript by separating Results from Discussion and tempering the Conclusions; the remaining language, formatting and minor presentation edits are recommended to improve clarity.

Please submit your revised manuscript by Oct 04 2025 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Przemysław Mroczek, Dr. hab.

Academic Editor

PLOS ONE

Journal Requirements:

When submitting your revision, we need you to address these additional requirements.

1. Please ensure that your manuscript meets PLOS ONE's style requirements, including those for file naming. The PLOS ONE style templates can be found at

https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and

https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf

2. In your manuscript, please provide additional information regarding the specimens used in your study. Ensure that you have reported human remain specimen numbers and complete repository information, including museum name and geographic location.

If permits were required, please ensure that you have provided details for all permits that were obtained, including the full name of the issuing authority, and add the following statement:

'All necessary permits were obtained for the described study, which complied with all relevant regulations.'

If no permits were required, please include the following statement:

'No permits were required for the described study, which complied with all relevant regulations.'

For more information on PLOS One's requirements for paleontology and archeology research, see https://journals.plos.org/plosone/s/submission-guidelines#loc-paleontology-and-archaeology-research.

3. Please note that PLOS One has specific guidelines on code sharing for submissions in which author-generated code underpins the findings in the manuscript. In these cases, we expect all author-generated code to be made available without restrictions upon publication of the work. Please review our guidelines at https://journals.plos.org/plosone/s/materials-and-software-sharing#loc-sharing-code and ensure that your code is shared in a way that follows best practice and facilitates reproducibility and reuse.

4. We note that you have indicated that there are restrictions to data sharing for this study. PLOS only allows data to be available upon request if there are legal or ethical restrictions on sharing data publicly. For more information on unacceptable data access restrictions, please see http://journals.plos.org/plosone/s/data-availability#loc-unacceptable-data-access-restrictions.

Before we proceed with your manuscript, please address the following prompts:

a) If there are ethical or legal restrictions on sharing a de-identified data set, please explain them in detail (e.g., data contain potentially identifying or sensitive patient information, data are owned by a third-party organization, etc.) and who has imposed them (e.g., a Research Ethics Committee or Institutional Review Board, etc.). Please also provide contact information for a data access committee, ethics committee, or other institutional body to which data requests may be sent.

b) If there are no restrictions, please upload the minimal anonymized data set necessary to replicate your study findings to a stable, public repository and provide us with the relevant URLs, DOIs, or accession numbers. For a list of recommended repositories, please see

https://journals.plos.org/plosone/s/recommended-repositories. You also have the option of uploading the data as Supporting Information files, but we would recommend depositing data directly to a data repository if possible.

We will update your Data Availability statement on your behalf to reflect the information you provide.

5. If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise.

Additional Editor Comments:

Having read the manuscript carefully—and as archaeological micromorphology is close to my area of expertise—I consider that the submission requires both substantive and technical revisions before it can proceed to external review. It is difficult to recruit a specialist reviewer for a paper that visibly needs structural and policy-aligned polishing; therefore, I am listing here only the content-level points that must be addressed first.

Title. Appropriate and broadly aligned with the study’s scope; no change required at this stage.

Abstract. Needs tightening and rebalancing. Please use British spelling (Palaeolithic), expand abbreviations at first mention (e.g., intersection over union (IoU)), and avoid overloading the abstract with architecture names—retain a generic “state-of-the-art CNNs” and, if essential, the single best configuration. The abstract currently foregrounds only the top metric (IoU = 0.96 for flint/obsidian); include representative values for bone and charcoal to avoid selective emphasis. Add minimal dataset context (e.g., number of thin sections, imaging modes) and provide at least one numeric value to substantiate the claim of “high balanced accuracy” for abundance classification. Stylistically, “flint (including obsidian)” reads more clearly than “flint/obsidian”.

Keywords. Streamline for discoverability. Avoid duplicating title phrases (e.g., “CNN-based”, “semantic segmentation”, “archaeological micromorphology”), avoid acronyms, standardise to UK spelling and lower case for common nouns, and remove slash forms. Keep a concise set focused on the paper’s domain/material scope without repeating title terms.

Structure (Results vs Discussion). Please separate Results and Discussion into distinct, substantive sections. The current combined section mainly reports metrics with limited interpretation, which blurs what was found versus what those findings mean. Results should be strictly descriptive (dataset composition, training/evaluation set-up, per-class/per-image performance, ablations, error rates). A standalone Discussion should interpret performance differences between classes, typical failure modes and their causes, the influence of annotation quality and class imbalance, risks of data leakage, and the extent of generalisability; situate outcomes within the literature, state limitations and potential biases, and outline practical implications for archaeological micromorphology and directions for future work.

Conclusion. Serviceable as a brief recap but currently too general and overly optimistic relative to the dataset’s constraints. The headline claim that the approach “reduces subjectivity and increases reproducibility” is not demonstrated: code and data are not yet public and validation is narrow. The Conclusion should explicitly state limitations and the bounds of generalisability (single-lab, 57 thin sections, strong PPL bias, pronounced class imbalance), and qualify any extension to “other microremains”. Assertions about reproducibility should be conditional on releasing the minimal dataset and code, with transparent train/validation/test splits and fixed random seeds. Please articulate the practical “so what?” (how segmentations and abundance classes map onto Stoops’ standards and support laboratory/field decisions) and acknowledge key methodological risks (merging flint with obsidian due to small sample size; potential split leakage; absence of uncertainty estimates across runs). The tone should be tempered to reflect disparate class performance—excellent for flint/obsidian, clearly lower for bone and charcoal—so that conclusions align with the evidence presented.

Selected technical/clarity items (for the next revision).

– Several tables use comma decimal separators (e.g., “0,60”) rather than the journal’s conventional full stop (e.g., “0.60”), and one caption truncation is visible (“Freqe”). Please standardise decimals and fix caption typos.

– Ensure consistent British spelling throughout (e.g., behaviour, organisation, optimisation). There are a few typographical slips (e.g., “structed” → “structured”).

– You note use of LabelMe and acknowledge volunteers/interns for tagging. Please add brief details on who annotated, any training, and quality control (e.g., spot checks, inter-annotator agreement), as these directly affect dataset validity.

– The dataset comprises scans of 57 thin sections curated at AMBILAB (University of La Laguna). Clarify ownership of the images and permissions to redistribute them. If the institution owns the images, please secure permission for public sharing; if not, detail the rights holder and the access mechanism.

Please revise the manuscript in line with the substantive points above. When resubmitting, include (i) a point-by-point response to every item, (ii) a clean manuscript and a marked version showing changes, and (iii) repository DOIs for the minimal dataset and author-generated code together with an updated, reader-facing Data Availability Statement. I will reassess the submission for external peer review under a decision of Major Revision once these materials are provided.

[Note: HTML markup is below. Please do not edit.]

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

PLoS One. 2026 Jan 20;21(1):e0340353. doi: 10.1371/journal.pone.0340353.r002

Author response to Decision Letter 1


23 Sep 2025

The authors greatly appreciate the provided suggestions, which have been addressed as described below:

- The style requirements for PLOS ONE have been applied to the manuscript, including authors information, figure citations, section title capitalisation, and file naming.

- Additional information regarding the permits required for our study has been included in lines 107-109 of the manuscript

- A code and data availability statement has been included at the end of the manuscript. Legal restrictions over the experimental dataset have been lifted and it is now freely available along with the code.

- The manuscript has been corrected to use British spelling and the abstract has been modified to expand all abbreviations, to avoid unnecessary architecture names, and to include representative values for bone and charcoal, minimal dataset context, and numeric values to substantiate the claim of high balanced accuracy for abundance classification. As recommended, ocurrences of "flint/obsidian" have been replaced with "flint (including obsidian)".

- Keywords have been modified to avoid duplicating title phrases, as well as acronyms and slash forms.

- The "Results" and "Discussion" sections have been separated into distinct, substantive sections. As suggested, the newly added "Discussion" section includes an interpretation of performance differences between classes, typical failure modes and their causes, the influence of annotation quality and class imbalance, risks of data leakage (subsection "Class performance and methodological considerations" and further extended in subsection "Annotating difficulties and future directions"); the extent of generalisability, the limitations and potential biases of the study, and outline practical implications for archaeological micromorphology and directions for future work (subsection "Archaeological impact and applicability").

- The conclusion section has been expanded to explicitly state the limitations and the bounds of generalisability of the presented study. As explained in the newly added "Data availabilty statement", both the code and all experimental data are now publicly available. Key methodological risks have been acknowledged and the tone of the text has been adjusted to align with the evidence presented.

- Incorrect decimal separators and table captions have been fixed.

- To the best of our knowledge, all inconsistent spelling ("optimise", "penalise", "summarise"...) and typographical mistakes have been corrected.

- Specific information regarding image tagging duties has been added to the "Dataset" subsection of "Materials and methods".

Attachment

Submitted filename: rebuttal.pdf

pone.0340353.s001.pdf (43.1KB, pdf)

Decision Letter 1

Przemysław Mroczek

18 Nov 2025

PONE-D-25-43021R1Improving micromorphological analysis with CNN-based segmentation of flint/obsidian, bone and charcoalPLOS ONE

Dear Dr. Hernández-Aceituno,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

The single external review obtained for this revision was clearly positive and raised only minor points requiring clarification. My own re-evaluation confirms that the manuscript is technically sound, adheres to PLOS ONE’s data and methodological transparency requirements, and has been substantially improved in response to both the reviewer’s comments and the initial editorial screening. The remaining issues concern only brief clarifications regarding material classification and sample provenance, which are required for acceptance.

Please submit your revised manuscript by Jan 02 2026 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

  • A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.

  • A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.

  • An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Przemysław Mroczek, Dr. hab.

Academic Editor

PLOS ONE

Journal Requirements:

If the reviewer comments include a recommendation to cite specific previously published works, please review and evaluate these publications to determine whether they are relevant and should be cited. There is no requirement to cite these works unless the editor has indicated otherwise.

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Additional Editor Comments:

Dear Authors,

Thank you very much for submitting the revised version of your manuscript and for your careful and constructive responses to the comments raised during the evaluation process. One external review was obtained for this revision, and the reviewer was clearly positive regarding the scientific soundness, methodological transparency, data availability and clarity of presentation. The reviewer recommended only minor revisions and highlighted that the manuscript is well structured, innovative in its application of CNN-based segmentation to micromorphological materials, and valuable for improving reproducibility in archaeological micromorphology. I appreciate the considerable effort you have made to address both the reviewer’s suggestions and the earlier editorial requirements.

I have re-evaluated the revised manuscript in full, including the supplementary materials and the publicly accessible code and dataset repositories, and I also conducted an internal check of consistency, terminology and compliance with PLOS ONE policy. The manuscript has improved substantially, and in its present form it is close to being suitable for acceptance. Before proceeding, however, I kindly request that you address two minor issues in a final revision. First, although you explain why flint and obsidian were merged into a single class, it would be helpful to state explicitly whether the model performs equally well on both materials and whether this merging was driven exclusively by sample size or also by their practical indistinguishability in PPL/XPL imagery. This brief clarification, which the reviewer also noted, will be useful for future users of the dataset. Second, the manuscript currently describes the thin sections as originating from “different contexts, regions and time periods”. For transparency and archaeological replicability, please provide one additional clarifying sentence indicating at least the broad geographic areas or approximate chronological ranges represented in the dataset. This does not require detailed provenance information, merely a minimal contextual indication.

I look forward to receiving your revised version.

With best regards,

Przemysław Mroczek

Academic Editor

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: (No Response)

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript “Improving micromorphological analysis with CNN-based segmentation of flint/obsidian, bone and charcoal” presents an innovative application of CNNs to archaeological micromorphology. Overall, the study demonstrates the potential of deep learning for improving reproducibility and consistency in archaeological micromorphology, while also acknowledging dataset limitations, class imbalance, and annotation challenges. The work is clearly structured, the dataset and methods are transparent, and the results are promising. I appreciate the effort to make code and data openly available. However, there are aspects that would benefit from clarification and reorganisation, mainly concerning consistency in terminology, the structure of the introduction and results, and the archaeological contextualisation.

Major comments

Abstract

The phrase “(…) flint (including obsidian)” suggests that obsidian is a subtype of flint. Please rephrase to clarify that they were clustered together for methodological reasons, not because of material taxonomy. Correct it throughout.

Moreover, are flint and obsidian truly indistinguishable in this approach? Is the model equally reliable with both, or could there be differences? Since not all Palaeolithic contexts contain obsidian, and many use other types of chert, this limitation should be acknowledged.

Introduction

Lines 14-17: add references.

Lines 22-23: explain the statement and cite if not your own reasoning.

Lines 24-25: this does not need a new paragraph.

Lines 35-51: this is the core of the introduction, but it is currently diffuse. Please rewrite to highlight clearly the aim, how, and significance of the study.

Lines 44-48: add references.

Lines 52-58: the tone is too didactic; I suggest trimming or rephrasing. The last paragraph of the introduction should state succinctly: what you propose, why it matters, and what novelty it brings to the field.

Related work

As written, this section feels isolated. Consider integrating the discussion of ML applications into Introduction, Methods, or Discussion instead of leaving it standalone.

Lines 66-74 (Big Data vs Slow Data): this paragraph is disconnected. If you keep it, please explain why this distinction matters. Line 71 needs a reference.

Materials and methods

In Fig. 1 use the same terminology (Flint/Obsidian) as in the text.

Table 2: unify the number format and apply a consistent rounding rule across rows.

Line 183: no need to re-explain the acronym after the first mention; please correct throughout.

Please provide more detail about the origin of contexts and the experimental samples: where do they come from, and how were they created?

Results

Table 6: write Flint/obsidian or explain the abbreviation.

This section contains methodological notes and interpretive comments that should be moved to Methods and/or Discussion. The Results section should be reserved for data only.

Lines 273-275: this partly duplicates the caption of Fig. 5. Please integrate or remove repetition.

Discussion

Lines 339-341: the phrasing is too promotional; please reword to avoid sensational tone.

The archaeological impact would benefit from more concrete examples: how exactly would this tool improve interpretation of hearth features, stratigraphic complexity, or activity areas?

The limitations section should note that charcoal, in particular, requires more samples and more morphological variability to improve training.

Other comments

In figure titles, you can avoid phrasing such as “Results of …”.

Avoid one-sentence paragraphs throughout the text; merge with adjacent ones for smoother reading.

A workflow figure (dataset → annotation → model training → results) would improve accessibility.

Standardise terminology: Flint/Obsidian throughout.

Ensure all abbreviations are expanded at first use (IoU, PPL, XPL) and no need to expand them in the following occurrences (e.g., line 183).

Improve figure/table captions so they are more self-explanatory.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

**********

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

To ensure your figures meet our technical requirements, please review our figure guidelines: https://journals.plos.org/plosone/s/figures

You may also use PLOS’s free figure tool, NAAS, to help you prepare publication quality figures: https://journals.plos.org/plosone/s/figures#loc-tools-for-figure-preparation.

NAAS will assess whether your figures meet our technical requirements by comparing each figure against our figure specifications.

PLoS One. 2026 Jan 20;21(1):e0340353. doi: 10.1371/journal.pone.0340353.r004

Author response to Decision Letter 2


18 Dec 2025

The authors greatly appreciate the provided suggestions, which have been addressed as described below:

- The phrase "(...) flint (including obsidian)" suggests that obsidian is a subtype of flint. Please rephrase to clarify that they were clustered together for methodological reasons, not because of material taxonomy. Correct it throughout.}

The text has been rephrased to "lithic fine-grained debitage (flint and obsidian)" and corrected throughout the whole manuscript.

- Moreover, are flint and obsidian truly indistinguishable in this approach? Is the model equally reliable with both, or could there be differences? Since not all Palaeolithic contexts contain obsidian, and many use other types of chert, this limitation should be acknowledged.

The following sentence has been included in "Annotating difficulties and future directions" and "Conclusions" respectively: "the dataset will be expanded to include a greater number of these materials, along with additional fine-grained lithic raw materials such as chert and quartzite," and "Furthermore, several archaeological contexts use chert, quartzite or other fine and coarse-grained materials that are not represented in this study."

= Introduction:

- Lines 14--17: add references.

References Angelucci (2003) and Huisman et al. (2014) were added to the paragraph.

- Lines 22--23: explain the statement and cite if not your own reasoning.

- Lines 24--25: this does not need a new paragraph.}

The statement was merged with the next paragraph, where it is explained.

- Lines 35-51: this is the core of the introduction, but it is currently diffuse. Please rewrite to highlight clearly the aim, how, and significance of the study.

To enhance the clarity of the aim and objectives of the article, we propose to merge the two paragraphs into one: "To enhance the reliability of micromorphological data, it is essential to maximize objectivity and, where possible, introduce quantitative precision to support interpretation. With this in mind, the primary aim of our project is to develop a tool using image--based ML that facilitates the identification and quantification of some of the most common materials in Palaeolithic contexts and thin sections: bone (fresh, degraded, burnt and calcined), charcoal and fine-grained lithic debitage (flint and obsidian)."

- Lines 44-48: add references.

As suggested in the response above, these paragraphs have been removed.

- Lines 52-58: the tone is too didactic; I suggest trimming or rephrasing. The last paragraph of the introduction should state succinctly: what you propose, why it matters, and what novelty it brings to the field.

The paragraph has been removed. The introduction now finalizes with stating the aims of the article.

= Related work

- As written, this section feels isolated. Consider integrating the discussion of ML applications into Introduction, Methods, or Discussion instead of leaving it standalone.

The paragraph has been moved to the introduction, before the "aims" paragraph.

- Lines 66-74 (Big Data vs Slow Data): this paragraph is disconnected. If you keep it, please explain why this distinction matters. Line 71 needs a reference.

The paragraph "Big Data vs Slow Data" has been removed, as well as lines 86-93 as they felt disconnected.

= Materials and methods

- In Fig. 1 use the same terminology (Flint/Obsidian) as in the text.

The legend of figure 1 has been modified to match the terminology of the rest of the work.

- Table 2: unify the number format and apply a consistent rounding rule across rows.

All numeric data in table 2 have been unified to fixed format with two decimal digits.

- Line 183: no need to re-explain the acronym after the first mention; please correct throughout.

All redefinitions of the IoU acronym have been removed, except the first instance and within the abstract.

- Please provide more detail about the origin of contexts and the experimental samples: where do they come from, and how were they created?

The following sentence has been included for contextual indication, following the editor's comments: "These photomicrographs were obtained from [...] contexts where these materials are abundant. Chronologies and geographical areas include European and Near Eastern Middle Palaeolithic, Iberian Peninsula Neolithic and Ancient Canarian and experimental samples studied in Mallol et al. (2013)."

= Results

- Table 6: write Flint/obsidian or explain the abbreviation.

An explanation of the abbreviation has been included in the caption of Table 6.

- This section contains methodological notes and interpretive comments that should be moved to Methods and/or Discussion. The Results section should be reserved for data only.

The second paragraph of the Results section has been partially removed, as its information was already discussed in the "Materials and Methods" section. Similarly, several paragraphs discussing the meaning of the results have been moved to the beginning of the Discussion section.

- Lines 273-275: this partly duplicates the caption of Fig. 5. Please integrate or remove repetition.

The redundant paragraph has been removed in favor of the figure caption.

= Discussion

- Lines 339-341: the phrasing is too promotional; please reword to avoid sensational tone.

The sentence has been reworded as "The possibility of automating part of the identification and quantification process constitutes a practical improvement: it would reduce the time micromorphologists spend on manual annotation and counting, while still providing results that are consistent and dependable."

- The archaeological impact would benefit from more concrete examples: how exactly would this tool improve interpretation of hearth features, stratigraphic complexity, or activity areas?

The following paragraph was added: "This methodology would enhance the quantification of these elements in archaeological features. For instance, in the characterization of combustion structures, where charcoals are frequently found, or the identification of surface occupations and activity areas, characterized by high abundance of bones and fine-grained lithic debitage."

- The limitations section should note that charcoal, in particular, requires more samples and more morphological variability to improve training.

The paragraph was rephrased as follows: "At the same time, the dataset will be expanded to include a greater number of these materials, along with additional fine-grained lithic raw materials such as chert and quartzite, as well as charcoal exhibiting greater morphological variability and bone fragments. Other materials expected to be incorporated into the dataset include pottery fragments, specific rock lithologies, and malacofauna, thereby broadening the applicability of the model."

= Other comments

- In figure titles, you can avoid phrasing such as "Results of ...".

The captions of figures 2, 3 and 4 have been simplified by removing the redundant wording.

- Avoid one--sentence paragraphs throughout the text; merge with adjacent ones for smoother reading.

To improve readability, all one-sentence paragraphs have been merged into larger bodies, as marked by their initial letters in the highlighted manuscript, or removed.

- A workflow figure (dataset → annotation → model training → results) would improve accessibility.

A workflow figure has been added at the end of the Materials and Methods section, as suggested.

- Standardise terminology: Flint/Obsidian throughout.

All occurences of the term have been standardised in the manuscript.

- Ensure all abbreviations are expanded at first use (IoU, PPL, XPL) and no need to expand them in the following occurrences (e.g., line 183).

All abbreviations have been fixed, expanding them only on their first appearance.

- Improve figure/table captions so they are more self-explanatory.

The captions of tables 6 and 7 have been reworded to be more self-explanatory.

Attachment

Submitted filename: rebuttal_auresp_2.pdf

pone.0340353.s002.pdf (74.3KB, pdf)

Decision Letter 2

Przemysław Mroczek

21 Dec 2025

Improving micromorphological analysis with CNN-based segmentation of flint/obsidian, bone and charcoal

PONE-D-25-43021R2

Dear Dr. Hernández-Aceituno,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice will be generated when your article is formally accepted. Please note, if your institution has a publishing partnership with PLOS and your article meets the relevant criteria, all or part of your publication costs will be covered. Please make sure your user information is up-to-date by logging into Editorial Manager at Editorial Manager® and clicking the ‘Update My Information' link at the top of the page. For questions related to billing, please contact billing support.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Przemysław Mroczek, Dr. hab.

Academic Editor

PLOS One

Additional Editor Comments (optional):

Dear Dr. Hernández-Aceituno,

thank you for submitting the revised version of your manuscript, “Improving micromorphological analysis with CNN-based segmentation of flint/obsidian, bone and charcoal” (PONE-D-25-43021R2), to PLOS ONE.

I have now completed my editorial assessment of the revised manuscript and the authors’ responses to the previous comments. I am pleased to inform you that your manuscript is accepted for publication in PLOS ONE.

The revised version adequately and clearly addresses all substantive points raised during the review process. In particular, the manuscript now demonstrates improved focus and structure, a more balanced and appropriate tone in the Discussion, clearer articulation of methodological choices and limitations, and full compliance with PLOS ONE’s policies on data availability, transparency, and reporting. The study meets the journal’s criteria for methodological soundness and reproducibility, and it represents a valuable contribution to the development of quantitative and computational approaches in archaeological micromorphology.

Before the manuscript proceeds to production, please note the following minor editorial observations, which are offered for your information only and do not require a revised submission or formal response:

-The Conclusion section contains some thematic overlap between consecutive paragraphs; light streamlining during copyediting may further improve clarity.

-Where applicable, copyediting may slightly harmonise phrasing related to model limitations and generalisability, without altering the substance of your conclusions.

These points will be handled, as appropriate, during the production and copyediting stages.

I thank you for submitting your work to the journal and for your careful and constructive engagement throughout the review process. I look forward to seeing your article published.

Kind regards,

Przemysław Mroczek, Dr hab.

Academic Editor

Reviewers' comments:

Acceptance letter

Przemysław Mroczek

PONE-D-25-43021R2

PLOS One

Dear Dr. Hernández-Aceituno,

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS One. Congratulations! Your manuscript is now being handed over to our production team.

At this stage, our production department will prepare your paper for publication. This includes ensuring the following:

* All references, tables, and figures are properly cited

* All relevant supporting information is included in the manuscript submission,

* There are no issues that prevent the paper from being properly typeset

You will receive further instructions from the production team, including instructions on how to review your proof when it is ready. Please keep in mind that we are working through a large volume of accepted articles, so please give us a few days to review your paper and let you know the next and final steps.

Lastly, if your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

You will receive an invoice from PLOS for your publication fee after your manuscript has reached the completed accept phase. If you receive an email requesting payment before acceptance or for any other service, this may be a phishing scheme. Learn how to identify phishing emails and protect your accounts at https://explore.plos.org/phishing.

If we can help with anything else, please email us at customercare@plos.org.

Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Dr. hab. Przemysław Mroczek

Academic Editor

PLOS One

Associated Data

    This section collects any data citations, data availability statements, or supplementary materials included in this article.

    Supplementary Materials

    Attachment

    Submitted filename: rebuttal.pdf

    pone.0340353.s001.pdf (43.1KB, pdf)
    Attachment

    Submitted filename: rebuttal_auresp_2.pdf

    pone.0340353.s002.pdf (74.3KB, pdf)

    Data Availability Statement

    All code created as part of the presented work and the test data used in the experiments are available at the following public repository “CNN-flint-bone-char”, located at https://github.com/jhaceituno/CNN-flint-bone-char.


    Articles from PLOS One are provided here courtesy of PLOS

    RESOURCES