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. 1995 Apr;68(4 Suppl):271S–274S.

Determinants of motor polarity in the kinesin proteins.

S A Endow 1
PMCID: PMC1281944  PMID: 7787089

Abstract

Many of the proteins that are members of the kinesin family of microtubule motor proteins are plus-end motors; however, a few of the kinesin proteins have now been found to be minus-end microtubule motors. Overall structural features of the proteins can be used to identify further kinesins that are likely to be minus-end motors. Structural or biochemical differences that may serve as the basis of the "reversed" polarity of a unique subset of the kinesin proteins are discussed.

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Selected References

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  1. Ando A., Kikuti Y. Y., Kawata H., Okamoto N., Imai T., Eki T., Yokoyama K., Soeda E., Ikemura T., Abe K. Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region. Immunogenetics. 1994;39(3):194–200. doi: 10.1007/BF00241260. [DOI] [PubMed] [Google Scholar]
  2. Bloom G. S., Endow S. A. Motor proteins. 1: kinesins. Protein Profile. 1994;1(10):1059–1116. [PubMed] [Google Scholar]
  3. Chandra R., Salmon E. D., Erickson H. P., Lockhart A., Endow S. A. Structural and functional domains of the Drosophila ncd microtubule motor protein. J Biol Chem. 1993 Apr 25;268(12):9005–9013. [PubMed] [Google Scholar]
  4. Endow S. A., Chandra R., Komma D. J., Yamamoto A. H., Salmon E. D. Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle pole defects in mitosis. J Cell Sci. 1994 Apr;107(Pt 4):859–867. doi: 10.1242/jcs.107.4.859. [DOI] [PubMed] [Google Scholar]
  5. Endow S. A., Kang S. J., Satterwhite L. L., Rose M. D., Skeen V. P., Salmon E. D. Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J. 1994 Jun 1;13(11):2708–2713. doi: 10.1002/j.1460-2075.1994.tb06561.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilbert S. P., Johnson K. A. Pre-steady-state kinetics of the microtubule-kinesin ATPase. Biochemistry. 1994 Feb 22;33(7):1951–1960. doi: 10.1021/bi00173a044. [DOI] [PubMed] [Google Scholar]
  7. Goodson H. V., Kang S. J., Endow S. A. Molecular phylogeny of the kinesin family of microtubule motor proteins. J Cell Sci. 1994 Jul;107(Pt 7):1875–1884. doi: 10.1242/jcs.107.7.1875. [DOI] [PubMed] [Google Scholar]
  8. Hackney D. D. Kinesin ATPase: rate-limiting ADP release. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6314–6318. doi: 10.1073/pnas.85.17.6314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hackney D. D. The rate-limiting step in microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains occurs while bound to the microtubule. J Biol Chem. 1994 Jun 10;269(23):16508–16511. [PubMed] [Google Scholar]
  10. Harrison B. C., Marchese-Ragona S. P., Gilbert S. P., Cheng N., Steven A. C., Johnson K. A. Decoration of the microtubule surface by one kinesin head per tubulin heterodimer. Nature. 1993 Mar 4;362(6415):73–75. doi: 10.1038/362073a0. [DOI] [PubMed] [Google Scholar]
  11. Hatsumi M., Endow S. A. Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis. J Cell Sci. 1992 Mar;101(Pt 3):547–559. doi: 10.1242/jcs.101.3.547. [DOI] [PubMed] [Google Scholar]
  12. Hatsumi M., Endow S. A. The Drosophila ncd microtubule motor protein is spindle-associated in meiotic and mitotic cells. J Cell Sci. 1992 Dec;103(Pt 4):1013–1020. doi: 10.1242/jcs.103.4.1013. [DOI] [PubMed] [Google Scholar]
  13. Huang T. G., Hackney D. D. Drosophila kinesin minimal motor domain expressed in Escherichia coli. Purification and kinetic characterization. J Biol Chem. 1994 Jun 10;269(23):16493–16501. [PubMed] [Google Scholar]
  14. Huang T. G., Suhan J., Hackney D. D. Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coli. J Biol Chem. 1994 Jun 10;269(23):16502–16507. [PubMed] [Google Scholar]
  15. Kimble M., Church K. Meiosis and early cleavage in Drosophila melanogaster eggs: effects of the claret-non-disjunctional mutation. J Cell Sci. 1983 Jul;62:301–318. doi: 10.1242/jcs.62.1.301. [DOI] [PubMed] [Google Scholar]
  16. Kirchner K., Mandelkow E. M. Tubulin domains responsible for assembly of dimers and protofilaments. EMBO J. 1985 Sep;4(9):2397–2402. doi: 10.1002/j.1460-2075.1985.tb03945.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lockhart A., Cross R. A. Origins of reversed directionality in the ncd molecular motor. EMBO J. 1994 Feb 15;13(4):751–757. doi: 10.1002/j.1460-2075.1994.tb06317.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McDonald H. B., Stewart R. J., Goldstein L. S. The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor. Cell. 1990 Dec 21;63(6):1159–1165. doi: 10.1016/0092-8674(90)90412-8. [DOI] [PubMed] [Google Scholar]
  19. Mitsui H., Yamaguchi-Shinozaki K., Shinozaki K., Nishikawa K., Takahashi H. Identification of a gene family (kat) encoding kinesin-like proteins in Arabidopsis thaliana and the characterization of secondary structure of KatA. Mol Gen Genet. 1993 Apr;238(3):362–368. doi: 10.1007/BF00291995. [DOI] [PubMed] [Google Scholar]
  20. O'Connell M. J., Meluh P. B., Rose M. D., Morris N. R. Suppression of the bimC4 mitotic spindle defect by deletion of klpA, a gene encoding a KAR3-related kinesin-like protein in Aspergillus nidulans. J Cell Biol. 1993 Jan;120(1):153–162. doi: 10.1083/jcb.120.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Song Y. H., Mandelkow E. Recombinant kinesin motor domain binds to beta-tubulin and decorates microtubules with a B surface lattice. Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1671–1675. doi: 10.1073/pnas.90.5.1671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stewart R. J., Thaler J. P., Goldstein L. S. Direction of microtubule movement is an intrinsic property of the motor domains of kinesin heavy chain and Drosophila ncd protein. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5209–5213. doi: 10.1073/pnas.90.11.5209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Taylor E. W. Cell motility. Variations on the theme of movement. Nature. 1993 Jan 14;361(6408):115–116. doi: 10.1038/361115a0. [DOI] [PubMed] [Google Scholar]
  24. Wald H. Cytologic Studies on the Abnormal Development of the Eggs of the Claret Mutant Type of Drosophila Simulans. Genetics. 1936 May;21(3):264–281. doi: 10.1093/genetics/21.3.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Walker R. A., Salmon E. D., Endow S. A. The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature. 1990 Oct 25;347(6295):780–782. doi: 10.1038/347780a0. [DOI] [PubMed] [Google Scholar]
  26. Yang J. T., Saxton W. M., Stewart R. J., Raff E. C., Goldstein L. S. Evidence that the head of kinesin is sufficient for force generation and motility in vitro. Science. 1990 Jul 6;249(4964):42–47. doi: 10.1126/science.2142332. [DOI] [PubMed] [Google Scholar]

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