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. 1978 May;278:287–296. doi: 10.1113/jphysiol.1978.sp012304

Changes in plasma dopamine beta-hydroxylase activity induced by stimulation of the complete sympathetic outflow in the pithed rat.

A G García, F Pelayo, P Sánchez-García
PMCID: PMC1282349  PMID: 671301

Abstract

1. Plasma dopamine beta-hydroxylase (DBH) activity, noradrenaline tissue levels and blood pressure were monitored in the pithed rat following electrical stimulation of the complete sympathetic outflow of the spinal cord. Stimulation at 10 or 30 Hz evoked marked increases in mean blood pressure which averaged 121 mmHg and were abolished by phenoxybenzamine. 2. Stimulation for 1 hr at 10 or 30 Hz did not change the noradrenaline content of the spleen nor the catecholamine content of the adrenals, but the heart noradrenaline content was doubled. 3. Plasma DBH activity was increased by 27% after 1 hr stimulation at 30 Hz, but remained unchanged after stimulation at 10 Hz, or at 30 Hz in phenoxybenzamine-treated rats. 4. We conclude that the pressor responses evoked by sympathetic nerve stimulation are due to the release of noradrenaline probably from adrenergic nerve terminals supplying the entire vasculature, and that acute alterations of circulating DBH activity are not dependent on the rate of catecholamine release evoked by direct electrical stimulation of sympathetic nerves in the whole pithed rat. The rat seems not to be a good model to study circulating noradrenaline and DBH levels as an index of exocytotic noradrenaline release from adrenergic neurones, and therefore of sympathetic activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANTON A. H., SAYRE D. F. A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines. J Pharmacol Exp Ther. 1962 Dec;138:360–375. [PubMed] [Google Scholar]
  2. De Potter W. P., Chubb I. W., Put A., De Schaepdryver A. F. Facilitation of the release of noradrenaline and dopamine- -hydroxylase at low stimulation frequencies by -blocking agents. Arch Int Pharmacodyn Ther. 1971 Sep;193(1):191–197. [PubMed] [Google Scholar]
  3. Dixon W. R., Garcia A. G., Kirpekar S. M. Depletion and recovery of catecholamines in the rat adrenal medulla and its relationship with dopamine beta-hydroxylase. Proc R Soc Lond B Biol Sci. 1976 Oct 29;194(1116):403–416. doi: 10.1098/rspb.1976.0085. [DOI] [PubMed] [Google Scholar]
  4. Dixon W. R., Garcia A. G., Kirpekar S. M. Release of catecholamines and dopamine beta-hydroxylase from the perfused adrenal gland of the cat. J Physiol. 1975 Jan;244(3):805–824. doi: 10.1113/jphysiol.1975.sp010827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GILLESPIE J. S., KIRPEKAR S. M. THE INACTIVATION OF INFUSED NORADRENALINE BY THE CAT SPLEEN. J Physiol. 1965 Jan;176:205–227. doi: 10.1113/jphysiol.1965.sp007545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Garcia A. G., Kirpekar S. M. On the mechanism of release of norepinephrine from cat spleen slices by sodium deprivation and calcium pretreatment. J Pharmacol Exp Ther. 1975 Feb;192(2):343–350. [PubMed] [Google Scholar]
  7. Garcia A. G., Kirpekar S. M., Sanchez-Garcia P. Release of noradrenaline from the cat spleen by nerve stimulation and potassium. J Physiol. 1976 Oct;261(2):301–317. doi: 10.1113/jphysiol.1976.sp011560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gillespie J. S., Maclaren A., Pollock D. A method of stimulating different segments of the autonomic outflow from the spinal column to various organs in the pithed cat and rat. Br J Pharmacol. 1970 Oct;40(2):257–267. doi: 10.1111/j.1476-5381.1970.tb09919.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gillespie J. S., Muir T. C. A method of stimulating the complete sympathetic outflow from the spinal cord to blood vessels in the pithed rat. Br J Pharmacol Chemother. 1967 May;30(1):78–87. doi: 10.1111/j.1476-5381.1967.tb02114.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnson D. G., Thoa N. B., Weinshilboum R., Axelrod J., Kopin I. J. Enhanced release of dopamine- -hydroxylase from sympathetic nerves by calcium and phenoxybenzamine and its reversal by prostaglandins. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2227–2230. doi: 10.1073/pnas.68.9.2227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KIRSHNER N. Uptake of catecholamines by a particulate fraction of the adrenal medulla. J Biol Chem. 1962 Jul;237:2311–2317. [PubMed] [Google Scholar]
  12. Pelayo F. The action of tyramine on the hypertensive responses induced by sympathetic stimulation in the pithed rat. Arch Farmacol Toxicol. 1976 Aug;2(2):105–114. [PubMed] [Google Scholar]
  13. Reid J. L., Kopin I. J. Significance of plasma dopamine beta-hydroxylase activity as an index of sympathetic neuronal function. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4392–4394. doi: 10.1073/pnas.71.11.4392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reid J. L., Kopin I. J. The effects of ganglionic blockade, reserpine and vinblastine on plasma catecholamines and dopamine-beta-hydroxylase in the rat. J Pharmacol Exp Ther. 1975 Jun;193(3):748–756. [PubMed] [Google Scholar]
  15. Schanberg S. M., Kirshner N. Serum dopamine-beta-hydroxylase as an indicator of sympathetic activity and primary hypertension. Biochem Pharmacol. 1976 Mar 15;25(6):617–621. doi: 10.1016/0006-2952(76)90233-1. [DOI] [PubMed] [Google Scholar]
  16. Shellenberger M. K., Gordon J. H. A rapid, simplified procedure for simultaneous assay of norepinephrine, dopamine, and 5-hydroxytryptamine from discrete brain areas. Anal Biochem. 1971 Feb;39(2):356–372. doi: 10.1016/0003-2697(71)90426-x. [DOI] [PubMed] [Google Scholar]
  17. Weinshilboum R. M., Kvetnansky R., Axelrod J., Kopin I. J. Elevation of serum dopamine-beta-hydroxylase activity with forced immobilization. Nat New Biol. 1971 Apr 28;230(17):287–288. doi: 10.1038/newbio230287a0. [DOI] [PubMed] [Google Scholar]
  18. Weinshilboum R. M., Thoa N. B., Johnson D. G., Kopin I. J., Axelrod J. Proportional release of norepinephrine and dopamine- -hydroxylase from sympathetic nerves. Science. 1971 Dec 24;174(4016):1349–1351. doi: 10.1126/science.174.4016.1349. [DOI] [PubMed] [Google Scholar]
  19. Weinshilboum R., Axelrod J. Serum dopamine- -hydroxylase: decrease after chemical sympathectomy. Science. 1971 Sep 3;173(4000):931–934. doi: 10.1126/science.173.4000.931. [DOI] [PubMed] [Google Scholar]

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