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. 1978 Jan;274:265–278. doi: 10.1113/jphysiol.1978.sp012146

Release of endogenous serotonin from two identified serotonin-containing neurones and the physiological role of serotonin re-uptake

M Hamon *, H M Gerschenfeld *, Daniele Paupardin-Tritsch *
PMCID: PMC1282489  PMID: 203673

Abstract

1. The amounts of endogenous serotonin (5-HT) released into the medium by the cerebro-buccal ganglionic ring of Aplysia californica incubated in artificial sea water (ASW) were measured. The rate of spontaneous 5-HT release varied between 0·4 and 1·2 p-mole per hour, which is less than 1% of the total 5-HT present in this preparation.

2. Direct stimulation of the ordinarily silent 5-HT-containing giant cerebral neurones resulted in a 80-100% increase of the 5-HT released, but only when the 5-HT uptake was blocked by chlorimipramine (1-10 μM).

3. High K+ media (50 mM) also caused a significant increase in the amount of 5-HT released from the preparation provided that chlorimipramine (1-10 μM) was present in the incubation fluid.

4. Co2+ ions (10-30 mM) added to the incubating medium blocked the spontaneous leak of endogenous 5-HT as well as the release, in the presence of chlorimipramine, evoked either by stimulation of the 5-HT-giant cerebral neurones or high K+-media.

5. In the presence of chlorimipramine or desmethylimipramine, the duration and/or the amplitude of the excitatory or the inhibitory synaptic potentials evoked in the buccal neurones by the stimulation of the 5-HT giant cerebral neurones were markedly enhanced.

6. These results strongly support the idea that 5-HT is the synaptic transmitter released at the excitatory and inhibitory junctions established by the 5-HT giant cerebral neurones in the ipsilateral buccal ganglia. In addition, they underline the role of amine re-uptake in the physiological inactivation of 5-HT as a transmitter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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