Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1978 Jun;279:141–151. doi: 10.1113/jphysiol.1978.sp012336

Control of calcium channels by membrane receptors in the rat parotid gland.

S H Marier, J W Putney Jr, C M Van de Walle
PMCID: PMC1282607  PMID: 209174

Abstract

1. The mechanism of action of agonists that stimulate K release from the parotid gland was investigated by monitoring efflux of 86Rb from rat parotid slices. 2. As in previous studies, the 86Rb release was increased by agonists in a biphasic manner. An early, transient phase occurred that was independent of extracellular Ca. This was followed by a sustained (or slowly falling phase) that required extracellular Ca. 3. The agonists were investigated as to their additivity and to their sensitivity to inhibition by a number of putative Ca-antagonists. 4. When carbachol, epinephrine and Substance P were employed in supramaximal concentrations, no combination of agonists produced a summated 86Rb release response, despite the fact that the Ca concentration was submaximal. 5. The sustained phase of 86Rb release, which is dependent on extracellular Ca, was blocked by La, Ni, Co and neomycin; the transient phase was unaffected by these agents. 6. The local anaesthetics tetracaine and procaine inhibited both the transient and sustained phases of the responses to carbachol and phenylephrine; responses to Substance P and to the divalent cationophore, A23187, were largely refractory to this effect. 7. These results support the contention that, in the parotid, muscarinic, alpha-adrenergic and peptide receptors regulate the same Ca influx sites. 8. Also, these results suggest that La, Ni, Co and neomycin appear to antagonize the action of Ca by impeding inward movement of the cation through activated Ca influx sites. 9. Finally, the local anesthetics appear to inhibit, and therefore, serve to define, a transduction step between receptor occupation and channel activation. 10. In the case of the Substance P mechanism, it appears that the transduction mechanism must be qualitatively different to that for the muscarinic or alpha-adrenergic mechanisms.

Full text

PDF
141

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURGEN A. S. The secretion of potassium in saliva. J Physiol. 1956 Apr 27;132(1):20–39. doi: 10.1113/jphysiol.1956.sp005500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berridge M. J. The interaction of cyclic nucleotides and calcium in the control of cellular activity. Adv Cyclic Nucleotide Res. 1975;6:1–98. [PubMed] [Google Scholar]
  3. Feinstein M. B. Inhibition of contraction and calcium exchange-ability in rat uterus by local anesthetics. J Pharmacol Exp Ther. 1966 Jun;152(3):516–524. [PubMed] [Google Scholar]
  4. Goodman F. R., Weiss G. B., Adams H. R. Alterations by neomycin of 45Ca movements and contractile responses in vascular smooth muscle. J Pharmacol Exp Ther. 1974 Feb;188(2):472–480. [PubMed] [Google Scholar]
  5. Pedersen G. L., Petersen O. H. Membrane potential measurement in parotid acinar cells. J Physiol. 1973 Oct;234(1):217–227. doi: 10.1113/jphysiol.1973.sp010342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Perkins J. P. Adenyl cyclase. Adv Cyclic Nucleotide Res. 1973;3:1–64. [PubMed] [Google Scholar]
  7. Petersen O. H., Pedersen G. L. Membrane effects mediated by alpha-and beta-adrenoceptors in mouse parotid acinar cells. J Membr Biol. 1974;16(4):353–362. doi: 10.1007/BF01872423. [DOI] [PubMed] [Google Scholar]
  8. Petersen O. H. Some factors influencing stimulation-induced release of potassium from the cat submandibular gland to fluid perfused through the gland. J Physiol. 1970 Jun;208(2):431–447. doi: 10.1113/jphysiol.1970.sp009129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Putney J. W., Jr Biphasic modulation of potassium release in rat parotid gland by carbachol and phenylephrine. J Pharmacol Exp Ther. 1976 Aug;198(2):375–384. [PubMed] [Google Scholar]
  10. Putney J. W., Jr Muscarinic, alpha-adrenergic and peptide receptors regulate the same calcium influx sites in the parotid gland. J Physiol. 1977 Jun;268(1):139–149. doi: 10.1113/jphysiol.1977.sp011851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Putney J. W., Jr Stimulation of 45Ca influx in rat parotid gland by carbachol. J Pharmacol Exp Ther. 1976 Dec;199(3):526–537. [PubMed] [Google Scholar]
  12. Rasmussen H., Goodman D. B. Relationships between calcium and cyclic nucleotides in cell activation. Physiol Rev. 1977 Jul;57(3):421–509. doi: 10.1152/physrev.1977.57.3.421. [DOI] [PubMed] [Google Scholar]
  13. Rudich L., Butcher F. R. Effect of substance P and eledoisin on K+ efflux, amylase release and cyclic nucleotide levels in slices of rat parotid gland. Biochim Biophys Acta. 1976 Oct 22;444(3):704–711. doi: 10.1016/0304-4165(76)90317-2. [DOI] [PubMed] [Google Scholar]
  14. Schramm M., Selinger Z. The function of alpha- and beta-adrenergic receptors and a cholinergic receptor in the secretory cell of rat parotid gland. Adv Cytopharmacol. 1974;2:29–32. [PubMed] [Google Scholar]
  15. Selinger Z., Batzri S., Eimerl S., Schramm M. Calcium and energy requirements for K + release mediated by the epinephrine -receptor in rat parotid slices. J Biol Chem. 1973 Jan 10;248(1):369–372. [PubMed] [Google Scholar]
  16. Selinger Z., Eimerl S., Schramm M. A calcium ionophore simulating the action of epinephrine on the alpha-adrenergic receptor. Proc Natl Acad Sci U S A. 1974 Jan;71(1):128–131. doi: 10.1073/pnas.71.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES