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. 1978 Oct;283:409–424. doi: 10.1113/jphysiol.1978.sp012509

Regulation of calcium fluxes in pancreatic islets: dissociation between calcium and insulin release.

A Herchuelz, W J Malaisse
PMCID: PMC1282786  PMID: 364009

Abstract

1. The release of 45calcium from prelabelled pancreatic islets is rapidly and almost totally inhibited by lanthanum. 2. Glucose provokes an intitial fall followed by a secondary rise in 45calcium efflux. The latter rise occurs concomitantly with insulin release. Its magnitude is reduced whenever the secretory response to glucose is inhibited, e.g. in the absence of extracellular calcium, presence of Verapamil, or at high magnesium concentration. 3. However, under suitable conditions, the glucose-induced secondary rise in 45calcium efflux is not totally suppressed whilst insulin release is totally abolished. 4. Inversely, when calcium is replaced by barium in the perifusate, glucose increases insulin output without causing any obvious secondary rise in 45calcium efflux. 5. It is concluded that this secondary rise, which originates from a lanthanum-nondisplaceable calcium pool, does not correspond solely to an exocytotic release of 45calcium. It could represent, in part at least, a displacement of 45calcium from cellular sites and reflect a glucose-induced increase in the rate of calcium entry in islet cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bukowiecki L., Freinkel N. Relationship between efflux of ionic calcium and phosphorus during excitation of pancreatic islets with glucose. Biochim Biophys Acta. 1976 Jun 4;436(1):190–198. doi: 10.1016/0005-2736(76)90230-3. [DOI] [PubMed] [Google Scholar]
  2. Hales C. N., Milner R. D. Cations and the secretion of insulin from rabbit pancreas in vitro. J Physiol. 1968 Nov;199(1):177–187. doi: 10.1113/jphysiol.1968.sp008647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hellman B., Sehlin J., Täljedal I. B. Effects of glucose on 45Ca2+ uptake by pancreatic islets as studied with the lanthanum method. J Physiol. 1976 Jan;254(3):639–656. doi: 10.1113/jphysiol.1976.sp011250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Herbert V., Lau K. S., Gottlieb C. W., Bleicher S. J. Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab. 1965 Oct;25(10):1375–1384. doi: 10.1210/jcem-25-10-1375. [DOI] [PubMed] [Google Scholar]
  5. Herman L., Sato T., Hales C. N. The electron microscopic localization of cations to pancreatic islets of Langerhans and their possible tole in insulin secretion. J Ultrastruct Res. 1973 Feb;42(3):298–311. doi: 10.1016/s0022-5320(73)90058-0. [DOI] [PubMed] [Google Scholar]
  6. Howell S. L., Tyhurst M. Barium accumulation in rat pancreatic B cells. J Cell Sci. 1976 Nov;22(2):455–465. doi: 10.1242/jcs.22.2.455. [DOI] [PubMed] [Google Scholar]
  7. Malaisse-Lagae F., Malaisse W. J. The stimulus-secretion coupling of glucose-induced insulin release. 3. Uptake of 45 calcium by isolated islets of Langerhans. Endocrinology. 1971 Jan;88(1):72–80. doi: 10.1210/endo-88-1-72. [DOI] [PubMed] [Google Scholar]
  8. Malaisse W. J., Brisson G. R., Baird L. E. Stimulus-secretion coupling of glucose-induced insulin release. X. Effect of glucose on 45 Ca efflux from perifused islets. Am J Physiol. 1973 Feb;224(2):389–394. doi: 10.1152/ajplegacy.1973.224.2.389. [DOI] [PubMed] [Google Scholar]
  9. Malaisse W. J., Brisson G., Malaisse-Lagae F. The stimulus-secretion coupling of glucose-induced insulin release. I. Interaction of epinephrine and alkaline earth cations. J Lab Clin Med. 1970 Dec;76(6):895–902. [PubMed] [Google Scholar]
  10. Malaisse W. J., Herchuelz A., Devis G., Somers G., Boschero A. C., Hutton J. C., Kawazu S., Sener A., Atwater I. J., Duncan G. Regulation of calcium fluxes and their regulatory roles in pancreatic islets. Ann N Y Acad Sci. 1978 Apr 28;307:562–582. doi: 10.1111/j.1749-6632.1978.tb41982.x. [DOI] [PubMed] [Google Scholar]
  11. Malaisse W. J., Herchuelz A., Levy J., Sener A. Calcium antagonists and islet function--III. The possible site of action of verapamil. Biochem Pharmacol. 1977 Apr 15;26(8):735–740. doi: 10.1016/0006-2952(77)90217-9. [DOI] [PubMed] [Google Scholar]
  12. Malaisse W. J., Hutton J. C., Sener A., Levy J., Herchuelz A., Devis G., Somers G. Calcium antagonists and islet function: VII. Effect of calcium deprivation. J Membr Biol. 1978 Jan 18;38(3):193–208. doi: 10.1007/BF01871922. [DOI] [PubMed] [Google Scholar]
  13. Malaisse W. J. Insulin secretion: multifactorial regulation for a single process of release. The Minkowski award lecture delivered on September 7, 1972 before the European Association for the study of Diabetes at Madrid, Spain. Diabetologia. 1973 Jun;9(3):167–173. doi: 10.1007/BF01219778. [DOI] [PubMed] [Google Scholar]
  14. Milner R. D., Hales C. N. The role of calcium and magnesium in insulin secretion from rabbit pancreas studied in vitro. Diabetologia. 1967 Mar;3(1):47–49. doi: 10.1007/BF01269910. [DOI] [PubMed] [Google Scholar]
  15. Naber S. P., McDaniel M. L., Lacy P. E. The effect of glucose on the acute uptake and efflux of calcium-45 in isolated rat islets. Endocrinology. 1977 Sep;101(3):686–693. doi: 10.1210/endo-101-3-686. [DOI] [PubMed] [Google Scholar]
  16. Ravazzola M., Malaisse-Lagae F., Amherdt M., Perrelet A., Malaisse W. J., Orci L. Patterns of calcium localization in pancreatic endocrine cells. J Cell Sci. 1976 Jun;21(1):107–117. doi: 10.1242/jcs.21.1.107. [DOI] [PubMed] [Google Scholar]
  17. Schäfer H. J., Klöppel G. The significance of calcium in insulin secretion. Ultrastructural studies on identification and localization of calcium in activated and inactivated B cells of mice. Virchows Arch A Pathol Anat Histol. 1974;362(3):231–245. doi: 10.1007/BF00432197. [DOI] [PubMed] [Google Scholar]
  18. Somers G., Devis G., van Obberghen E., Malaisse W. J. Calcium-antagonists and islet function. VI. Effects of barium. Pflugers Arch. 1976 Sep 3;365(1):21–28. doi: 10.1007/BF00583624. [DOI] [PubMed] [Google Scholar]
  19. Wollheim C. B., Blondel B., Trueheart P. A., Renold A. E., Sharp G. W. Calcium-induced insulin release in monolayer culture of the endocrine pancreas. Studies with ionophore A23187. J Biol Chem. 1975 Feb 25;250(4):1354–1360. [PubMed] [Google Scholar]

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