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. 1978 Nov;284:203–217. doi: 10.1113/jphysiol.1978.sp012536

The effect of increasing the plasma magnesium concentration on renin release from the dog's kidney: interactions with calcium and sodium.

C S Wilcox
PMCID: PMC1282817  PMID: 366108

Abstract

1. A denervated 'auto-transplanted' dog's kidney preparation was developed to study renin release into renal plasma and lymph. The function of the 'transplant' was compared with that of its partner. In the 'basal' state it had a similar rate of plasma and urine flow, Na, Ca, Mg and Cl excretion but a lower rate of glomerular filtration and K excretion and a lower urinary osmolality. In the 'basal' state the 'transplant' did not release renin into plasma, but invariably released it into lymph. 2. Infusions of MgCl2 solutions into the renal artery which raised the renal plasma Mg concentration (PMg) by 0.1-2 m-mole.1.-1 provoked a concentration-related increase in renin release into plasma. This was due to a rise in the veno-arterial renin difference and in the renal plasma flow rate. Blood pressure and Na excretion were unaltered. 3. In other experiments, an increase in PMg of 1.5-2.5 m-mole.1.-1 was also found to increase renin release into lymph. 4. When the plasma Ca concentration was doubled by infusion of CaCl2 into one renal artery, an increase in PMg of 1.5-2.5 m-mole.1.-1 no longer increased renin release into plasma or lymph. 5. When the plasma NaCl concentration was raised by 8-15 m-mole.1.-1 by infusion of hypertonic saline into the renal artery, MgCl2 infusion failed to increase renin release until PMg was raised by more than 3 m-mole.1-1. 6. The results demonstrate that hypermagnesaemia stimulates renal renin release by a mechanism that is independent of the renal nerves, or of any changes in blood pressure or sodium excretion, but which is antagonized by concurrent hypercalcaemia or hypersalaemia. The possibility is discussed that Mg is reabsorbed from the tubular into the interstitial fluid where it antagonizes the action(s) of Ca on renin release from the juxtaglomerular cells.

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Selected References

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  1. Altura B. M., Altura B. T. Differential effects of substrate depletion on drug-induced contractions of rabbit aorta. Am J Physiol. 1970 Dec;219(6):1698–1705. doi: 10.1152/ajplegacy.1970.219.6.1698. [DOI] [PubMed] [Google Scholar]
  2. Altura B. M., Altura B. T. Magnesium and contraction of arterial smooth muscle. Microvasc Res. 1974 Mar;7(2):145–155. doi: 10.1016/0026-2862(74)90001-6. [DOI] [PubMed] [Google Scholar]
  3. Boyd G. W., Fitz A. E., Adamson A. R., Peart W. S. Radioimmunoassay determination of plasma-renin activity. Lancet. 1969 Feb 1;1(7588):213–218. doi: 10.1016/s0140-6736(69)91237-9. [DOI] [PubMed] [Google Scholar]
  4. Brubacher E. S., Vander A. J. Sodium deprivation and renin secretion in unanesthetized dogs. Am J Physiol. 1968 Jan;214(1):15–21. doi: 10.1152/ajplegacy.1968.214.1.15. [DOI] [PubMed] [Google Scholar]
  5. Churchill P. C., Lyons H. J. Effect of intrarenal arterial infusion of magnesium on renin release in dogs. Proc Soc Exp Biol Med. 1976 May;152(1):6–10. doi: 10.3181/00379727-152-39315. [DOI] [PubMed] [Google Scholar]
  6. Coote J. H., Johns E. J., Macleod V. H., Singer B. Effect of renal nerve stimulation, renal blood flow and adrenergic blockade on plasma renin activity in the cat. J Physiol. 1972 Oct;226(1):15–36. doi: 10.1113/jphysiol.1972.sp009971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eide I., Loyning E., Kiil F. Evidence for hemodynamic autoregulation of renin release. Circ Res. 1973 Feb;32(2):237–245. doi: 10.1161/01.res.32.2.237. [DOI] [PubMed] [Google Scholar]
  8. Fray J. S. Stimulation of renin release in perfused kidney by low calcium and high magnesium. Am J Physiol. 1977 Apr;232(4):F377–F382. doi: 10.1152/ajprenal.1977.232.4.F377. [DOI] [PubMed] [Google Scholar]
  9. Fynn M., Onomakpome N., Peart W. S. The effects of ionophores (A23187 and RO2-2985) on renin secretion and renal vasoconstriction. Proc R Soc Lond B Biol Sci. 1977 Nov 14;199(1135):199–212. doi: 10.1098/rspb.1977.0135. [DOI] [PubMed] [Google Scholar]
  10. Iwao H., Abe Y., Yamamoto K. Effect of intrarenal arterial infusion of calcium on renin release in dogs. Jpn J Pharmacol. 1974 Jun;24(3):482–484. doi: 10.1254/jjp.24.482. [DOI] [PubMed] [Google Scholar]
  11. Kotchen T. A., Galla J. H., Luke R. G. Effects of calcium on renin and aldosterone in the rat. Am J Physiol. 1977 Apr;232(4):E388–E393. doi: 10.1152/ajpendo.1977.232.4.E388. [DOI] [PubMed] [Google Scholar]
  12. Kotchen T. A., Mauli K. I., Luke R., Rees D., Flamenbaum W. Effect of acute and chronic calcium administration on plasma renin. J Clin Invest. 1974 Dec;54(6):1279–1286. doi: 10.1172/JCI107873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LEVER A. F., PEART W. S. Renin and angiotensin-like activity in renal lymph. J Physiol. 1962 Mar;160:548–563. doi: 10.1113/jphysiol.1962.sp006865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lester G. E., Rubin R. P. The role of calcium in renin secretion from the isolated perfused cat kidney. J Physiol. 1977 Jul;269(1):93–108. doi: 10.1113/jphysiol.1977.sp011894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lichardus B., Nizet A. Water and sodium excretion after blood volume expansion under conditions of constant arterial, venous and plasma oncotic pressures and constant haematocrit. Clin Sci. 1972 Jun;42(6):701–709. doi: 10.1042/cs0420701. [DOI] [PubMed] [Google Scholar]
  16. Logan A. G., Tenyi I., Peart W. S., Breathnach A. S., Martin B. G. The effect of lanthanum on renin secretion and renal vasoconstriction. Proc R Soc Lond B Biol Sci. 1977 Jan 14;195(1120):327–342. doi: 10.1098/rspb.1977.0013. [DOI] [PubMed] [Google Scholar]
  17. Morgan T., Gillies A. Factors controlling the release of renin. A micropuncture study in the cat. Pflugers Arch. 1977 Mar 11;368(1-2):13–18. doi: 10.1007/BF01063449. [DOI] [PubMed] [Google Scholar]
  18. Nashat F. S., Tappin J. W., Wilcox C. S. Plasma sodium concentration and sodium excretion in the anaesthetized dog. J Physiol. 1976 Jan;254(1):183–202. doi: 10.1113/jphysiol.1976.sp011228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Peart W. S. The kidney as an endocrine organ. Lancet. 1977 Sep 10;2(8037):543–548. [PubMed] [Google Scholar]
  20. Reinhardt H. W., Klose H. J., Ellinghaus K., Brechtelsbauer H., Behrenbeck D. W. Ein Nierenpräparat (sog. Halsniere) zur Erleichterung komplizierter Funktionsuntersuchungen. Homotransplantat am narkotisierten Hund. Pflugers Arch Gesamte Physiol Menschen Tiere. 1967;297(2):71–88. [PubMed] [Google Scholar]
  21. Rubin R. P. The role of calcium in the release of neurotransmitter substances and hormones. Pharmacol Rev. 1970 Sep;22(3):389–428. [PubMed] [Google Scholar]
  22. Shade R. E., Davis J. O., Johnson J. A., Witty R. T. Effects of renal arterial infusion of sodium and potassium on renin secretion in the dog. Circ Res. 1972 Nov;31(5):719–727. doi: 10.1161/01.res.31.5.719. [DOI] [PubMed] [Google Scholar]
  23. Skinner S. L. Improved assay methods for renin "concentration" and "activity" in human plasma. Methods using selective denaturation of renin substrate. Circ Res. 1967 Apr;20(4):391–402. doi: 10.1161/01.res.20.4.391. [DOI] [PubMed] [Google Scholar]
  24. Tobian L. Renin release and its role in renal function and the control of salt balance and arterial pressure. Fed Proc. 1967 Jan-Feb;26(1):48–54. [PubMed] [Google Scholar]
  25. VANDER A. J., MILLER R. CONTROL OF RENIN SECRETION IN THE ANESTHETIZED DOG. Am J Physiol. 1964 Sep;207:537–546. doi: 10.1152/ajplegacy.1964.207.3.537. [DOI] [PubMed] [Google Scholar]
  26. Van Dongen R., Peart W. S. Calcium dependence of the inhibitory effect of angiotensin on renin secretion in the isolated perfused kidney of the rat. Br J Pharmacol. 1974 Jan;50(1):125–129. doi: 10.1111/j.1476-5381.1974.tb09599.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Vander A. J. Control of renin release. Physiol Rev. 1967 Jul;47(3):359–382. doi: 10.1152/physrev.1967.47.3.359. [DOI] [PubMed] [Google Scholar]
  28. Warren R. L., Wilcox C. S. The excretion of magnesium and calcium by the dog kidney during an acute increase in the plasma sodium chloride concentration [proceedings]. J Physiol. 1977 Jun;268(1):12P–13P. [PubMed] [Google Scholar]
  29. Watkins B. E., Davis J. O., Lohmeier T. E., Freeman R. H. Intrarenal site of action of calcium on renin secretion in dogs. Circ Res. 1976 Dec;39(6):847–853. doi: 10.1161/01.res.39.6.847. [DOI] [PubMed] [Google Scholar]
  30. Wilcox C. S. Renin release from the dog's kidney during hypermagnesaemia. J Physiol. 1978 Feb;275:25P–26P. [PubMed] [Google Scholar]

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