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. 1980 May;302:89–105. doi: 10.1113/jphysiol.1980.sp013231

Alpha-aminoisobutyric acid transport in rat soleus muscle and its modification by membrane stabilizers and insulin.

G J Cooper, P G Kohn
PMCID: PMC1282836  PMID: 6997458

Abstract

The non-metabolizable amino acid alpha-aminoisobutyric acid (AIB) has been used to study the effects of insulin and a number of membrane stabilizers on amino acid transport in rat soleus muscle in an attempt to characterize the mechanisms present. 2. Insulin (5--100 millimicron./ml) increases the net uptake of AIB two- to threefold. Since insulin is without significant effects on AIB efflux, stimulation of net uptake appears to result directly from an increased AIB influx. 3. All classes of membrane stabilizer tested affected AIB fluxes but the responses observed varied for different classes of compounds. 4. The total anaesthetic, tetracaine, reduced AIB accumulation both in the absence and presence of insulin by a similar proportion. The effects on AIB efflux were dependent on the concentration of tetracaine used. Efflux was suppressed by concentrations up to 1 mM whereas 4 mM-tetracaine caused a massive stimulation of AIB efflux. Other local anaesthetics and barbiturates produced similar effects. 5. Another group of membrane stabilizers, exemplified by chlorpromazine, also suppressed AIB uptake, but at no concentration did they reduce AIB efflux. In fact, efflux of AIB began to be increased at concentrations of chlorpromazine which were giving only a modest inhibition of uptake. 6. Measurement of the initial rate of uptake showed that it involved two components. Tetracaine appears to inhibit the saturable component whilst leaving the non-saturable component relatively unaffected. 7. The active uptake of AIB was shown to be Na+-dependent, and under Na+-free conditions tetracaine had no effect on the initial rate of uptake. The non-saturable component was also shown to be Na+-sensitive, uptake from high extracellular concentrations of AIB being reduced in Na+-free media. 8. The possibility of the presence of a carrier-mediated AIB efflux mechanism was investigated. AIB efflux was stimulated by extracellular AIB (homo-exchange) or glycine (hetero-exchange), but not mannitol. This suggests the involvement of a carrier-mediated process in AIB efflux. 9. The present study has demonstrated a heterogeneity among different classes of membrane stabilizers in their actions on AIB efflux which is in marked contrast to previous observations of sugar and cation transport in this preparation. Possible reasons for these differences are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AKEDO H., CHRISTENSEN H. N. Nature of insulin action on amino acid uptake by the isolated diaphragm. J Biol Chem. 1962 Jan;237:118–122. [PubMed] [Google Scholar]
  2. AKEDO H., CHRISTENSEN H. N. Transfer of amino acids across the intestine: a new model amino acid. J Biol Chem. 1962 Jan;237:113–117. [PubMed] [Google Scholar]
  3. Andersen N. B., Amaranath L. Anesthetic effects on transport across cell membranes. Anesthesiology. 1973 Aug;39(2):126–152. doi: 10.1097/00000542-197308000-00005. [DOI] [PubMed] [Google Scholar]
  4. CHRISTENSEN H. N., ASPEN A. J., RICE E. G. Metabolism in the rat of three amino acids lacking alpha-hydrogen. J Biol Chem. 1956 May;220(1):287–294. [PubMed] [Google Scholar]
  5. CHRISTENSEN H. N., PARKER H. M., RIGGS T. R. Non-exchange of carboxyl oxygen in mammalian amino acid transport. J Biol Chem. 1958 Dec;233(6):1485–1487. [PubMed] [Google Scholar]
  6. CHRISTENSEN H. N., RIGGS T. R. Structural evidences for chelation and Schiff's base formation in amino acid transfer into cells. J Biol Chem. 1956 May;220(1):265–278. [PubMed] [Google Scholar]
  7. Chaudry I. H., Gould M. K. Kinetics of glucose uptake in isolated soleus muscle. Biochim Biophys Acta. 1969 May 6;177(3):527–536. doi: 10.1016/0304-4165(69)90315-8. [DOI] [PubMed] [Google Scholar]
  8. Chinet A., Clausen T., Girardier L. Microcalorimetric determination of energy expenditure due to active sodium-potassium transport in the soleus muscle and brown adipose tissue of the rat. J Physiol. 1977 Feb;265(1):43–61. doi: 10.1113/jphysiol.1977.sp011704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Clausen T., Harving H., Dahl-Hansen A. B. The relationship between the transport of glucose and cations across cell membranes in isolated tissues. 8. The effect of membrane stabilizers on the transport of K + , Na + and glucose in muscle, adipocytes and erythrocytes. Biochim Biophys Acta. 1973 Mar 16;298(2):393–411. doi: 10.1016/0005-2736(73)90367-2. [DOI] [PubMed] [Google Scholar]
  10. Clausen T., Kohn P. G. The effect of insulin on the transport of sodium and potassium in rat soleus muscle. J Physiol. 1977 Feb;265(1):19–42. doi: 10.1113/jphysiol.1977.sp011703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Clausen T. The relationship between the transport of glucose and cations across cell membranes in isolated tissues. V. Stimulating effect of ouabain, K+-free medium and insulin on efflux of 3-O-methylglucose from epidimal adipose tissue. Biochim Biophys Acta. 1969;183(3):625–634. doi: 10.1016/0005-2736(69)90175-8. [DOI] [PubMed] [Google Scholar]
  12. Cooper G. J., Kohn P. G. The effect of tetracaine on 2-amino-iso-butyric acid transport in muscle. J Physiol. 1973 Feb;229(1):23P–24P. [PubMed] [Google Scholar]
  13. Cooper G. J., Kohn P. G. The mechanism of 2-amino-iso-butyric acid efflux from rat soleus muscle and its modification by the membrane stabilizer, tetracaine [proceedings]. J Physiol. 1977 Oct;271(2):20P–20P. [PubMed] [Google Scholar]
  14. Dahl-Hansen A. B., Clausen T. The effect of membrane stabilizers and ouabain on the transport of Na+ and K+ in rat soleus muscle. Biochim Biophys Acta. 1973 Aug 9;318(1):147–153. doi: 10.1016/0005-2736(73)90344-1. [DOI] [PubMed] [Google Scholar]
  15. Guidotti G. G., Borghetti A. F., Gaja G., Loreti L., Ragnotti G., Foà P. P. Amino acid uptake in the developing chick embryo heart. The effect of insulin on alpha-aminoisobutyric acid accumulation. Biochem J. 1968 Apr;107(4):565–574. doi: 10.1042/bj1070565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. KIPNIS D. M., NOALL M. W. Stimulation of amino acid transport by insulin in the isolated rat diaphragm. Biochim Biophys Acta. 1958 Apr;28(1):226–227. doi: 10.1016/0006-3002(58)90466-9. [DOI] [PubMed] [Google Scholar]
  17. Kohn P. G., Clausen T. The relationship between the transport of glucose and cations across cell membranes in isolated tissues. VII. The effects of extracellular Na + and K + on the transport of 3-O-methylglucose and glucose in rat soleus muscle. Biochim Biophys Acta. 1972 Mar 17;255(3):798–814. doi: 10.1016/0005-2736(72)90392-6. [DOI] [PubMed] [Google Scholar]
  18. Manchester K. L., Guidotti G. G., Borghetti A. F., Lüneburg B. Evaluation of kinetic parameters for uptake of amino acids by cells: effect of insulin on the accumulation of aminoisobutyrate and cycloleucine by isolated rat diaphragm muscle and chick embryo hearts. Biochim Biophys Acta. 1971 Jul 6;241(1):226–241. doi: 10.1016/0005-2736(71)90319-1. [DOI] [PubMed] [Google Scholar]
  19. Riggs T. R., McKirahan K. J. Action of insulin on transport of L-alanine into rat diaphragm in vitro. Evidence that the hormone affects only one neutral amino acid transport system. J Biol Chem. 1973 Sep 25;248(18):6450–6455. [PubMed] [Google Scholar]
  20. SHANES A. M. Electrochemical aspects of physiological and pharmacological action in excitable cells. II. The action potential and excitation. Pharmacol Rev. 1958 Jun;10(2):165–273. [PubMed] [Google Scholar]
  21. Seeman P. The membrane actions of anesthetics and tranquilizers. Pharmacol Rev. 1972 Dec;24(4):583–655. [PubMed] [Google Scholar]

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