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. 1980 Aug;305:315–325. doi: 10.1113/jphysiol.1980.sp013365

Effects of sugars, amino acids and inhibitors on electrolyte transport across hen colon at different sodium chloride intakes.

J Lind, B G Munck, O Olsen, E Skadhauge
PMCID: PMC1282974  PMID: 7441557

Abstract

1. The isolated colonic mucosa from adult hens has been studied in vitro under short-circuit conditions. Colonic mucosa was prepared from hens fed either a NaCl-poor or a NaCl-rich diet. 2. The unidirectional transmucosal fluxes of chloride, K and Na were measured. For the high NaCl colon the fluxes of lysine were also measured. The effects of galactose, glucose, leucine, lysine, amiloride (10(-4) M) and acetazolamide (5 X 10(-4) M) on the short-circuit current (Isc) were examined. 3. The Isc of the high NaCl colon was stimulated by galactose, glucose, leucine and lysine in a manner which suggests that these substances were transported by the epithelium in co-transport with Na. The Isc was in most cases insensitive to amiloride (mucosal side), but moderately stimulated by acetazolamide (serosal side). The Isc was closely matched by the sum of the net transmucosal fluxes of chloride, lysine, K and Na. The effects of galactose, leucine and lysine on the Isc were maintained only when glucose was present on the serosal side of the preparations. 4. The Isc of the low NaCl colon depended on the presence of glucose on the serosal (or mucosal) side. It was not stimulated by galactose or by lysine, but leucine did stimulate when added to the mucosal solution. Adding amiloride on the mucosal side invariably eliminated or, in most cases, led to an inversion of the Isc. Acetazolamide markedly stimulated the Isc; and, when added after amiloride, acetazolamide caused the Isc to become positive again. The data on Isc and net fluxes of chloride, lysine, K and Na are consistent with the presence of a process of bicarbonate absorption or H ion secretion. 5. There is a net secretion of K across both the high and the low Na colon, which is highest in the former although both Isc and net absorption of Na are highest in the latter. 6. The results from both types of colon suggest that the transport of chloride differs with the functional status of tissues.

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Selected References

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  1. Al-Awqati Q. H + transport in urinary epithelia. Am J Physiol. 1978 Aug;235(2):F77–F88. doi: 10.1152/ajprenal.1978.235.2.F77. [DOI] [PubMed] [Google Scholar]
  2. Al-Awqati Q., Norby L. H., Mueller A., Steinmetz P. R. Characteristics of stimulation of H+ transport by aldosterone in turtle urinary bladder. J Clin Invest. 1976 Aug;58(2):351–358. doi: 10.1172/JCI108479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bastl C., Kliger A. S., Binder H. J., Hayslett J. P. Characteristics of potassium secretion in the mammalian colon. Am J Physiol. 1978 Jan;234(1):F48–F53. doi: 10.1152/ajprenal.1978.234.1.F48. [DOI] [PubMed] [Google Scholar]
  4. Binder H. J., Rawlins C. L. Electrolyte transport across isolated large intestinal mucosa. Am J Physiol. 1973 Nov;225(5):1232–1239. doi: 10.1152/ajplegacy.1973.225.5.1232. [DOI] [PubMed] [Google Scholar]
  5. Brodsky W. A., Durham J., Ehrenspeck G. The effects of a disulphonic stilbene on chloride and bicarbonate transport in the turtle bladder. J Physiol. 1979 Feb;287:559–573. doi: 10.1113/jphysiol.1979.sp012677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Choshniak I., Munck B. G., Skadhauge E. Sodium chloride transport across the chicken coprodeum. Basic characteristics and dependence on sodium chloride intake. J Physiol. 1977 Oct;271(2):489–503. doi: 10.1113/jphysiol.1977.sp012010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cofré G., Crabbé J. Stimulation by aldosterone of active sodium transport by the isolated colon of the toad, Bufo marinus. Nature. 1965 Sep 18;207(5003):1299–1300. doi: 10.1038/2071299a0. [DOI] [PubMed] [Google Scholar]
  8. Edmonds C. J., Marriott J. C. The effect of aldosterone on the electrical activity of rat colon. J Endocrinol. 1969 Jul;44(3):363–377. doi: 10.1677/joe.0.0440363. [DOI] [PubMed] [Google Scholar]
  9. Edmonds C. J. Transport of sodium and secretion of potassium and bicarbonate by the colon of normal and sodium-depleted rats. J Physiol. 1967 Dec;193(3):589–602. doi: 10.1113/jphysiol.1967.sp008380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ehrenspeck G., Durham J., Brodsky W. A. Amiloride-induced stimulation of HCO-3 reabsorption in turtle bladder. Biochim Biophys Acta. 1978 May 18;509(2):390–394. doi: 10.1016/0005-2736(78)90057-3. [DOI] [PubMed] [Google Scholar]
  11. Frizzell R. A., Koch M. J., Schultz S. G. Ion transport by rabbit colon. I. Active and passive components. J Membr Biol. 1976;27(3):297–316. doi: 10.1007/BF01869142. [DOI] [PubMed] [Google Scholar]
  12. Frizzell R. A., Schultz S. G. Effect of aldosterone on ion transport by rabbit colon in vitro. J Membr Biol. 1978 Feb 6;39(1):1–26. doi: 10.1007/BF01872752. [DOI] [PubMed] [Google Scholar]
  13. Hawker P. C., Mashiter K. E., Turnberg L. A. Mechanisms of transport of Na, Cl, and K in the human colon. Gastroenterology. 1978 Jun;74(6):1241–1247. [PubMed] [Google Scholar]
  14. Lind J., Munck B. G., Olsen O. Effects of dietary intake of sodium chloride on sugar and amino acid transport across isolated hen colon. J Physiol. 1980 Aug;305:327–336. doi: 10.1113/jphysiol.1980.sp013366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ludens J. H., Fanestil D. D. Aldosterone stimulation of acidification of urine by isolated urinary bladder of the Colombian toad. Am J Physiol. 1974 Jun;226(6):1321–1326. doi: 10.1152/ajplegacy.1974.226.6.1321. [DOI] [PubMed] [Google Scholar]
  16. Lyngdorf-Henriksen P., Munck B. G., Skadhauge E. Sodium chloride transport across the lower intestine of the chicken. Dependence on sodium chloride concentration and effect of inhibitors. Pflugers Arch. 1978 Dec 28;378(2):161–165. doi: 10.1007/BF00584450. [DOI] [PubMed] [Google Scholar]
  17. Munck B. G., Rasmussen S. N. Paracellular permeability of extracellular space markers across rat jejunum in vitro. Indication of a transepithelial fluid circuit. J Physiol. 1977 Oct;271(2):473–488. doi: 10.1113/jphysiol.1977.sp012009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nellans H. N., Schultz S. G. Relations among transepithelial sodium transport, potassium exchange, and cell volume in rabbit ileum. J Gen Physiol. 1976 Oct;68(4):441–463. doi: 10.1085/jgp.68.4.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schilb T. P., Brodsky W. A. CO 2 gradients and acidification by transport of HCO 3 in turtle bladders. Am J Physiol. 1972 Feb;222(2):272–281. doi: 10.1152/ajplegacy.1972.222.2.272. [DOI] [PubMed] [Google Scholar]
  20. Schultz S. G., Curran P. F. Coupled transport of sodium and organic solutes. Physiol Rev. 1970 Oct;50(4):637–718. doi: 10.1152/physrev.1970.50.4.637. [DOI] [PubMed] [Google Scholar]
  21. Schultz S. G., Frizzell R. A. An overview of intestinal absorptive and secretory processes. Gastroenterology. 1972 Jul;63(1):161–170. [PubMed] [Google Scholar]
  22. Thompson S. M., Dawson D. C. Sodium uptake across the apical border of the isolated turtle colon: confirmation of the two-barrier model. J Membr Biol. 1978 Sep 25;42(4):357–374. doi: 10.1007/BF01870356. [DOI] [PubMed] [Google Scholar]
  23. USSING H. H., ZERAHN K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol Scand. 1951 Aug 25;23(2-3):110–127. doi: 10.1111/j.1748-1716.1951.tb00800.x. [DOI] [PubMed] [Google Scholar]

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