Skip to main content
NCBI home page
The Journal of Physiology logoLink to The Journal of Physiology
. 1977 Apr;266(2):235–254. doi: 10.1113/jphysiol.1977.sp011766

Ouabain-sensitive ion fluxes in the smooth muscle of the guinea-pig's taenia coli.

J H Widdicombe
PMCID: PMC1283564  PMID: 857001

Abstract

1. Tissues with raised intracellular Na levels, produced by incubation in K-free media, were used throughout. The uptake of 42K by these Na-loaded tissues was followed for 10 min in the presence and absence of 1-37 X 10(-4) M ouabain, this being sufficient to inhibit Na pumping maximally. Subtraction of the uptake seen in the presence from that seen in the absence of ouabain gave estimates of the pumped ouabain-sensitive K uptake. 2. In Na-free (MgCl2) medium this depended on the [K]0 in a sigmoidal fashion with a half maximal [K]0 for activation of some 4mM. The maximal uptake of K was 3 m-mole/kg.min corresponding to a transmembrane flux of some 12-5 p-mole. cm-2.sec-1. 3. In the presence of Na the K activation curve became more obviously sigmoid and higher concentrations of K were needed to achieve a given active K influx. The results were well fitted by assuming that Na and K competed for two identical, non-interacting sites on the external pump face. 4. Addition of K during the efflux of 24Na into a Na-free (MgCl2) medium led to an increased rate of tracer loss. The magnitude of this increase depended on the [K] used in a hyperbolic fashion and it was abolished by addition of ouabain. The [K] causing half-maximal activation of ouabain-sensitive Na efflux was in the order of 1-2 mM. 5. When the [K] in the uptake media was 1-5 mM; Na, Li, Rb and Cs all inhibited ouabain-sensitive K uptake, the order of effectiveness being Rb greater than Cs greater than Na greater than Li. With a E1TKA10 OF 0-15 MM low concentrations of Cs and Rb were shown to stimulate K uptake. Such an effect is predicted by assuming two ion binding sites on the pump's outer face, and that the pump can translocate mixtures of K and either Rb or Cs...

Full text

PDF
235

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axelsson J., Holmberg B. The effects of K plus -free solution on tension development in the smooth muscle taenia coli from the guinea pig. Acta Physiol Scand. 1971 Jul;82(3):322–332. doi: 10.1111/j.1748-1716.1971.tb04973.x. [DOI] [PubMed] [Google Scholar]
  2. Baker P. F., Blaustein M. P., Keynes R. D., Manil J., Shaw T. I., Steinhardt R. A. The ouabain-sensitive fluxes of sodium and potassium in squid giant axons. J Physiol. 1969 Feb;200(2):459–496. doi: 10.1113/jphysiol.1969.sp008703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker P. F., Connelly C. M. Some properties of the external activation site of the sodium pump in crab nerve. J Physiol. 1966 Jul;185(2):270–297. doi: 10.1113/jphysiol.1966.sp007987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baker P. F., Willis J. S. Binding of the cardiac glycoside ouabain to intact cells. J Physiol. 1972 Jul;224(2):441–462. doi: 10.1113/jphysiol.1972.sp009904. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bolton T. B. Effects of electrogenic sodium pumping on the membrane potential of longitudinal smooth muscle from terminal ileum of guinea-pig. J Physiol. 1973 Feb;228(3):693–712. doi: 10.1113/jphysiol.1973.sp010107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brading A. F. A constant flow apparatus for measuring radioactive ion effluxes from guinea-pig taenia coli. J Physiol. 1967 Sep;192(2):15P–16P. [PubMed] [Google Scholar]
  7. Brading A. F. Analysis of the effluxes of sodium, potassium and chloride ions from smooth muscle in normal and hypertonic solutions. J Physiol. 1971 May;214(3):393–416. doi: 10.1113/jphysiol.1971.sp009440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brading A. F. Sodium/sodium exchange in the smooth muscle of the guinea-pig taenia coli. J Physiol. 1975 Sep;251(1):79–105. doi: 10.1113/jphysiol.1975.sp011082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Brading A. F., Widdicombe J. H. An estimate of sodium-potassium pump activity and the number of pump sites in the smooth muscle of the guinea-pig taenia coli, using (3H)ouabain. J Physiol. 1974 Apr;238(2):235–249. doi: 10.1113/jphysiol.1974.sp010521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Casteels R., Droogmans G., Hendrickx H. Active ion transport and resting potential in smooth muscle cells. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):47–56. doi: 10.1098/rstb.1973.0008. [DOI] [PubMed] [Google Scholar]
  11. Casteels R., Droogmans G., Hendrickx H. Effect of sodium and sodium-substitutes on the active ion transport and on the membrane potential of smooth muscle cells. J Physiol. 1973 Feb;228(3):733–748. doi: 10.1113/jphysiol.1973.sp010109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Casteels R., Droogmans G., Hendrickx H. Electrogenic sodium pump in smooth muscle cells of the guinea-pig's taenia coli. J Physiol. 1971 Sep;217(2):297–313. doi: 10.1113/jphysiol.1971.sp009572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Casteels R. The action of ouabain on the smooth muscle cells of the guinea-pig's taenia coli. J Physiol. 1966 May;184(1):131–142. doi: 10.1113/jphysiol.1966.sp007907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Cavieres J. D., Ellory J. C. Thallium and the sodium pump in human red cells. J Physiol. 1974 Nov;243(1):243–266. doi: 10.1113/jphysiol.1974.sp010752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. FREEMAN-NARROD M., GOODFORD P. J. Sodium and potassium content of the smooth muscle of the guinea-pig taenia coli at different temperatures and tensions. J Physiol. 1962 Oct;163:399–410. doi: 10.1113/jphysiol.1962.sp006985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. GOODFORD P. J., HERMANSEN K. Sodium and potassium movements in the unstriated muscle of the guinea-pig taenia coli. J Physiol. 1961 Oct;158:426–448. doi: 10.1113/jphysiol.1961.sp006778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Glynn I. M., Karlish S. J. The sodium pump. Annu Rev Physiol. 1975;37:13–55. doi: 10.1146/annurev.ph.37.030175.000305. [DOI] [PubMed] [Google Scholar]
  18. Godfraind T., Lesne M. The uptake of cardiac glycosides by intestinal smooth muscle of the guinea-pig in relation to digitalis receptors. Br J Pharmacol. 1970 Feb;38(2):345–352. doi: 10.1111/j.1476-5381.1970.tb08522.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. HODGKIN A. L., KEYNES R. D. The potassium permeability of a giant nerve fibre. J Physiol. 1955 Apr 28;128(1):61–88. doi: 10.1113/jphysiol.1955.sp005291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Meech R. W. Intracellular calcium injection causes increased potassium conductance in Aplysia nerve cells. Comp Biochem Physiol A Comp Physiol. 1972 Jun 1;42(2):493–499. doi: 10.1016/0300-9629(72)90128-4. [DOI] [PubMed] [Google Scholar]
  21. Rang H. P., Ritchie J. M. On the electrogenic sodium pump in mammalian non-myelinated nerve fibres and its activation by various external cations. J Physiol. 1968 May;196(1):183–221. doi: 10.1113/jphysiol.1968.sp008502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rang H. P., Ritchie J. M. The dependence on external cations of the oxygen consumption of mammalian non-myelinated fibres at rest and during activity. J Physiol. 1968 May;196(1):163–181. doi: 10.1113/jphysiol.1968.sp008501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sachs J. R., Welt L. G. The concentration dependence of active potassium transport in the human red blood cell. J Clin Invest. 1967 Jan;46(1):65–76. doi: 10.1172/JCI105512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sjodin R. A., Beaugé L. A. Coupling and selectivity of sodium and potassium transport in squid giant axons. J Gen Physiol. 1968 May 1;51(5):152–161. [PMC free article] [PubMed] [Google Scholar]
  25. Taylor G. S., Paton D. M., Daniel E. E. Characteristics of electrogenic sodium pumping in rat myometrium. J Gen Physiol. 1970 Sep;56(3):360–375. doi: 10.1085/jgp.56.3.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Taylor G. S., Paton D. M., Daniel E. E. Effect of rubidium and caesium on electrogenic sodium pumping in rat myometrium. Comp Biochem Physiol A Comp Physiol. 1971 Feb 1;38(2):251–264. doi: 10.1016/0300-9629(71)90052-1. [DOI] [PubMed] [Google Scholar]
  27. Tomita T., Yamamoto T. Effects of removing the external potassium on the smooth muscle of guinea-pig taenia coli. J Physiol. 1971 Feb;212(3):851–868. doi: 10.1113/jphysiol.1971.sp009360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Whittam R., Ager M. E. Vectorial aspects of adenosine-triphosphatase activity in erythrocyte membranes. Biochem J. 1964 Nov;93(2):337–348. doi: 10.1042/bj0930337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. van Breemen C., Farinas B. R., Casteels R., Gerba P., Wuytack F., Deth R. Factors controlling cytoplasmic Ca 2+ concentration. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):57–71. doi: 10.1098/rstb.1973.0009. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Physiology are provided here courtesy of The Physiological Society

RESOURCES