The authors regretted that Fig.4 (H and I) and graphical abstract published in this manuscript were incorrect. The images in Fig.4 (H and I) from an unrelated experimental protein sample were mistakenly included as part of the rhCOLIII group. We repearted the experiment after adjusting the experimental method, but the new data did not result in consistent results. Therefore, we believe that the results of the H and I plots in Figure 4 are incorrect. However, these corrections do not affect the interpretation and conclusion of this article.
The authors would like to apologize for any inconvenience caused. The authors are committed to maintaining the highest standards of scientific integrity and appreciate the opportunity to correct these errors.
The following corrections have been made to the corrected paper:
Correction#1: We deleted the pictures and corresponding legends of Fig. 4H and I, and the other pictures in Fig.4 (A–G) remained unchanged. The new corrected Fig. 4 and legends are presented below.
In addition, the sentence “In order to further investigate the correlation between rhCOLIII and the GSTP1 protein, we used biotin-labeled rhCOLIII to observe its distribution in A2780 cells at different times (2, 4, 6, 12, and 24 h) by confocal microscopy, rhCOLIII may be colocalization with the GSTP1 protein. (Fig. 4 (H)). In addition, TEM was used to examine the subcellular structure of A2780 cells treated with rhCOLIII at different time points (0, 6, 12, and 24 h), demonstrating that rhCOLIII may enter the cells and exert biological activity (Fig. 4 (I)).” has been removed from section “3.4. rhCOLIII specifically inhibited autophagy and immune escape by regulation of GSTP1 expression”.
Fig.4. rhCOLIII specifically inhibited autophagy and immune escape by regulating GSTP1 expression. (A) KEGG pathway analysis for proteome and transcriptome combined sequencing in A2780 cells with rhCOLIII treatment. (B) KEGG pathway analysis for down-regulated genes in A2780 cells treated with rhCOLIII. (C) Spearman correlation of GSTP1 with COL3A1 expression, collagen formation, and the PI3K-AKT-mTOR pathway in the TCGA database. The immune score of the gene GSTP1 in OC in the TCGA database. P values and Spearman's correlations (rho) are indicated. (D) Expression of the GSTP1 gene in various tumor tissues with paired normal tissues, shown in the GEP1A2 database. (E) Representative figures of tissue microarray staining for GSTP1 in normal ovarian tissue and OC tissues. (F) Western blot analysis of expression of GSTP1 in A2780 cells treated with different concentrations of rhCOLIII (0, 2, 4, and 8 mg/ml). (G) Western blot for validating the knockdown and overexpression of GSTP1 in A2780 cells. Data are presented as mean ± SD of three independent experiments. The symbols ∗, ∗∗, and ∗∗∗ show P < 0.05, 0.01, and 0.001 versus the control group.
Correction#2: The sentence “Biotin Quick Labeling Kit (Frdbbio, Wuhan, Chain) was used to label rhCOLIII. Biotin was dissolved in dimethyl sulfoxide (DMSO, Solarbio, China) to a final concentration of 10 mM. Labeling buffer was used to dissolve rhCOLIII (8 mg/ml), Biotin was added and incubated at 37 °C, protected from light for 30 min, placed in an ultrafiltration tube, then centrifuged at 12,000×g for 10 min, and this step was repeated 3 times to collect biotin-labeled rhCOLIII. A2780 cells were exposed to Biotin-labeled rhCOLIII for 2, 4, 6, 12, and 24 h, tracking the distribution of labeled rhCOLIII. After fixation with PFA and blocking with 5 % normal goat serum for 30 min, cells were incubated with primary antibodies anti-GSTP1 1:100, overnight at 4 °C. Fluorescent secondary antibodies Rhodamine B isothiocyanate (RBITC) and Fluorescein 5-isothiocyanate (FITC) were added at 37 °C for 1h in darkness. DAPI solution was added to stain nucleic acids and samples were observed by confocal laser scanning microscopy.” and the subtitle “2.14. Biotin labeling and tracking of rhCOLIII” were deleted from the section “2. Materials and methods”. Correction of the serial number in the methodological section: “2.15. Animal models: Establishment and treatments” was replaced with “2.14. Animal models: Establishment and treatments”, “2.16. Immunohistochemistry and specific stains” was replaced with “2.15. Immunohistochemistry and specific stains”, “2.17. Immunofluorescent staining” was replaced with “2.16. Immunofluorescent staining”, “2.18. Transmission electron microscopy (TEM)” was replaced with “2.17. Transmission electron microscopy (TEM)”, “2.19. Second harmonic generation (SHG) microscopy” was replaced with “2.18. Second harmonic generation (SHG) microscopy”, “2.20. Quantification and testing of RNA-seq data and proteomics” was replaced with “2.19. Quantification and testing of RNA-seq data and proteomics”, “2.21. Edman degradation” was replaced with “2.20. Edman degradation”, “2.22. Liquid chromatograph mass spectrometer (LC-MS/MS)” was replaced with “2.21. Liquid chromatograph mass spectrometer (LC-MS/MS)”, “2.23. The cancer genome atlas (TCGA) database” was replaced with “2.22. The cancer genome atlas (TCGA) database”, “2.24. Statistical analysis” was replaced with “2.23. Statistical analysis”.
Correction#3: In the graphical abstract and Fig.9, we have adjusted the position of rhCOLIII to extracellular; previous studies by our team have also shown that rhCOLIII, as a biomaterial, mainly plays an important role in the extracellular matrix. The new graphical abstract and Fig.9 are presented below.
Graphical abstract (Fig.9)
Correction#4: In the fourth paragraph of the discussion, we deleted the following two sentences: “It is interesting that the immunofluorescence indicated that rhCOLIII was probably colocalized with GSTP1. Therefore, we hypothesize that there may be some relationship between rhCOLIII and GSTP1.” and “Additionally, in order to provide further evidence of colocalization of rhCOLIII with GSTP1, we performed the protein–protein docking (ZDOCK) experiment. The ZDOCK Score of rhCOLIII and GSTP1 was −1052.26. Comprehensive analysis revealed that GSTP1 and rhCOLIII could form a stable protein docking model (Supplementary Table S4).”. In addition, the supplementary table S4 (Multimedia component 5) was also deleted from section "Appendix A. Supplementary data" and "Associated Data".
The authors are committed to maintaining the highest standards of scientific integrity and appreciate the opportunity to correct these errors.