Correction for Hou et al., Therapeutic restoration of mitochondria–endoplasmic reticulum cross talk for osteoarthritis

Correction for “Therapeutic restoration of mitochondria–endoplasmic reticulum cross talk for osteoarthritis,” by Mingzhuang Hou, Yifan Ma, Yaoge Deng, Yubin Wu, Yanrun Zhu, Yang Liu, Xiaoping Li, Lili Yu, Zirui He, Yifan Wang, Shiyan Dong, Xiaowei Xia, Jianfeng Yu, Chenqi Yu, Kang Kang, Yingjie Lu, Lili Sun, Betty Y. S. Kim, Yuan Yuan, Yijian Zhang, Wen Jiang, and Xuesong Zhu, which published September 2, 2025; 10.1073/pnas.2426992122 (Proc. Natl. Acad. Sci. U.S.A. 122, e2426992122).

The authors note that Fig. 4 appeared incorrectly. “During the process of exporting from the original image to a usable one, an error occurred where images intended for Fig. 2A were mistakenly re-exported and used in Fig. 4A. This resulted in partial duplication of the mitochondrial fluorescence panels across the two figures.” The corrected figure and legend appear below.

In addition, the authors note that SI Appendix, Figs. S2, S9, and S13 appeared incorrectly. “Due to a duplication error, the β-actin in Fig. 1D (representing Intact and Damaged cartilage) was repeatedly displayed in SI Appendix, Fig. S2C (also representing Intact and Damaged cartilage). Due to incorrect image selection among different repetitions within the same group, in SI Appendix, Fig. S13D, the superimposed image of the red, green, and gray channel images does not match the image representing merge. Due to incorrect position arrangement, in SI Appendix, Fig. S9A, the positions of the green fluorescence patterns of veh-sisirt3 and sisirt3 groups were wrongly swapped.” The SI Appendix has been corrected online.

Fig. 4.

SIRT3 promotes mitochondrial fusion through MFN2 deacetylation. (A) Representative fluorescence and TEM images of mitochondria in chondrocytes treated with AAV-SIRT3 or siSIRT3. (B) Mitochondrial aspect ratio of mitochondria in chondrocytes (n = 80). (C) Measurement of mitochondrial branch length in chondrocytes (n = 400). (D and E) Representative images and quantitative analysis of mitochondrial membrane potential (n = 8). (F–H) OCR, ATP production, and maximal respiration measured by the XFe96 Seahorse Analyzer (n = 4). (I) Representative images of Safranin O/Fast Green staining and immunofluorescence for MFN2 in mice. (J) Quantitative analysis of MFN2-positive cells (n = 6). (K) Translational expression levels of extracellular matrix metabolism markers. (L) Translational expression levels of mitochondrial dynamics markers. (M) Molecular docking analysis of SIRT3 and MFN2 interaction. (N–Q) Coimmunoprecipitation assays validating the interaction between SIRT3 and MFN2. (R and S) Effects of SIRT3 on the acetylation level of MFN2. (T) The deacetylation site “K560” on MFN2 across several species. All samples were obtained at 8 wk after surgery. The mice in the sham group were selected to be fed with mice of the same age as the DMM group, and samples were taken when the mice were all 16 wk old. Data are shown as means ± SDs and statistical significance is analyzed by one-way ANOVA. Sta- tistically significant differences are indicated by P < 0.05 between the indicated groups.

Supplementary Material

Appendix 01 (PDF)