TABLE 3.
Plasmids used in this study
| Plasmid | Description | Source |
| pACT2 | Yeast vector for expressing Gal4p activation domain fusion proteins | Li et al. 1994 |
| pET15b | E. coli vector for generating His6-tagged fusion proteins | Novagen |
| pESC-URA | Yeast multicopy plasmid marked with URA3 for galactose-regulated expression | Stratagene |
| pGAD-c1 | Yeast vector for expression of Gal4p activation domain fusion proteins | James et al. 1996 |
| pGBDU-c1 | Yeast vector for expression of Gal4p DNA-binding domain fusion proteins | James et al. 1996 |
| pGEX-2T | E. coli vector for generating GST-tagged fusion proteins | Pharmacia |
| pHZ18(A) | Yeast plasmid containing galactose-inducible lacZ | Long et al. 1995 |
| pHMTc-She2p | E. coli expression vector for His6-MBP-TEV-She2p | This work |
| YCplac22 | Yeast single-copy shuttle plasmid marked with TRP1 | Gietz and Sugino 1998 |
| YCplac111 | Yeast single-copy shuttle plasmid marked with LEU2 | Gietz and Sugino 1998 |
| YEplac112 | Yeast multicopy shuttle plasmid marked with TRP1 | Gietz and Sugino 1998 |
| C3431 | YEplac195 containing ASH1 | Long et al. 1997 |
| pGEM-4Z | Vector for in vitro transcription | Promega |
| pUG6 | Plasmid for generating KAN disruption cassettes by PCR | Güldener et al. 1996 |
| pSH47 | Plasmid containing a galactose-inducible cre recombinase construct | Güldener et al. 1996 |
| pIIIA/MS2-2 | Three-hybrid vector for the expression of MS2-fusion RNAs | Zhang et al. 1997 |
| pEP13 | pACT2 containing entire She3p open reading frame | Long et al. 2000 |
| pRL80 | pIIIA/MS2-2 containing the 127-nt SmaI E3 ASH1 localization element | Long et al. 2000 |
| pRL104 | pET15b containing a C-terminal domain of She3p | This work |
| pRL168 | pGEM-3Z containing element E3 (118 nt) | Long et al. 2000 |
| pRL172 | pGEX-2T containing the open reading frame corresponding to She2p | Long et al. 2000 |
| pRL199 | YCplac111 containing She2p-myc6 | This work |
| pRL266 | pET15b containing the open reading frame corresponding to She2p | This work |
| pRL270 | Identical to pRL172 except carrying the R63K amino acid substitution in She2p | This work |
| pRL273 | Identical to pRL172 except carrying the N36S amino acid substitution in She2p | This work |
| pRL277 | pHZ18(A)/IST2 2098-3141 | This work |
| pRL322 | Identical to pRL199 except carrying an R63K amino acid substitution in She2p | This work |
| pRL324 | Identical to pRL199 except carrying an N36S amino acid substitution in She2p | This work |
| pRL418 | pGBDU-c1 containing wild-type She2p | This work |
| pRL419 | pGBDU-c1 containing N36S She2p | This work |
| pRL431 | pGBDU-c1 containing R63K She2p | This work |
| pRL441 | pGAD-c1 containing N36S She2p | This work |
| pRL442 | pGAD-c1 containing R63K She2p | This work |
| pRL445 | pGAD-c1 containing wild-type She2p | This work |
| pRL453 | YCplac111 containing wild-type She2p | This work |
| pRL460 | YCplac22 containing She3p-myc6 | This work |
| pRL462 | YCplac111 containing N36S She2p | This work |
| pRL463 | YCplac111 containing R63K She2p | This work |
| pRL552 | pGAD-c1 containing R43K She2p | This work |
| pRL553 | pGAD-c1 containing R44K She2p | This work |
| pRL554 | pGAD-c1 containing R49K She2p | This work |
| pRL555 | pGAD-c1 containing R52K She2p | This work |
| pRL669 | pGAD-c1 containing R43A She2p | This work |
| pRL670 | pGAD-c1 containing R44A She2p | This work |
| pRL671 | pGAD-c1 containing R49A She2p | This work |
| pRL672 | pGAD-c1 containing R52A She2p | This work |
| pRL673 | pGAD-c1 containing R63A She2p | This work |
| pRL676 | YCplac22 containing wild-type She2p-myc6 | This work |
| pRL677 | YCplac22 containing N36S She2p-myc6 | This work |
| pRL678 | YCplac22 containing R63K She2p-myc6 | This work |
| pRL679 | pGEM-4Z containing MS2 cloned from pIIIA/MS2-2 | This work |
| pRL718 | pESC-URA containing ASH1 | This work |
| pRL740 | YCplac111 containing R43A She2p-myc6 | This work |
| pRL741 | YCplac111 containing R44A She2p-myc6 | This work |
| pRL742 | YCplac111 containing R49A She2p-myc6 | This work |
| pRL743 | YCplac111 containing R52A She2p-myc6 | This work |
| pRL744 | YCplac111 containing R63A She2p-myc6 | This work |
| pRL745 | YCplac111 containing R43K She2p-myc6 | This work |
| pRL746 | YCplac111 containing R44K She2p-myc6 | This work |
| pRL747 | YCplac111 containing R49K She2p-myc6 | This work |
| pRL748 | YCplac111 containing R52K She2p-myc6 | This work |