Fig. 3.

Evaluation of RAA–CRISPR/Cas12a assay performance. A Detection results of five different reaction mixtures under 480 nm blue light. The “+” and “−” indicators represent the inclusion or exclusion, respectively, of specific elements in each test condition. B Kinetic fluorescence monitoring of identical reaction setups. The assay used 200 nM Cas12a protein, 200 nM crRNA, and 200 nM fluorescent-quenched ssDNA reporters. The detection template consisted of a mgc2 gene-containing plasmid from MG, where RAA amplification products act as the detection substrate for the combined RAA–CRISPR/Cas12a system