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Primers, CrRNA and probes
| Primers | Sequences (5′–3′) |
|---|---|
| F1 | GCAGGTGCTGGGTTGATTGTTGTTTCTTTAC |
| R1 | ACGTTCTTGGATCATCATTCTTTCTTTTTTC |
| F2 | GCTGGGTTGATTGTTGTTTCTTTACTCTTGG |
| R2 | ATCTTTTGGTGTTCTTCACGTTCTTGGATCA |
| F3 | GTTGATTGTTGTTTCTTTACTCTTGGGTTTA |
| R3 | AAGGGATTCAACCATCTTTTGGTGTTCTTCA |
| crRNA | uaauuucuacuaaguguagauGGGATTGGGATTCCGATCGCTAA |
| FQ-ssDNA | FAM-TTATT-BHQ1 |
| FB-ssDNA | FAM-TTTTTTTTATT-Biotin |
