Table 2.

Amplification efficiency test of RAA primers using Qsep100

Primers	Peak fragment size (bp)	Product concentration (ng/µL)	Peake area
F1/R1	92	0.47	83,645
F1/R2	108	0.15	32,654
F1/R3	120	0.48	106,322
F2/R1	86	0.55	95,955
F2/R2	102	0.50	101,944
F2/R3	114	0.69	153,155
F3/R1	80	0.51	91,163
F3/R2	97	0.54	104,662
F3/R3	109	0.67	147,369