Abstract
Sex differences in responses to chronic stress are implicated in the higher prevalence of major depression and PTSD in females. Evidence of sex differences in endocannabinoid (eCB) physiology suggests that eCB signaling contributes to sexual disparities in fear conditioning and extinction. In adolescent male Sprague-Dawley rats, exposure to chronic-mild-unpredictable stress (CMS) resulted in enhanced trace-fear conditioning that was reversed by CB1 activation (Reich et al, 2013). In the present study, we assessed the effects of CMS and CB1 activation on hippocampal-dependent trace and contextual fear conditioning in adolescent female Sprague-Dawley rats. CMS exposure enhanced trace freezing behavior during memory recall compared to non-stress controls. This effect was not observed in contextually conditioned females. The CB1 receptor agonist, ACEA (0.1 mg/kg), administered prior to trace memory recall, but not prior to acquisition, significantly decreased freezing in both stress and non-stress females. ACEA significantly reduced baseline freezing behavior during trace memory recall in both stress and non-stress rats, however ACEA either 1) did not affect or 2) impaired short and long-term extinction in stress and non-stress females. In contextually conditioned females, ACEA decreased freezing during memory recall, although the effect was more robust in stress rats. ACEA impaired long-term contextual extinction in stress females while facilitating this in non-stress controls. However, ACEA had no effect or impaired short-term contextual extinction in both stress and non-stress groups. The results demonstrate that CMS enhances hippocampal-dependent episodic fear memories but has limited effects on contextual fear conditioning in female rats. These findings have implications in the use of medical cannabinoid treatment of disorders such as PTSD, as well as recreational cannabis use in adolescent/young adult females.
Full Text Availability
The license terms selected by the author(s) for this preprint version do not permit archiving in PMC. The full text is available from the preprint server.
