Article title: Zinc promotes cell proliferation via regulating metal-regulatory transcription factor 1 expression and transcriptional activity in pulmonary arterial hypertension
Authors: Chen, A., Gao, G., Lian, G., Gong, J., Luo, L., Liu, J., … Xie, L
Journal: Cell Cycle
DOI: https://doi.org/10.1080/15384101.2023.2205209
The published version contained incorrect Figures 1, 7 and 8. The figures have now been updated, and the article has been republished. Please find below the updated Figures 1, 7 and 8. We regret for the inconvenience this might have caused.
Figure 1.
Representative zinc probe fluorescence images in PASMCs. a Elevated intensity of fluorescent indicator which reflects intracellular free zinc levels in MCT-PASMCs compared with control (n = 3). b Elevated intensity of fluorescent indicator which reflects intracellular free zinc levels in PASMCs in response to increased ZnSO4 concentration in medium (n = 3). Ctrl: control; MCT: PASMCs isolated from MCT-PAH rats. Scale bar = 25 μm.
Figure 7.
The negative effect of Mtf-1/Plgf-siRNA on MCT-PASMCs proliferation. a,b Loss of Mtf-1 downregulated the expressions of MTF-1, PlGF and PCNA in MCT-PASMCs (n = 5). c,d EdU and CCK8 assay revealed knocking down Mtf-1 reduced MCT-PASMCs proliferation (n = 5, 8). e Scratch assay showed that Mtf-1-siRNA abated MCT-PASMCs migration (n = 5). f,g Loss of Plgf downregulated the expressions of PlGF and PCNA in MCT-PASMCs (n = 5). h,i EdU and CCK8 assay revealed knocking down Plgf reduced MCT-PASMCs proliferation (n = 5, 8). j Scratch assay showed that Plgf-siRNA abated MCT-PASMCs migration (n = 5). Ctrl: control; MCT: PASMCs isolated from MCT-PAH rats; NC: negative control. *P<0.05 vs. Ctrl, #P<0.05 vs. MCT, $P<0.05 vs. MCT+NC. EdU assay scale bar = 100 μm, scratch assay scale bar = 100 μm.
Figure 8.
The negative effect of Mtf-1/Plgf-siRNA on ZnSO4-treated PASMCs proliferation. a,b Loss of Mtf-1 downregulated the expressions of MTF-1, PlGF and PCNA in ZnSO4-treated PASMCs (n = 5). c,d EdU and CCK8 assay revealed knocking down Mtf-1 reduced ZnSO4-treated PASMCs proliferation (n = 5, 8). e Scratch assay showed that Mtf-1-siRNA abated ZnSO4-treated PASMCs migration (n = 5). f,g Loss of Plgf downregulated the expressions of PlGF and PCNA in ZnSO4-treated PASMCs (n = 5). h,i EdU and CCK8 assay revealed knocking down Plgf reduced ZnSO4-treated PASMCs proliferation. (n = 5, 8). j Scratch assay showed that Plgf-siRNA abated ZnSO4-treated PASMCs migration (n = 5). Ctrl: control; ZnSO4: ZnSO4-treated PASMCs; NC: negative control. *P<0.05 vs. Ctrl, #P<0.05 vs. NC, $P<0.05 vs. ZnSO4, &P<0.05 vs. siMtf-1/Plgf. EdU assay scale bar = 100 μm, scratch assay scale bar = 100 μm.