Fig. 7.

Silencing of Akt further reduces the NF-κB signal transduction inhibited by quercetin. Lamellar keratinocytes (passages 2–4) were transfected with si-Akt or siRNA negative control (si-NC) for 36 h, then treated with LPS (10 µg/mL) and quercetin (25 µM) for 24 h. A The mRNA expression of Akt was determined with RT-qPCR. B The protein expression of Akt was detected by Western blot. C The protein expression of Akt was calculated by densitometric analysis. D The protein expression of p-PI3K, PI3K, p-Akt, and Akt was detected by Western blot. E-H The protein expression of (E) p-PI3K, (F) PI3K, (G) p-Akt, and (H) Akt was calculated by densitometric analysis. I, J The mRNA expression of (I) PI3K and (J) Akt was determined with RT-qPCR. K The protein expression of p-p65, p65, p-IκBα, and IκBα was detected by western blotting. L-O The protein expression of (L) p-p65, (M) p65, (N) p-IκBα, and (O)IκBα was calculated by densitometric analysis. P, Q The mRNA expression of (P) p65 and (Q) IκBα was determined with RT-qPCR. The data are presented as mean ± standard deviation from three independent biological replicates. The symbol “+” in the diagram indicates the addition of a substance, while “-” denotes no addition. The symbol * indicates P < 0.05, and ** indicates P < 0.01